| Description | Albumin gene is mapped to human chromosome 4q13.3. It is synthesized as a preproalbumin with 609 amino acids in the liver. The mature albumin comprising 585 residues, is generated by the cleavage of signal peptide. Albumin is the major circulating protein in serum. It comprises three domains (I, II Albumin gene is mapped to human chromosome 4q13.3. It is synthesized as a preproalbumin with 609 amino acids in the liver. The mature albumin comprising 585 residues, is generated by the cleavage of signal peptide. Albumin is the major circulating protein in serum. It comprises three domains (I, II and III), each of which is subdivided into two subdomains A and B. A total of six ligand binding site is associated with albumin. Domain IIA and IIIA harbor small heterocyclic or aromatic compounds binding site. Long-chain fatty acid binding sites is present in IB and IIIB domains.Application:Albumin, glycated human has been used:as a component of serum-free medium for retinal microglia activationto stimulate immortalised proximal tubule epithelial (HK-2) cells for fibronectin productionas medium supplement in human retinal microvascular endothelial cells (HRMECs) cultures to test its effect on family with sequence similarity 18, member B (FAM18B) expressionNaturally occurring glycated albumin is a sensitive indicator of glycemic control by diabetic patients... Read More | Biochemical Test:SDS-PAGE (purity> 80%); Western blot with patient sample.Calculated Isoelectric Point:pH 8.83 | Inquire | Inquire | Ribonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleavesRibonuclease T1 is an endoribonuclease, highly specific for the cleavage of RNA or deaminated RNA between guanosine 3'-phosphate residues (or inosine 3'-phosphate) and the 5'-OH residues of adjacent nucleotides with the formation of the corresponding intermediate 2', 3'-cyclic phosphates. It cleaves single-stranded RNA releasing oligonucleotides from the guanosine 3'-phosphate termini. The enzyme has a molecular weight of 11 kDa. The optimum pH is 7.5. RNase T1 is inhibited by Ag+, Zn2+, Cu2+, and Hg2+ at 1 X 10-3 M. The stimulatory effects of both histidine and EDTA are attributed to chelation of contaminating inhibitor cations. The enzyme assay is essentially the method of Egami et al., Prog. in Nucleic Acid Res. and Molec. Biol., III, 59 (1964) based upon the release of acid soluble oligonucleotides following the digestion of yeast RNA.Ribonuclease T1 (RNase T1) from Aspergillus oryzae is used to digest denatured RNA prior to sequencing and is used for protein folding studies. ApplicationRibonuclease T1 has extensive applications in molecular cloning and DNA sequencing. Because of its specificity it has been a commonly used cleavage enzyme for the determination of structure, nearest neighbor frequencies, and RNA sequencing. The enzyme has further application in the preparation of nucleoside 2',3'-cyclic phosphates, the synthesis of oligonucleotides, and the removal of RNA from DNA preparations. The enzyme is also used as a non-mammalian source of RNase in various applications... Read More |