| Description | Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product IntroductionThis product is composed of 9 linear double stranded DNA fragments, stored in a 1xLoading Buffer, with dense bands that require high concentration gel and low voltage Storage buffer: 10mM Tris HCl, 5mM EDTA, 0.03% Bromophenol Blue, 0.03% Xylene Cyanol, 30% Glycerol, pH7.6Product IntroductionThis product is composed of 9 linear double stranded DNA fragments, stored in a 1xLoading Buffer, with dense bands that require high concentration gel and low voltage electrophoresis. It is suitable for accurately confirming the size of DNA fragments. The size range is 50-500bp, which are 50bp, 100bp, 150bp, 200bp, 250bp, 300bp, 350bp, 400bp, and 500bp respectively; 250 bp is the bright band, with a concentration 2.5 times that of other bands; In the 5 ul product, each strip contains about 40 ng, and the highlight strip contains about 100 ng.Usage suggestions1. It is recommended to sample 5 ul and increase the sample size appropriately for wide adhesive holes.2. It is recommended to use 2.5-3.5% Agarose or 5% PAGE gel, voltage 4-10 v/cm, and 0.5xTBE buffer for electrophoresis. Pay attention to replacing the electrophoresis buffer in time and using the newly prepared gel to achieve ideal results.3. Stain with EB or other nucleic acid dyes, and observe the electrophoretic bands under UV light.matters needing attention1. This product has been saved in a 1xLoading Buffer and can be directly used for electrophoresis. It is easy to use and the electrophoresis image is clear.2. When using agarose gel containing EB for electrophoretic detection, the bromophenol blue band can be electrophoretic to about 2/3 of the distance, otherwise the small segment will become dark because EB is separated from DNA.3. The accompanying 5xLoading buffer can be used for sample detection.5x Loading Buffer components:10 mM Tris-HCl，5 mM EDTA， pH7.6， 0.03% bromophenol blue, 0.03% xylene blue, 30% glycerol Product componentD745352Component100 T500TStorageD745352ADNA Ladder (50-500bp)500 µL5× 500 µL-20℃. Avoid freeze/thaw cycle.D745352B5xLoading buffer1 mL5× 1 mL-20℃. Avoid freeze/thaw cycle... Read More | Heme Oxygenase-1-IN-1 (Compound 2) is a heme oxygenase 1 ( HO-1 ) inhibitor with an IC 50 of 0.25 µMIC50& Target:IC 50 : 0.25 µM (HO-1) | Inquire | Purity>95% (SDS-PAGE&HPLC) Endotoxin level<0.1 EU/µgFunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn Purity>95% (SDS-PAGE&HPLC) Endotoxin level<0.1 EU/µgFunctionMay regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase.Gal-1 is also engaged in many protein-protein interactions. Gal-1 plays a number of crucial roles in neuronal cell differentiation and survival in both the central and the peripheral nervous systems, and the establishment and maintenance of T-cell tolerance and homeostasis in vivo... Read More | Keratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelialKeratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelial cells. Its mitotic activity is mainly manifested in keratinocytes, which can specifically promote the proliferation, migration and differentiation of epithelial cells. It is closely related to organ development, wound repair, tumor genesis and immune reconstruction.Activity definition: The ED50 value is less than 1.0 ng/ml, that is, the corresponding activity unit is greater than or equal to 1 x 10*6 units/mg, as determined by the proliferation method of cultured MCF-7 cells... Read More |