| Description | Reconstitution: After centrifugation, we suggest addition of 50 mM Na Acetate, pH 5.5, with 150 mM NaCl to the original volume, followed by gentle mixing to ensure adequate homogenization.This enzyme degrades elastin, collagen, and proteoglycan and has been implicated in the development of pulmonaryReconstitution: After centrifugation, we suggest addition of 50 mM Na Acetate, pH 5.5, with 150 mM NaCl to the original volume, followed by gentle mixing to ensure adequate homogenization.This enzyme degrades elastin, collagen, and proteoglycan and has been implicated in the development of pulmonary emphesema and rheumatoid arthritis.Activity: 20-22 units per mg protein. One unit is defined as the amount of enzyme that hydrolyzes one umole of MeO-Suc-Ala-Ala-Pro-Val-pNA per minute at 25℃, pH 7.5. Product Citations:Mittendorf, Elizabeth A., Gheath Alatrash, Na Qiao, Yun Wu, Pariya Sukhumalchandra, Lisa S. St John, Anne V. Philips et al. "Breast cancer cell uptake of the inflammatory mediator neutrophil elastase triggers an anticancer adaptive immune response." Cancer research 72, no. 13 (2012): 3153-3162van den Berg, Carmen W., et al. "Mechanism of Neutrophil Dysfunction: Neutrophil Serine Proteases Cleave and Inactivate the C5a Receptor." The Journal of Immunology 192.4 (2014): 1787-1795.Edwards, J. Vincent, Nicolette Prevost, Kandan Sethumadhavan, Abul Ullah, and Brian Condon. "Peptide conjugated cellulose nanocrystals with sensitive human neutrophil elastase sensor activity." Cellulose (2013): 1-13.Valdez C, Wong YC, Schwake M, Bu G, Wszolek ZK, Krainc D.Progranulin-mediated deficiency of cathepsin D results in FTD and NCL-like phenotypes in neurons derived from FTD patients.Hum Mol Gen. 2017. 2017 Sep 25. doi:10.1093/hmg/ddx364Kerros C, et al.Neuropilin-1 mediates neutrophil elastase uptake and cross-presentation in breast cancer cells.J Biol Chem. 2017 Jun 16;292(24):10295-10305. doi: 10.1074/jbc.M116.773051. Epub 2017 May 3.Martin KR, Kantari-Mimoun C, Yin M, Pederzoli-Ribeil M, et al.Proteinase 3 Is a Phosphatidylserine-binding Protein That Affects the Production and Function of Microvesicles.J Biol Chem. 2016 May 13;291(20):10476-89. doi: 10.1074/jbc.M115.698639. Epub 2016 Mar 9.Edwards JV, Fontenot KR, Prevost NT, Pircher N, Liebner F, Condon BD.Preparation, Characterization and Activity of a Peptide-Cellulosic Aerogel Protease Sensor from Cotton.Sensors (Basel). 2016 Oct 26;16(11). pii: E1789.Chawla A, Alatrash G, Philips AV, Qiao N, et al.Neutrophil elastase enhances antigen presentation by upregulating human leukocyte antigen class I expression on tumor cells.Cancer Immunol Immunother. 2016 Jun;65(6):741-51. doi: 10.1007/s00262-016-1841-6. Epub 2016 Apr 29.Sinden NJ, et al.α-1-Antitrypsin variants and the proteinase/antiproteinase imbalance in chronic obstructive pulmonary disease.Am J Physiol Lung Cell Mol Physiol. 2015 Jan 15;308(2):L179-90.Ksiazek M, et al.Miropin, a Novel Bacterial Serpin from the Periodontopathogen Tannerella forsythia, Inhibits a Broad Range of Proteases by Using Different Peptide Bonds within the Reactive Center Loop.J Biol Chem. 2015 Jan 2;290(1):658-70. doi: 10.1074/jbc.M114.601716. Epub 2014 Nov 11.Ramadass M, Ghebrehiwet B, Kew RR.Enhanced recognition of plasma proteins in a non-native state by complement C3b. A possible clearance mechanism for damaged proteins in blood.Mol Immunol. 2015 Mar;64(1):55-62. doi: 10.1016/j.molimm.2014.10.022. Epub 2014 Nov 15.Thomas MP, et al.Leukocyte Protease Binding to Nucleic Acids Promotes Nuclear Localization and Cleavage of Nucleic Acid Binding Proteins.J Immunol. 2014 Jun 1;192(11):5390-7. doi: 10.4049/jimmunol.1303296. Epub 2014 Apr 25.Ungurs MJ, Sinden NJ, Stockley RA.Progranulin is a substrate for neutrophil-elastase and proteinase-3 in the airway and its concentration correlates with mediators of airway inflammation in COPDAm J Physiol Lung Cell Mol Physiol. 