| Description | Storage buffer: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 µg/ml HSA, pH 8.0Exonuclease III has 3 '→5' exonuclease activity on double-stranded DNA, which can degrade flat end, 3 'end dented and incised DNA, and degrade DNA molecules from the 3' end. For 3 'protruding Storage buffer: 50mM Tris, 50mM KCl, 1mM DTT, 0.05mM EDTA, 50% Glycerol, 200 µg/ml HSA, pH 8.0Exonuclease III has 3 '→5' exonuclease activity on double-stranded DNA, which can degrade flat end, 3 'end dented and incised DNA, and degrade DNA molecules from the 3' end. For 3 'protruding ends, especially protruding DNA molecules 4 nt or longer cannot be cut at all. In addition, the active site of the enzyme depends on the helical structure and varies according to the sequence (C>A=T>G).Product composition:rp216608Component5KU25KUStoragerp216608AExonuclease III50 µL250 µL-20°C. Avoid freeze/thaw cyclerp216608B10×Exonuclease III Reaction Buffer750 µL3.75 mL-20°C. Avoid freeze/thaw cycleSourceE.coliEnzyme Activity DefinitionThe amount of enzyme required to catalyze the production of 1 nmol acid-soluble total nucleotide in a 50 µl reaction system at 37℃ for 30 min is defined as 1 unit (U).Applications(1) Non-directional nested deletion;(2) Site-directed mutagenesis;(3) Preparation of chain specific probes;(4) Preparation of single-stranded substrates for dideoxy sequencing.Protocol(1) Configure the reaction system, as shown in the following tableComponentVolumeDNA5 µg10×Exonuclease III Reaction Buffer5 µlExonuclease III0.5 µlNuclease-free WaterUp to 50 µl(2) Incubation at 37 "C for 30min.(3) Incubation at 75°C for 10 min terminated the reaction.Cautions(1) The reaction temperature, the concentration of salt ions in the system and the ratio of enzyme to DNA will affect the activity of the enzyme.(2) This product is for scientific research only and shall not be used for other purposes... Read More | Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation... Read More | Purity> 96% by SDS-PAGE and HPLC analyses.FunctionHas weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, Purity> 96% by SDS-PAGE and HPLC analyses.FunctionHas weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.Post-translationalThe N-terminal processed forms HCC-1(3-74), HCC-1(4-74) and HCC-1(9-74) are produced in small amounts by proteolytic cleavage after secretion in blood. HCC-1(1-74), but not HCC-1(3-74) and HCC-1(4-74), is partially O-glycosylated; the O-linked glycan consists of one Gal-GalNAc disaccharide, further modified by two N-acetylneuraminic acids... Read More | Purity>97% SDS-PAGE and HPLC analyses. FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen Purity>97% SDS-PAGE and HPLC analyses. FunctionLA-PF4 stimulates DNA synthesis, mitosis, glycolysis, intracellular cAMP accumulation, prostaglandin E2 secretion, and synthesis of hyaluronic acid and sulfated glycosaminoglycan. It also stimulates the formation and secretion of plasminogen activator by human synovial cells. NAP-2 is a ligand for CXCR1 and CXCR2, and NAP-2, NAP-2(73), NAP-2(74), NAP-2(1-66), and most potent NAP-2(1-63) are chemoattractants and activators for neutrophils. TC-1 and TC-2 are antibacterial proteins, in vitro released from activated platelet alpha-granules. CTAP-III(1-81) is more potent than CTAP-III desensitize chemokine-induced neutrophil activation.Post-translationalProteolytic removal of residues 1-9 produces the active peptide connective tissue-activating peptide III (CTAP-III) (low-affinity platelet factor IV (LA-PF4)). Proteolytic removal of residues 1-13 produces the active peptide beta-thromboglobulin, which is released from platelets along with platelet factor 4 and platelet-derived growth factor. NAP-2(1-66) is produced by proteolytical processing, probably after secretion by leukocytes other than neutrophils. NAP-2(73) and NAP-2(74) seem not be produced by proteolytical processing of secreted precursors but are released in an active form from platelets... Read More | Purity>97% by SDS-PAGE and HPLC analyses.Additional sequence informationN-terminal Glycine.FunctionChemotactic for monocytes and T-lymphocytes. Binds to CXCR3.Post-translationalCXCL10(1-73) is produced by proteolytic cleavage after secretion from keratinocytes |