| Description | Physical appearance: LiquidStorage buffer solution: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description1. This product is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 5000bp, Physical appearance: LiquidStorage buffer solution: GelRed dye, Stabilizer, 10mM Tris-HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product Description1. This product is composed of 9 linear double stranded DNA fragments, ranging in size from 100bp to 5000bp, including 100bp, 250bp, 500bp, 750bp, 1000bp, 1500bp, 2000bp, 3000bp, and 5000bp. 500bp and 1500bp are highlighted bands with concentrations 2.5 times higher than other bands, making them easier to observe after electrophoresis.2. In this product, the content of regular stripes is about 30ng, and the content of bright stripes is about 75ng.3. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis, making it easy to use.4. This product and its accompanying 5xLoading buffer already contain Gelred nucleic acid dye, which can be used in conjunction. After electrophoresis, it can be directly observed under UV without the need for subsequent staining treatment.5. This product is not suitable for polyacrylamide gel electrophoresis.6. Recommended concentration of electrophoresis buffer and agarose gel:1xTAE electrophoresis bufferAgarose concentration: 1.5%~2.0%7. Using this product system, no nucleic acid dye is needed in agarose gel.instructions1. Prepare agarose gel with appropriate concentration and without any nucleic acid dye.2. The concentration of gel has a great influence on DNA electrophoresis. The recommended concentration of agarose gel for this product is 1.5%~2.0%.3. It is recommended to use 1xTAE buffer with an electrophoresis voltage not exceeding 10v/cm.4. The commonly used 3.5mm sample well requires a recommended amount of 3-5 µ l for DNA markers, and the sample size should be appropriately increased for wide gel wells.5. Mix the sample to be tested with the matching 5xLoading buffer in a ratio of about 4:1, and then add it to the gel sampling hole.6. Electrophoretic to appropriate distance:Because Gelred is firmly bound to DNA, it can make full use of the length of gel, and the electrophoresis distance is longer, as long as the smallest segment does not run out of the gel, so as to facilitate the electrophoresis separation of small segments. Generally, the distance between the bromophenol blue indicator band and the edge of gel shall not be less than 1cm.7. After electrophoresis, observe the electrophoresis bands under ultraviolet light.8. The 5x loading buffer included in the product is used for sampling after mixing with the sample to be tested, and contains dual indicators of bromophenol blue and xylene blue.9. If there are a large number of samples that can be directly sampled and require electrophoresis detection, it is recommended to use Gelred gel method for detection without pre mixing samples, which can save a lot of experimental time. Product componentR751622Component100 T500TStorageR751622AGelred-prestained DNA Ladder (100-5000bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.R751622BGelred-prestained 5xLoading buffer 500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle... Read More | Inquire | Purity>95% (SDS-PAGE&HPLC) Endotoxin level<0.1 EU/µgFunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn Purity>95% (SDS-PAGE&HPLC) Endotoxin level<0.1 EU/µgFunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.Gal-1 is also engaged in many protein-protein interactions. Gal-1 plays a number of crucial roles in neuronal cell differentiation and survival in both the central and the peripheral nervous systems, and the establishment and maintenance of T-cell tolerance and homeostasis in vivo... Read More | Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells Purity>97% by SDS-PAGE and HPLC analyses.FunctionPlays an important role in the organization of the cytoskeleton (By similarity). Binds to and sequesters actin monomers (G actin) and therefore inhibits actin polymerization. Seraspenide inhibits the entry of hematopoeitic pluripotent stem cells into the S-phase... Read More | Purity> 97 % by SDS-PAGE and HPLC analyses.FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation whichPurity> 97 % by SDS-PAGE and HPLC analyses.FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase... Read More |