2014 Jan 1;306(1):L80-7. doi: 10.1152/ajplung.00221.2013. Epub 2013 Nov 1.Sinden NJ, Koura F, Stockley RA.The significance of the F variant of alpha-1-antitrypsin and unique case report of a PiFF homozygote.BMC Pulm Med. 2014 Aug 7;14:132. doi: 10.1186/1471-2466-14-132.Kenninston JA, et al.Inhibition of Plasma Kallikrein by a Highly Specific Active Site Blocking Antibody.J Biol Chem. 2014 Aug 22;289(34):23596-608. doi: 10.1074/jbc.M114.569061. Epub 2014 Jun 26.Tejera P, et al.Functional Characterization of Polymorphisms in the Peptidase Inhibitor 3 (Elafin) Gene and Validation of Their Contribution to Risk of Acute Respiratory Distress Syndrome.Am J Respir Cell Mol Biol. 2014 Aug;51(2):262-72. doi: 10.1165/rcmb.2013-0238OC.Zheng K, et al.Influence of glycosylation pattern on the molecular properties of monoclonal antibodies.MAbs. 2014 May-Jun;6(3):649-58. doi: 10.4161/mabs.28588. Epub 2014 Mar 24... Read More | IRAK-4 protein kinase inhibitor 2 (compound 1) is a potent inhibitor of interleukin-1 (IL-1) receptor-associated kinase-4 (IRAK-4), with an IC 50 of 4 µM. IRAK-4 protein kinase inhibitor 2 can be used for the research of inflammatory and immune-related conditions or disordersIn VitroIRAK-4 IRAK-4 protein kinase inhibitor 2 (compound 1) is a potent inhibitor of interleukin-1 (IL-1) receptor-associated kinase-4 (IRAK-4), with an IC 50 of 4 µM. IRAK-4 protein kinase inhibitor 2 can be used for the research of inflammatory and immune-related conditions or disordersIn VitroIRAK-4 protein kinase inhibitor 2 (compound 1) also inhibits IRAK-1, with an IC 50 of <10 µM. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:SolidIC50& Target:IRAK4 4 µM (IC 50 )... Read More | Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ Purity>95% SDS-PAGE.FunctionB Cell Activating Factor Receptor (BAFF-R), also named tumor necrosis factor receptor superfamily member 13C, is a member of the TNFR superfamily. It is highly expressed in spleen, lymph node, and resting B cells and to some extent in activated B cells, resting CD4+ cells and peripheral blood leukocytes. BAFF receptor is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain and binds with high specificity to BAFF (TNFSF13B). It enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. BAFF receptor/BAFF signaling plays a critical role in B cell survival and maturation... Read More | Purity:>95%(SDS-PAGE) Function:Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface Purity:>95%(SDS-PAGE) Function:Cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Up-regulates cell surface molecules, including adhesion molecules.Background:CD14 is a 55 kDa cell surface glycoprotein that is preferentially expressed on monocytes/macrophages. The human CD14 cDNA encodes a 375 amino acid (aa) residue precursor protein with a 19 aa signal peptide and a C-terminal hydrophobic region characteristic for glycosylphosphatidyinositol (GPI)-anchored proteins. Human CD14 has four potential N-linked glycosylation sites and also bears O-linked carbohydrates. The amino acid sequence of human CD14 is approximately 65% identical with the mouse, rat, rabbit, and bovine proteins. CD14 is a pattern recognition receptor that binds lipopolysaccharides (LPS) and a variety of ligands derived from different microbial sources. The binding of CD14 with LPS is catalyzed by LPS-binding protein (LBP). The toll-like-receptors have also been implicated in the transduction of CD14-LPS signals. Similar to other GPI-anchored proteins, soluble CD14 can be released from the cell surface by phosphatidyinositol-specific phospholipase C. Soluble CD14 has been detected in serum and body fluids. High concentrations of soluble CD14 have been shown to inhibit LPS-mediated responses. However, soluble CD14 can also potentiate LPS response in cells that do not express cell surface CD14... Read More | Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase... Read More |