| Description | Product Description:1. This product consists of nine linear double-stranded DNA fragments with a size range of 50bp to 500bp, specifically 50bp, 100bp, 150bp, 200bp, 250bp, 300bp, 350bp, 400bp, and 500bp. The 250bp band is an intensified indicator band, with a concentration 2.5 times higher than Product Description:1. This product consists of nine linear double-stranded DNA fragments with a size range of 50bp to 500bp, specifically 50bp, 100bp, 150bp, 200bp, 250bp, 300bp, 350bp, 400bp, and 500bp. The 250bp band is an intensified indicator band, with a concentration 2.5 times higher than that of the other bands, facilitating observation after electrophoresis.2. In 5µl of this product, the content of the regular bands is approximately 30ng, while the content of the intensified band is about 75ng. 3. This product is already preserved in a 1x Loading Buffer and can be directly used for electrophoresis, offering convenience in use.4. Both this product and the accompanying 5x Loading Buffer contain the Gelred nucleic acid stain. When used together, after electrophoresis, the bands can be directly observed under ultraviolet light without the need for subsequent staining procedures.5. This product is not suitable for polyacrylamide gel electrophoresis.6. Recommended Electrophoresis Buffer and Agarose Gel Concentration: 1x TAE electrophoresis buffer Agarose concentration: 1.5% to 2.5%.7. Using this product system, there is no need to add any nucleic acid dye in agarose gel.Usage Instructions:1. Prepare an agarose gel of the appropriate concentration without any nucleic acid stains.2. The concentration of the agarose gel has a significant impact on DNA electrophoresis. The recommended agarose gel concentration for this product is 1.5% to 2.5%.3. It is suggested to use 1x TAE buffer for electrophoresis, with a voltage not exceeding 10v/cm.4. For common 3.5mm sample wells, the recommended volume of DNA marker is 3 to 5µl. For wider gel wells, the sample volume should be appropriately increased.5. Mix the samples to be tested with the accompanying 5x Loading Buffer at a ratio of approximately 4:1, and then load into the gel sample wells.6. Run the electrophoresis to an appropriate distance:Since Gelred binds firmly to DNA, it is possible to fully utilize the length of the gel and run a longer distance, as long as the smallest fragment does not run out of the gel, which is beneficial for the separation of small fragments. Generally, the bromophenol blue indicator band should be no less than 1cm away from the edge of the gel.7. After electrophoresis, observe the electrophoresis bands under a UV lamp.8. The 5x Loading Buffer included in the product is used for mixing with the samples to be tested before loading, and it contains both bromophenol blue and xylene cyan FF as dual indicators.9. If there are a large number of samples that can be directly loaded for electrophoresis testing, it is recommended to use the Gelred gel method for detection, without pre-mixing the samples, which can greatly save experimental time.Product componentG751633Component100 T500TStorageG751633AGelred-prestained DNA Ladder (50-500bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.G751633BGelred-prestained 5xLoading buffer 500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:Human B7 homolog 3 (B7-H3) is a member of the B7 family of immune proteins that provide signals for the regulation of immune responses. Other family members include B7-1, B7-2, B7-H1/PD-L1, B7-H2, and PD-L2. B7 family proteins are type I transmembrane immunoglobulin (Ig) superfamily members that contain extracellular Ig V‑like and Ig C‑like domains with a short cytoplasmic tail. Among the family members there is about 20 - 40% amino acid (aa) sequence identity. B7-H3 was initially reported to be a 316 aa type I transmembrane precursor protein that contained a signal sequence, an extracellular region with one V‑type and one C‑type Ig domain, a transmembrane segment and a short cytoplasmic tail. Subsequent studies have identified a second 110 kDa form whose precursor is 534 aa in length. Termed 4IgB7-H3 or B7-H3b, this molecule has two additional Ig-like domains (one V‑type and one C‑type) and shows a ubiquituous expression pattern. It would appear that the human 4Ig form is the principal, if not the only form of B7-H3. Its precursor contains a 26 aa signal sequence, a 435 aa extracellular region, a 31 aa transmembrane domain, and a 42 aa cytoplasmic tail. The four Ig-like domains alternate between V‑type and C‑type, and apparently are the consequence of a V‑C type tandem duplication. B7-H3b is expressed on dendritic cells as well as activated T, B and NK cells. The mouse gene differs from that of human in that it cannot code for four Ig-like domains; only a V‑type:C‑type pair. Human B7-H3b binding to an undefined receptor has shown to be inhibitory to NK cell illing and cytokine release. It also seems to be required for late stage osteoblast differentiation... Read More | Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T Human CCL18 is encoded by the CCL18 gene located on the chromosome 17. As also named MIP-4, it shares 61 % sequence identity to human MIP-1α. CCL18 is mainly expressed by lung and some lymphoid tissues like lymph nodes express CCL18 at low level. It is chemotactic for both activated (CD3+) T cells and nonactivated (CD14-) lymphocytes, but not for monocytes or granulocytes. Involved in B-cell migration into B-cell follicles in lymph nodes. CCL18 plays a role in both humoral and cell mediated immunity responses. Recombinant Human MIP-4/CCL18 is a 7.9kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.Purity>96% SDS-PAGEFunctionChemotactic factor that attracts lymphocytes but not monocytes or granulocytes. May be involved in B-cell migration into B-cell follicles in lymph nodes. Attracts naive T-lymphocytes toward dendritic cells and activated macrophages in lymph nodes, has chemotactic activity for naive T-cells, CD4+ and CD8+ T-cells and thus may play a role in both humoral and cell-mediated immunity responses... Read More | Purity>95% as determined by SDS-PAGE and Coomassie blue stainingFunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.Sequence Purity>95% as determined by SDS-PAGE and Coomassie blue stainingFunctionThe heparin-binding fibroblast growth factors play important roles in the regulation of cell survival, cell division, angiogenesis, cell differentiation and cell migration. They are potent mitogens in vitro.Sequence similaritiesBelongs to the heparin-binding growth factors family.Cellular localizationSecreted. Cytoplasm. Cytoplasm > cell cortex. Lacks a cleavable signal sequence. Within the cytoplasm, it is transported to the cell membrane and then secreted by a non-classical pathway that requires Cu(2+) ions and S100A13. Secreted in a complex with SYT1... Read More | BackgroundStreptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L,BackgroundStreptavidin is a tetrameric bacterial protein isolated from Streptomyces avidinii providing 4 high-affinity biotin binding sites. Streptavidin homo-tetramers have an extraordinarily high affinity for biotin. With a dissociation constant on the order of ≈10⁻¹⁴ mol/L, the binding of biotin to streptavidin is one of the strongest non-covalent interactions known in nature. Unlike egg-white avidin, which has a net positive charge at neutral pH and contains about 7% carbohydrate, streptavidin has almost no net charge at neutral pH, does not contain carbohydrate, and exhibits lower non-specific background. Streptavidin conjugates are widely used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids and other molecules. This FITC-streptavidin conjugate was prepared by highly purified Streptavidin and free FITC was removed. Streptavidin (FITC) is a useful second-step reagent for the indirect immunofluorescent staining of cells in combination with biotinylated primary antibodies for flow cytometric analysis. Excitation at 488nm light leads to a fluorescence emission maximum of 520 nm.Recommended Usage：Every lot of Streptavidin-FITC is tested by flow cytometry using biotinylated primary antibodies. From this testing it is recommended that between 0.02 and 0.25 µg of streptavidin be used per 106 cells in a 100 µl staining volume... Read More |