| Description | Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description:1. This product is composed of 11 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 15 kb, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp,Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description:1. This product is composed of 11 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 15 kb, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, 5000 bp, 8000 bp, and 15000 bp. The 500 bp and 1500 bp bands are highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.1. This product consists of 9 linear double-stranded DNA fragments, with sizes ranging from 100 bp to 5000 bp, specifically 100 bp, 250 bp, 500 bp, 750 bp, 1000 bp, 1500 bp, 2000 bp, 3000 bp, and 5000 bp. The 500 bp and 1500 bp bands are highlighted with a concentration 2.5 times higher than the other bands, facilitating observation after electrophoresis.2. In 5 ul of this product, the content of the regular bands is approximately 30 ng, while the highlighted band contains about 75 ng.3. The product is preserved in 1x Loading Buffer and can be directly used for electrophoresis, making it convenient to use.4. This product is not suitable for polyacrylamide gel electrophoresis.Recommended Electrophoresis Buffer and Agarose Gel Concentration:This product is recommended to be used with 1x TAE electrophoresis buffer, with a suggested agarose concentration of 1.0% to 1.5%. For electrophoresis of smaller fragments, it is recommended to use GelRed nucleic acid dye.Usage Instructions:1. Prepare an agarose gel containing a nucleic acid dye, such as EB or GelRed.2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.0% to 1.5%.3. It is suggested to use 1x TAE buffer, with an electrophoresis voltage not exceeding 10 v/cm.4. For common 3.5 mm sample wells, a loading volume of 3 to 5 ul is recommended, with an appropriate increase for wider gel wells.5. Run the electrophoresis to the appropriate distance: For nucleic acid dyes such as EB, Goldview, and GRBlue, the bromophenol blue front should not exceed two-thirds of the gel, otherwise, smaller fragments may weaken due to the detachment of the nucleic acid dye from DNA. For dyes like GelRed, longer distances can be used as long as the smallest fragment does not run out of the gel. Generally, the bromophenol blue indicator band should be at least 1 cm away from the edge of the gel.6. After electrophoresis, observe the bands under a UV lamp.7. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, containing both bromophenol blue and xylene cyanol as indicators.Product componentD745353Component100 T500TStorageD745353ADNA Ladder (100-15000bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.D745353B5xLoading buffer1mL5×1 mL-20℃. Avoid freeze/thaw cycle... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:Bcl-2 family proteins contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four Bcl-2 homology domains. Certain members (Bcl-2, Bcl-XL and Mcl-1) arePurity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:Bcl-2 family proteins contribute to programmed cell death or apoptosis. It is a large protein family and all members contain at least one of four Bcl-2 homology domains. Certain members (Bcl-2, Bcl-XL and Mcl-1) are antiapoptotic, whilst others (Bax, Bak, Bok) are proapoptotic... Read More | Product IntroductionHuman epidermal growth factor (EGF) has a molecular weight of 6 KD, contains 53 amino acids, and has three intramolecular disulfide bonds.Upon binding to EGFR, a specific receptor located on the cell surface, EGF causes a series of biochemical reactions in the cell: increasing Product IntroductionHuman epidermal growth factor (EGF) has a molecular weight of 6 KD, contains 53 amino acids, and has three intramolecular disulfide bonds.Upon binding to EGFR, a specific receptor located on the cell surface, EGF causes a series of biochemical reactions in the cell: increasing the level of calcium ion concentration in the cell, promoting the progress of glycolysis, increasing the synthesis of proteins, and can enhance the expression of EGFR, a specific class of genes to promote DNA synthesis and cell proliferation.Specification parametersSource Pichia pastorisAppearance white lyophilized powderActivity ≥1.0×106IU/mgpH 6.5-7.5Molecular weight 6.5kDEndotoxin ≦1.0 EU/mgCAS No 62253-63-8Matters needing attentionReconstitution: reconstitution of REGF lyophilized powder to 100-200 µg/ml with sterile water is recommended and further dilution with other solvents may be performed.REGF dissolved at 4 ° C can be stored for 2-7 days and used up as soon as possible.To not use for short periods, store at - 20 ℃.Use as soon as possible after opening to avoid contamination.Limitations of useIt is suitable for research, laboratory and production use only and cannot be used directly in humans... Read More | Product IntroductionKGF keratinocyte growth factor (KGF), a cytokine identified by Rubin et al (1989) from the culture supernatant of embryonic lung fibroblasts, is an FGF family member, namely FGF-7.KGF is secreted by stromal cells and its receptor is distributed in epithelial cells, where it is a Product IntroductionKGF keratinocyte growth factor (KGF), a cytokine identified by Rubin et al (1989) from the culture supernatant of embryonic lung fibroblasts, is an FGF family member, namely FGF-7.KGF is secreted by stromal cells and its receptor is distributed in epithelial cells, where it is a potent epithelial cell specific growth factor, and its mitogenic activity is mainly expressed in keratinocytes, which can specifically promote epithelial cell proliferation, migration and differentiation, and is closely related to many aspects, such as organ development, wound repair, tumorigenesis and immune reconstitution.Osrhkgf was created using genetic recombination, expressed from rice endosperm cells and through a protein purification process.Specification parametersSource Oryza sativaAppearance white lyophilized powderActivity ≥1.0×105IU/mgpH 6.5-7.5Molecular weight 19.0 kDEndotoxin ≦0.1EU/ugCAS No 148348-15-6Matters needing attentionReconstitution: it is recommended to lyophilize the powder of osrhkgf to 100-200 UG/ml with sterile water to make further dilutions with other solvents.The dissolved osrhkgf could be stored for 2-7 days at 4 ◦ C and used up as soon as possible.To not use for short periods, store at - 20 ℃.Use as soon as possible after opening to avoid contamination.Limitations of useIt is suitable for research, laboratory and production use only and cannot be used directly in humans... Read More | Purity>98% by SDS-PAGE and HPLC analyses.Additional sequence informationBelongs to the intercrine alpha (chemokine CxC) family.FunctionActs as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in Purity>98% by SDS-PAGE and HPLC analyses.Additional sequence informationBelongs to the intercrine alpha (chemokine CxC) family.FunctionActs as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.Post-translationalGlycosylated... Read More |