| Description | Product Description:1. This product consists of six linear double-stranded DNA fragments with a size range of 100bp to 2000bp, specifically 100bp, 250bp, 500bp, 750bp, 1000bp, and 2000bp. The 750bp band serves as an intensified indicator band, with a concentration 2.5 times higher than that of the Product Description:1. This product consists of six linear double-stranded DNA fragments with a size range of 100bp to 2000bp, specifically 100bp, 250bp, 500bp, 750bp, 1000bp, and 2000bp. The 750bp band serves as an intensified indicator band, with a concentration 2.5 times higher than that of the other bands, facilitating observation after electrophoresis.2. In 5µl of this product, the content of the regular bands is approximately 30ng, while the content of the intensified band is about 75ng. 3. This product is already preserved in a 1x Loading Buffer and can be directly used for electrophoresis, offering convenience in use.4. Both this product and the accompanying 5x Loading Buffer contain the Gelred nucleic acid stain. When used together, after electrophoresis, the bands can be directly observed under ultraviolet light without the need for subsequent staining procedures.5. This product is not suitable for polyacrylamide gel electrophoresis.6. Recommended Electrophoresis Buffer and Agarose Gel Concentration: 1x TAE electrophoresis buffer Agarose concentration: 1.5% to 2.0%.7. Using this product system, there is no need to add any nucleic acid dye in agarose gel.Usage Instructions:1. Prepare an agarose gel of the appropriate concentration without any nucleic acid stains.2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.5% to 2.0%.3. It is suggested to use 1x TAE buffer for electrophoresis, with a voltage not exceeding 10v/cm.4. For common 3.5mm sample wells, the recommended volume of DNA marker is 3 to 5µl. For wider gel wells, the sample volume should be appropriately increased.5. Mix the samples to be tested with the accompanying 5x Loading Buffer at a ratio of approximately 4:1, and then load into the gel sample wells.6. Run the electrophoresis to an appropriate distance:Since Gelred binds firmly to DNA, it is possible to fully utilize the length of the gel and run a longer distance, as long as the smallest fragment does not run out of the gel, which is beneficial for the separation of small fragments. Generally, the bromophenol blue indicator band should be no less than 1cm away from the edge of the gel.7. After electrophoresis, observe the electrophoresis bands under a UV lamp.8. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, and it contains both bromophenol blue and xylene cyan FF as dual indicators.9. If there are a large number of samples that can be directly loaded for electrophoresis testing, it is recommended to use the Gelred gel method for detection, without pre-mixing the samples, which can greatly save experimental time.Product componentG751634Component100 T500TStorageG751634AGelred-prestained DNA Ladder (100-2000bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.G751634BGelred-prestained 5xLoading buffer 500 µL5×500 µL-20℃. Avoid freeze/thaw cycle... Read More | Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a heat shock protein 70 (HSP70) inhibitor to study its effects on glutamine-induced HSP70 and inflammatory mediator release... Read More | Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from theC-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0.Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is Carboxypeptidase B catalyzes hydrolysis of the basic amino acids lysine, arginine and histidine from theC-terminal end of polypeptides. The molecular weight is 34,500 daltons, the pH optimum is 8.0, and pI is 6.0.Carboxypeptidase B is competitively inhibited by arginine and lysine. The enzyme is also inhibited by metal chelating agents, e.g., EDTA. Recombinant Carboxypeptidase B (EC 3.4.17.2) is expressed in E.Coli and purified by high pressure liquid chromatography. There is no trace of other enzyme (such as carboxypeptidase A and chymotrypsin) activity. No protease inhibitors such as PMSF are present in the preparation.Animal origin free:eliminate the risk of virus presence, or of any other potential adventitious agents found in animal-derived carboxypeptitase B.Stability:A sterile recombinant carboxypeptidase B lyophilized eliminates the risk of contamination and decreases the chances of activity loss in the process of transport and storage. High purity:1) Recombinant carboxypeptidase B provides increased specific activity and eliminates contaminating protease activities found in extracted enzymes with lower purity level. 2) No other contaminating proteases such as chymotrypsin and carboxypeptidase A. 3）Less than 10ppm of recombinant trypsin... Read More | Purity>95% SDS-PAGE.FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose Purity>95% SDS-PAGE.FunctionImportant adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.Post-translationalHydroxylated Lys-33 was not identified in PubMed:16497731, probably due to poor representation of the N-terminal peptide in mass fingerprinting. HMW complexes are more extensively glycosylated than smaller oligomers. Hydroxylation and glycosylation of the lysine residues within the collagene-like domain of adiponectin seem to be critically involved in regulating the formation and/or secretion of HMW complexes and consequently contribute to the insulin-sensitizing activity of adiponectin in hepatocytes. O-glycosylated. Not N-glycosylated. O-linked glycans on hydroxylysines consist of Glc-Gal disaccharides bound to the oxygen atom of post-translationally added hydroxyl groups. Sialylated to varying degrees depending on tissue. Thr-22 appears to be the major site of sialylation. Higher sialylation found in SGBS adipocytes than in HEK fibroblasts. Sialylation is not required neither for heterodimerization nor for secretion. Not sialylated on the glycosylated hydroxylysines. Desialylated forms are rapidly cleared from the circulation... Read More | Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary cofactor. By using the apoenzyme prepared from cells grown on a vitamin B6 deficient medium pyridoxal phosphate may be determined. The Tyrosine decarboxylase catalyzes the removal of the carboxyl group from tyrosine to produce tyramine and carbon dioxide. Pyridoxal 5'-phosphate is a necessary cofactor. By using the apoenzyme prepared from cells grown on a vitamin B6 deficient medium pyridoxal phosphate may be determined. The HOLOenzyme may be used to determine tyrosine, phenylalanine and dihydroxyphenylalanine either manometrically or colorimetrically.L-Tyrosine decarboxylase apoenzyme from Streptococcus faecalis has been used in a study to purify and characterize tyrosine decarboxylase and aromatic-L-amino-acid decarboxylase.L-Tyrosine decarboxylase apoenzyme from Streptococcus faecalis has also been used in a study to investigate the stereospecificity of sodium borohydride reduction of tyrosine decarboxylase... Read More |