| Description | EnzymoPure™Q M-MLV Reverse Transcriptase uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand with high fidelity, sensitivity, and specificity. It is a modified, optimized reverse transcriptase particularly suitable for qPCR analysis. The EnzymoPure™Q M-MLV Reverse Transcriptase uses single-stranded RNA or DNA in the presence of a primer to synthesize a complementary DNA strand with high fidelity, sensitivity, and specificity. It is a modified, optimized reverse transcriptase particularly suitable for qPCR analysis. The EnzymoPure™Q M-MLV Reverse Transcriptase contains RNase H enzyme activity that can specifically degrade the RNA strand of an RNA-DNA hybrid, enabling the subsequent synthesis of second-strand cDNA.EnzymoPure™Q M-MLV Reverse Transcriptase, a commonly used high-quality reverse transcriptase, is widely used in the synthesis of first-strand cDNA using total RNA or mRNA templates, and is particularly suitable for qPCR and one-step qRT-PCR analysis. This product is also applicable for conventional PCR, synthesis of second-strand cDNA and construction of cDNA library, and cloning of target genes. It can also be used for DNA probe labeling with fluorescence, biotin, digoxin, or isotope through reverse transcription reaction, or for RNA studies by primer extension.Please refer to Figure 1 for the qPCR analysis of the GADPH gene in HEK293T cells using the cDNA templates obtained by the EnzymoPure™Q M-MLV reverse transcriptase.Figure 1Source：Recombinant reverse transcriptase expressed in E. coli.Definition of enzyme activity unit: One unit is defined as the amount of enzyme that incorporates 1 nmol of dTTP into acid-precipitable material in 10min at 37℃ using poly(A)•oligo(dT)12-18 as template-primer. Reaction system: 50mM Tris-HCl (pH8.3), 75mM KCl, 3mM MgCl2, 10mM DTT, 0.5mM [3H]-dTTP, and 0.4mM polyA•oligo(dT)12-18.Purity: This product is free from DNA endonuclease, exonuclease, phosphatase, and RNase, and can meet the requirements of conventional reverse transcription.Storage buffer: 20mM Tris-HCl (pH7.5), 150mM NaCl, 0.1mM EDTA, 1mM DTT, 0.01%(v/v) NP-40, and 50%(v/v) glycerolInactivation or inhibition：RT Q M-MLV Reverse Transcriptase can be inactivated by incubation at 80℃ for 10 minutes, or inhibited by EDTA and EGTA chelators, inorganic phosphates or pyrophosphates, and polyamine.The concentration of M-MLV is 200U/µl. When 20µl of reverse transcription reaction volume is used, D7188S, D7188M, and D7188L are sufficient for 50, 250, and 1000 reactions, respectively. provides a variety of reverse transcriptase. To select the best one for your needs, please refer to the following webpage:http://www.aladdin-e.com/support/reversetranscriptase.htmPrecautions：Please refer to the instructions for reverse transcription of RNAs with high GC content.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. First-strand cDNA synthesisa. Set up the following reaction on ice or at room temperature. RNase Inhibitor and dNTP mix can be purchased from SYBR Green qPCR Mix (2X), SYBR Green qPCR Mix (2X, Low ROX), and SYBR Green qPCR Mix (2X, High ROX) are for conventional qPCR analysis with SYBR Green fluorescent dye. Probe qPCR Mix (2X), Probe qPCR Mix (2X, Low ROX), and Probe qPCR Mix (2X, High ROX) are for qPCR analysis with Taqman Probe.b. Perform qPCR analysis following the instructions of the corresponding qPCR kit.3. For other applications such as primer extension and probe labeling, please refer to references related to M-MLV reverse transcriptase.FAQ:1. The reverse transcription product of total RNA is invisible after electrophoresis.It is a normal phenomenon, because the amount of RNA template is low, and the amount of reverse transcription products in different sizes is even lower. 2. No specific product is amplified from the reverse transcription product.a. To exclude the problem of PCR reaction system or reverse transcription product, use gene-specific primers to amplify internal reference genes, such as actin and GAPDH. If reference genes can be amplified, but not the target gene, it indicates primers of the target gene are not well designed. b. Template RNA may be degraded. The integrity of total RNA can be checked by agarose gel or on-chip electrophoresis. Intact total RNA exhibits sharp, clear 28S and 18S rRNA bands, and the 28S rRNA band should be approximately twice as intense as the 18S rRNA band. A ratio less than 2 indicates the degradation of total RNA and new total RNA should be prepared. c. RNA samples may contain some components that inhibit the activity of reverse transcriptase. Those contaminants include phenol, SDS, EDTA, guanidine salts, phosphoric acid, pyrophosphoric acid, polyamine, spermidine, etc, which can be removed effectively