| Description | Storage buffer: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description:1. This product is composed of eleven linear double-stranded DNA fragments with a size range of 100bp to 1500bp, specifically 100bp, 200bp, 300bp, Storage buffer: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description:1. This product is composed of eleven linear double-stranded DNA fragments with a size range of 100bp to 1500bp, specifically 100bp, 200bp, 300bp, 400bp, 500bp, 600bp, 700bp, 800bp, 900bp, 1000bp, and 1500bp. The 500bp band serves as an intensified indicator band, with a concentration 2.5 times higher than that of the other bands, facilitating observation after electrophoresis.2. In 5µl of this product, the content of the regular bands is approximately 30ng, while the content of the intensified band is about 75ng. 3. This product is already preserved in a 1x Loading Buffer and can be directly used for electrophoresis, offering convenience in use.4. Both this product and the accompanying 5x Loading Buffer contain the Gelred nucleic acid stain. When used together, after electrophoresis, the bands can be directly observed under ultraviolet light without the need for subsequent staining procedures.5. This product is not suitable for polyacrylamide gel electrophoresis.6. Recommended Electrophoresis Buffer and Agarose Gel Concentration: 1x TAE electrophoresis buffer Agarose concentration: 1.5% to 2.0%.7. Using this product system, there is no need to add any nucleic acid dye in agarose gel.Usage Instructions:1. Prepare an agarose gel of the appropriate concentration without any nucleic acid stains.2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.5% to 2.0%.3. It is suggested to use 1x TAE buffer for electrophoresis, with a voltage not exceeding 10v/cm.4. For common 3.5mm sample wells, the recommended volume of DNA marker is 3 to 5µl. For wider gel wells, the sample volume should be appropriately increased.5. Mix the samples to be tested with the accompanying 5x Loading Buffer at a ratio of approximately 4:1, and then load into the gel sample wells.6. Run the electrophoresis to an appropriate distance:Since Gelred binds firmly to DNA, it is possible to fully utilize the length of the gel and run a longer distance, as long as the smallest fragment does not run out of the gel, which is beneficial for the separation of small fragments. Generally, the bromophenol blue indicator band should be no less than 1cm away from the edge of the gel.7. After electrophoresis, observe the electrophoresis bands under a UV lamp.8. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, and it contains both bromophenol blue and xylene cyan FF as dual indicators.9. If there are a large number of samples that can be directly loaded for electrophoresis testing, it is recommended to use the Gelred gel method for detection, without pre-mixing the samples, which can greatly save experimental time.Product componentR751624Component100 T500TStorageR751624AGelred-prestained DNA Ladder (100-1500bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.R751624BGelred-prestained 5xLoading buffer 500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle... Read More | Activated Protein C (390-404), human TFA, a peptide of the activated protein C (a vitamin K-dependent serine protease), potently inhibits APC anticoagulant activity | Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a heat shock protein 70 (HSP70) inhibitor to study its effects on glutamine-induced HSP70 and inflammatory mediator release... Read More | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：ER alpha (Estrogen receptor alpha; also Estradiol receptor and NR3A1) is a 65-70 kDa member of the NR3 subfamily, nuclear hormone receptor family of proteins. It is widely expressed, and serves as a strong activator of estrogen-responsive genes. ER alpha is normally quiescent and bound to heat-shock proteins and immunophilins. Following beta -estradiol binding, it becomes activated, either homodimerizes or heterodimerizes with ER beta, and binds to DNA with multiple coactivators. Human ER alpha is 595 amino acids (aa) in length. It contains a DNA binding region (aa 185-250), three NLSs (aa 256-260; 266-271; 299-303), a steroid-binding site (aa 351-543), a dimerization motif (aa 497-518), and an O-GlcNAc attachment around Thr575. Major phosphorylation sites exist at Tyr537, Ser167 and Ser118. Multiple splice forms exist. There is an 80 kDa isoform that shows a substitution (duplication) of aa 412-517 for Asp411, a second isoform with a deletion of aa 255-366, a third isoform with a deletion of aa 152-412, and a fourth isoform that shows a Thr substitution for aa 152-595. Human ER alpha is only 46% aa identical to human ER beta. Over aa 1-116, human ER alpha shares 85% aa identity with mouse ER alpha... Read More | Purity>90% SDS-PAGE.Background:Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FSH), ThyroidPurity>90% SDS-PAGE.Background:Luteinizing Hormone (LH) is a 42 kDa heterodimer belonging to the glycoprotein hormone family. It is composed of noncovalently linked glycosylated alpha and beta chains. The alpha subunit (CG alpha ) is also a component of Follicle-Stimulating Hormone (FSH), Thyroid-Stimulating Hormone, and Chorionic Gonadotropin. The unique beta subunit confers the protein’s specific biological action and is responsible for the interaction with its receptor. The approximately 20 kDa human CG alpha subunit shares 73% and 72% amino acid (aa) sequence identity with the mouse and rat orthologs, respectively. The approximately 18 kDa human LH beta subunit shares 71% and 72% aa sequence identity with the mouse and rat orthologs, respectively. Multiple isoforms of LH exist due to differences in the post-translational glycosylation, sialylation, and sulphation modifications of its subunits. The composition, longevity, and activity of the different LH isoforms vary throughout a woman’s menstrual cycle and reproductive life cycle. LH is produced and secreted by the anterior pituitary gland. Its secretion is controlled by Gonadotropin-Releasing Hormone from the hypothalamus; however, LH secretion can also be stimulated by estradiol. LH works in concert with FSH to regulate female reproduction; FSH stimulates follicular growth and LH induces ovulation. LH also drives formation of the corpus luteum by promoting progesterone production. Additionally, LH has been suggested to stimulate the adrenal gland in postmenopausal women to induce secretion of sulfated DHEA, a precursor to androgens. In the testis, LH induces Leydig cell production of testosterone. Hypersecretion of LH has been shown to occur in women with polycystic ovary syndrome and is associated with an increased risk of infertility and miscarriage. Additionally, increased serum LH levels are associated with decreased cognition and have been implicated in the development and progression of Alzheimer’s disease. receptor into an A-frame... Read More |