| Description | Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography. Working Dilution: >= 1:4000 by Indirect ELISA using ALPH-conjugated secondary antibody.Western Blot: At a 1:4000 dilution, a strong reaction to 0.3 and 0.6 ug purified Form: IgG fraction from antisera obtained by ammonium sulfate precipitation and DEAE-Sephacel ion-exchange chromatography. Working Dilution: >= 1:4000 by Indirect ELISA using ALPH-conjugated secondary antibody.Western Blot: At a 1:4000 dilution, a strong reaction to 0.3 and 0.6 ug purified myeloperoxidase using ALPH-conjugated secondary antibody.IEP: Single arc versus human neutrophil granule and purified antigen.The Myeloperoxidase immunogen used to prepare this antibody was purified in the Athens Research & Technology laboratory. It is a native protein, whole molecule, isolated from human neutrophils.PLEASE NOTE: This product is a laboratory reagent and is not to be administered to humans or used for any drug purpose. For research use only.References:Gomez-Mejlba SE, et al. Radicalization of Glyceraldehyde-3-Phosphate Dehydrogenase by HOCl in Living Cells. Enz Eng 2015, 4:2... Read More | Nucleoprotein (396-404) is the 396 to 404 fragment of lymphocytic choriomeningitis virus (LCMV). Nucleoprotein (396-404) is the H-2D(b)-restricted immunodominant epitope and can be used as a molecular model of viral antigen.Form:Solid | Inquire | Purity>95% SDS-PAGEFunctionHas hepatocyte mitogenic activity | Background：Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. Rat TNF-alpha consisitsBackground：Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF2, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism. Rat TNF-alpha consisits of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD). Within the ECD, rat TNF-alpha shares 94% aa sequence identity with mouse and 69%-76% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus TNF-alpha. TNF-alpha is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF-alpha can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer of the TNF-alpha extracellular domain. TNF-alpha binds the ubiquitous 55-60 kDa TNF RI and the hematopoietic cell-restricted 80 kDa TNF RII, both of which are also expressed as homotrimers. Both type I and type II receptors bind TNF-alpha with comparable affinity, although only TNF RI contains a cytoplasmic death domain which triggers the activation of apoptosis. Soluble forms of both types of receptors are released and can neutralize the biological activity of TNF-alpha. Post-translational modificationsThe soluble form derives from the membrane form by proteolytic processing.The membrane form, but not the soluble form, is phosphorylated on serine residues.Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid... Read More |