| Description | Physical appearance: LiquidStorage buffer solution: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description1. This product is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 5000bp, Physical appearance: LiquidStorage buffer solution: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description1. This product is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 5000bp, including 100bp, 250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 3000bp, and 5000bp. 500bp and 1500bp are highlighted bands with concentrations 2.5 times higher than other bands, making them easier to observe after electrophoresis.2. In this product, the content of regular stripes is about 30ng, and the content of bright stripes is about 75ng.3. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis, making it easy to use.4. This product and its accompanying 5xLoading buffer already contain Gelred nucleic acid dye, which can be used in conjunction. After electrophoresis, it can be directly observed under UV without the need for subsequent staining treatment.5. This product is not suitable for polyacrylamide gel electrophoresis.6. Recommended concentration of electrophoresis buffer and agarose gel:1xTAE electrophoresis bufferAgarose concentration: 1.5%~2.0%7. Using this product system, no nucleic acid dye is needed in agarose gel.instructions1. Prepare agarose gel with appropriate concentration and without any nucleic acid dye.2. The concentration of gel has a great influence on DNA electrophoresis. The recommended concentration of agarose gel for this product is 1.5%~2.0%.3. It is recommended to use 1xTAE buffer with an electrophoresis voltage not exceeding 10v/cm.4. The commonly used 3.5mm sample well requires a recommended amount of 3-5 µ l for DNA markers, and the sample size should be appropriately increased for wide gel wells.5. Mix the sample to be tested with the matching 5xLoading buffer in a ratio of about 4:1, and then add it to the gel sampling hole.6. Electrophoretic to appropriate distance:Because Gelred is firmly bound to DNA, it can make full use of the length of gel, and the electrophoresis distance is longer, as long as the smallest segment does not run out of the gel, so as to facilitate the electrophoresis separation of small segments. Generally, the distance between the bromophenol blue indicator band and the edge of gel shall not be less than 1cm.7. After electrophoresis, observe the electrophoresis bands under ultraviolet light.8. The 5x loading buffer included in the product is used for sampling after mixing with the sample to be tested, and contains dual indicators of bromophenol blue and xylene blue.9. If there are a large number of samples that can be directly sampled and require electrophoresis detection, it is recommended to use Gelred gel method for detection without pre mixing samples, which can save a lot of experimental time. Product componentR751622Component100 T500TStorageR751622AGelred-prestained DNA Ladder (100-5000bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.R751622BGelred-prestained 5xLoading buffer 500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle... Read More | A general purpose purified albumin, suitable for Westerns, enzyme systems and as a protein supplement | Ganglioside GT1b is a brain ganglioside. It is composed of a neutral tetra-saccharide core, with one or two sialic acid on the internal galactose and an extra sialic acid on the non-reducing terminal of galactose | ProductsThis product is a high purity genomic DNA extract from 293T cells, agarose gel (0.7%) electrophoresis showed that the size of the DNA extract is more than 15Kb, and basically no degradation, the product is ultimately preserved in TE Buffer, which can be widely used in molecular biology ProductsThis product is a high purity genomic DNA extract from 293T cells, agarose gel (0.7%) electrophoresis showed that the size of the DNA extract is more than 15Kb, and basically no degradation, the product is ultimately preserved in TE Buffer, which can be widely used in molecular biology experiments, such as PCR, enzyme digestion, hybridization, microarray analysis, and other molecular biology experiments.The product was quantified using NanoDrop One at a concentration of 200 ng/µL.Preparation and precautions before useLong-term storage at -20˚C is recommended. Before use, the bottle should be removed from the refrigerator and equilibrated to room temperature and centrifuged before opening the cap for use. Samples should be restored to the sealed state as soon as possible after opening.How to use (take qPCR experiment as an example)1. Amplification template preparationThe samples to be detected were diluted with TE (10 mM Tris-Cl, pH 8.0,1 mM EDTA), and the concentration after dilution was as close as possible to the range of 0.05-10 ng/µL. The samples were placed on ice at 4°C and set aside.2. Standard dilution: according to the following table, firstly dilute Human DNA Standard 1 (100ng/uL) with TE to make 5 different concentrations of standards according to the table below. 10ng/µL of DNA Standard 1 (Std. 1) can be stored stably at -20℃ for 1 month; Std2-5 can only be used on the same day, and should be placed at 4℃ or on ice when not in use for the time being after preparation. When not used temporarily after preparation, it should be stored at 4℃ or on ice.styleCorresponding concentration (ng/µL)Minimum dilution volume (in µL)Std.11010 [100 ng/µL DNA Standard 1] + 90 TEStd.22.520 [Std. 1] +60 TEStd.30.62520 [Std. 2] +60 TEStd.40.1562520 [Std. 3] +60 TEStd.50.039062520 [Std. 4] +60 TE3. qPCR reaction system preparationThe cryopreserved reagents to be used were completely thawed and mixed by inversion several times before preparation, and then briefly centrifuged and prepared for use. 20 µL of the base reaction system was as follows.The base reaction system for 20 µL was as follows:reagents20µL reaction system2×qPCRMix10µLPrimerMixXµLProbeMixXµLTemplate4µLddH2OMake up to 20 µLNote: High Rox model: add 1 µL of 50×High Rox per 50 µL of reaction system; Low Rox model: add 1 µL of 50×High Rox per 500 µL of reaction system.Usually, better results can be obtained with a primer concentration of 0.2 µM, and 0.1-1.0 µM can be used as a reference for setting the range.The concentration of the probe used is related to the fluorescent quantitative PCR instrument used, the type of probe, and the type of fluorescent labeling substance, so please refer to the manual of the instrument or the specific requirements for the use of each fluorescent probe for the adjustment of the concentration during actual use.Prepare a sufficient amount of reaction system mixture as required. After the reaction system has been prepared and mixed thoroughly, add 16 µL per well to the reaction wells. Then add the prepared standard and diluted sample into the corresponding reaction wells, the volume of addition is 4µL/well. TE was added to the blank control tube, and the same amount of TE was added at 4 µL/well.It is recommended to use 20 µL for the reaction, if you need to perform a smaller system reaction, reduce the system components in equal proportion.4. qPCR reaction programThe following is an example of our GoldStar Probe Mixture reaction conditions, which should be improved and optimized according to the PCR product template, primer structure and target fragment size.movetemptimingcirculatepremutability95°C10min1denaturation95°C10sec55Annealing/Extension60°C30sec5Data analysis1. Standard curve productionThe standard curve was plotted with reference to the Excel sheet for data processing. The correlation coefficient R2 of the standard curve should not be lower than 0.98, and the slope should be between -3.1 and -3.6 when the Ct value is the vertical coordinate. If the parameters of the standard curve are unreasonable, it is recommended to repeat the experiment... Read More | Inquire |