| Description | The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X The 6X His Peptide is commonly used for the competitive elution of His tag fusion proteins bound to His antibodies. It can be used to elute His tag fusion proteins from Anti-His Magnetic Beads and conventional Anti-His antibodies during immunoprecipitation.This product competitively elutes the 6X His fusion protein. The eluted protein sample does not contain the light and heavy chains of Anti-His antibody, thus effectively eliminating the interference of anibodies with downstream Western blot analysis.The amino acid sequence of 6X His Peptide is His-His-His-His-His-His (HHHHHH).The main parameters of 6X His Peptide are as followsPrecautions：The solution of this product should be aliquoted and stored at -20℃ or lower temperatures to avoid repeated freeze-thaw cycles.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. For 25mg provided in powder form, centrifuge at 8,000-12,000×g for 10-30 seconds before opening to collect the powder at the bottom of the tube. Dissolve it in TBS by gently pipetting or shaking. Do not vortex vigorously to avoid denaturation and inactivation of the peptide.2. P9811-1mg and P9811-5mg can be directly used for preparing elution buffer. 3. Competitive elution with the 6X His Peptide: This elution method is non-denaturing, with high elution efficiency, and the eluted sample does not contain the light and heavy chains of His antibodies.a. Preparation of 6X His tag elution buffer: Based on the required amount of elution buffer, dilute an appropriate amount of 6X His Peptide stock solution with TBS to a final concentration of 150µg/ml.b. Add 100µl of 6X His tag elution buffer (150µg/ml) to each immunoprecipitated sample, and incubate on ice or at 4℃ for 30 minutes to 2 hours with shaking. In order to improve the elution efficiency, the incubation time can be extended or the elution can be repeated. The volume of the 6X His tag elution buffer is generally 5 times that of the bead or gel suspension.c. Centrifuge at 6000×g for 30 seconds at 4℃, and carefully transfer the supernatant to a new tube. The supernatant contains the 6X His fusion protein and its protein complexes. Be careful not to touch the resin when taking the supernatant. 4. Store the eluted 6X His fusion protein at 4℃ for immediate use, or at -20℃ for long-term storage... Read More | Inquire | Product DescriptionEndo F1 cleaves Asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 fold. Endoglycosidase F1 will hydrolyze sulfate containing high-mannose chains. It cleaves between the two N-acetylglucosamine residues in the Product DescriptionEndo F1 cleaves Asparagine-linked high mannose and some hybrid oligosaccharides. Core fucosylation reduces the activity by 50 fold. Endoglycosidase F1 will hydrolyze sulfate containing high-mannose chains. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.Molecular weight 32,000 daltonsContents60 µl aliquot of enzyme (1 U) in 20 mM Tris-HCl, pH 7.5Included with 20 µL and 60 µL pack sizes:5x Reaction Buffer – 250 mM sodium phosphate, pH 5.5Specific ActivityDefined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured Ribonuclease B (RNase B) in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved RNase B migrates faster).FormulationThe enzyme is provided as a sterile-filtered solution in 20 mM Tris-HCl, pH 7.5StabilitySeveral days exposure to ambient temperatures will not reduce activity. Stable at least 12 months when stored properly. SpecificityEndo F1 cleaves Asparagine-linked high mannose or hybrid oligosaccharides. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Quality & PurityEndo F1 is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases.Directions for use1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water.2. Add 10 µl 5x Reaction Buffer 5.53. Add 2.0 µl of Endo F1 to the reaction. Incubate 1 hour or more at 37°C.Monitor cleavage by SDS-PAGE... Read More | Keratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelialKeratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelial cells. Its mitotic activity is mainly manifested in keratinocytes, which can specifically promote the proliferation, migration and differentiation of epithelial cells. It is closely related to organ development, wound repair, tumor genesis and immune reconstruction.Activity definition: The ED50 value is less than 1.0 ng/ml, that is, the corresponding activity unit is greater than or equal to 1 x 10*6 units/mg, as determined by the proliferation method of cultured MCF-7 cells... Read More | Inquire |