| Description | Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description:1. This product is composed of 11 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 15 kb, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp,Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description:1. This product is composed of 11 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 15 kb, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, 5000 bp, 8000 bp, and 15000 bp. The 500 bp and 1500 bp bands are highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.1. This product consists of 9 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 5000 bp, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, and 5000 bp. The 500 bp and 1500 bp bands are highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.2. In 5 ul of this product, the content of the regular bands is approximately 30 ng, while the highlighted band contains about 75 ng.3. The product is preserved in 1x Loading Buffer and can be directly used for electrophoresis, making it convenient to use.4. This product is not suitable for polyacrylamide gel electrophoresis.Recommended Electrophoresis Buffer and Agarose Gel Concentration:This product is recommended to be used with 1x TAE electrophoresis buffer, with a suggested agarose concentration of 1.0% to 1.5%. For electrophoresis of smaller fragments, it is recommended to use GelRed nucleic acid dye.Usage Instructions:1. Prepare an agarose gel containing a nucleic acid dye, such as EB or GelRed.2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.0% to 1.5%.3. It is suggested to use 1x TAE buffer, with an electrophoresis voltage not exceeding 10 v/cm.4. For common 3.5 mm sample wells, a loading volume of 3 to 5 ul is recommended, with an appropriate increase for wider gel wells.5. Run the electrophoresis to the appropriate distance: For nucleic acid dyes such as EB, Goldview, and GRBlue, the bromophenol blue front should not exceed two-thirds of the gel, otherwise, smaller fragments may weaken due to the detachment of the nucleic acid dye from DNA. For dyes like GelRed, longer distances can be used as long as the smallest fragment does not run out of the gel. Generally, the bromophenol blue indicator band should be at least 1 cm away from the edge of the gel.6. After electrophoresis, observe the bands under a UV lamp.7. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, containing both bromophenol blue and xylene cyanol as indicators.Product componentD745353Component100 T500TStorageD745353ADNA Ladder (100-15000bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.D745353B5xLoading buffer1mL5×1 mL-20℃. Avoid freeze/thaw cycle... Read More | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：IL12 is a cytokine that acts on T and natural killer cells, and has a broad array of biological activities. It is a disulfide-linked heterodimer composed of the 40 kD cytokine receptor like subunit and a 35 kD subunit. This cytokine is expressed by activated macrophages that serve as an essential inducer of Th1 cells development. IL12 has been found to be important for sustaining a sufficient number of memory/effector Th1 cells to mediate long-term protection to an intracellular pathogen. Recombinant human IL12 protein, fused to His-tag at C-terminus, was expressed in insect cells using baculovirus expression system and purified by using conventional chromatography techniques... Read More | Recombinant Human Serum Albumin (rHSA) is an active compound and possesses an identical conformation to plasma derived HSA. Recombinant Human Serum Albumin (rHSA) has no difference between rHSA and plasma derived HSA. Recombinant Human Serum Albumin (rHSAAppearance:SolidBiological Activity:Recombinant Human Serum Albumin (rHSA) is an active compound and possesses an identical conformation to plasma derived HSA. Recombinant Human Serum Albumin (rHSA) has no difference between rHSA and plasma derived HSA. Recombinant Human Serum Albumin (rHSAAppearance:SolidBiological Activity:Recombinant Human Serum Albumin (rHSA) is an active compound and possesses an identical conformation to plasma derived HSA. Recombinant Human Serum Albumin (rHSA) has no difference between rHSA and plasma derived HSA. Recombinant Human Serum Albumin (rHSA... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TREPurity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptor-regulated SMAD (R-SMAD) that is an intracellular signal transducer and transcriptional modulator activated by TGF-beta (transforming growth factor) and activin type 1 receptor kinases. Binds the TRE element in the promoter region of many genes that are regulated by TGF-beta and, on formation of the SMAD3/SMAD4 complex, activates transcription. Also can form a SMAD3/SMAD4/JUN/FOS complex at the AP-1/SMAD site to regulate TGF-beta-mediated transcription. Has an inhibitory effect on wound healing probably by modulating both growth and migration of primary keratinocytes and by altering the TGF-mediated chemotaxis of monocytes. This effect on wound healing appears to be hormone-sensitive. Regulator of chondrogenesis and osteogenesis and inhibits early healing of bone fractures. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description:Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. A35R gene is highly conserved among poxviruses and encodes a previously uncharacterized hydrophobic acidicPurity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description:Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. A35R gene is highly conserved among poxviruses and encodes a previously uncharacterized hydrophobic acidic protein. The A35R has little homology to any protein outside of poxviruses, suggesting a novel virulence Monkeypox is a zoonotic disease caused by monkeypox virus (MPXV), which is a member of orthopoxvirus genus. A35R gene is highly conserved among poxviruses and encodes a previously uncharacterized hydrophobic acidic protein. The A35R has little homology to any protein outside of poxviruses, suggesting a novel virulence mechanism.A35R could block some stage of antigen processing or presentation in infected cells or interfere with regulation of apoptosis. In addition, the A35R function may be required for growth in certain cell types, e.g., macrophage, in vivo. It localizes to factories where viral DNA is located and it was shown to be a constitutive transcriptional activator in a large-scale yeast two-hybrid study... Read More |