by column purification or precipitation, washing, and redissolution. Total RNA extracted by Zol or Trizol produced by can fully meet the requirements of reverse transcription.d. Insufficient templates for reverse transcription. When DNase I is used to remove the residual DNA in the RNA sample prior to reverse transcription and is subjected to heat-inactivation, EDTA should be added to the RNA sample at a final concentration of 2.5mM to protect RNA from degradation under high temperature. Additionally, to amplify a specific gene, it is necessary to extract RNA from tissues in which the target gene is highly expressed. e. An inappropriate primer is used for reverse transcription. Random hexamer instead of Oligo(dT)18 should be used for the reverse transcription of bacterial total RNA which does not have poly(A) tails. Gene-specific primers used for reverse transcription must be well-designed... Read More | Cardiolipin is a unique phospholipid present in the inner mitochondrial membrane, which makes up to 20% of total lipids. It is a non-bilayer anionic phospholipid, which has four acyl chains and small headgroupHeart CA has been used as a standard stock solution for its quantitative analysis using Cardiolipin is a unique phospholipid present in the inner mitochondrial membrane, which makes up to 20% of total lipids. It is a non-bilayer anionic phospholipid, which has four acyl chains and small headgroupHeart CA has been used as a standard stock solution for its quantitative analysis using liquid chromatography?mass spectrometry (LC-MS)/MS. It has also been used for liposome preparation... Read More | Inorganic pyrophosphates are inevitably produced in the process of mRNA transcription in vitro. These substances have a great inhibitory effect on transcription. Inorganic pyrophosphatase (PPase) can hydrolyze the inorganic pyrophosphates produced in nucleic acid amplification experiments, promote Inorganic pyrophosphates are inevitably produced in the process of mRNA transcription in vitro. These substances have a great inhibitory effect on transcription. Inorganic pyrophosphatase (PPase) can hydrolyze the inorganic pyrophosphates produced in nucleic acid amplification experiments, promote the shift of reaction equilibrium to the product generation end, and increase the amount of products.The molecular weight of PPase (pyrophosphatase, inorganic, inorganic pyrophosphatase) is about 63kd, which can catalyze the hydrolysis of inorganic pyrophosphate to produce orthophosphate: P2O74_+H2O+PPase→2HPO42_. In the nucleic acid amplification experiment, PPase can hydrolyze the inorganic pyrophosphate generated with the reaction to avoid its inhibition on the reaction system. The removal of pyrophosphate can shift the reaction equilibrium to the product generation end.This product is a GMP level recombinant inorganic pyrophosphatase (yeast source) expressed by large-scale fermentation of E. coli. It is produced with raw and auxiliary materials of medicinal specifications, and the host protein residue and nucleic acid residue are strictly controlled. The product production and quality management procedures in line with GMP specifications ensure that the production process and all raw and auxiliary materials can be traced.Quality requirements project standard appearance Clear liquid Visible foreign matter Compliance with regulations PH value 7.5±8.5 activity 98U/ml-102U/ml purity ≥95% Endonuclease residues Degradation of substrate shall not exceed 10% Exonuclease residues Degradation of substrate shall not exceed 10% RNase residue Degradation of substrate shall not exceed 10% Bacterial endotoxin content ≤10EU/ml Exogenous DNA residue ≤100pg/mg Host protein residue ≤50ppm Mycoplasma detection negative Heavy metal residues ≤10ppm Follow the following specifications1. ISO 9001:2015, certified facility。2. GMP appendix - cell therapy products State Drug Administration.3. general introduction to human gene therapy - Chinese Pharmacopoeia 2020, National Pharmacopoeia Committee.4. USP chapter <1043>, adjuvant materials for cell, gene, and tissue engineered products.5. USP chapter <92>, growth factors and cytokines used in cell therapy manufacturing.6. Ph. Eur. General chapter 5.2.12, raw materials of biological origin for the production of cell-based and gene therapy medical products.Product features1. hydrolyze inorganic pyrophosphate.2. DNA synthesis: significantly enhance DNA replication ability.3. RNA synthesis: increase RNA production in in vitro transcription reaction.4. The optimal reaction temperature is 25℃, and the enzyme can be inactivated at 65℃ for 10min.Product usage1. optimize RNA transcription: improve the RNA yield of in vitro transcription reaction.2. remove PPI contamination from reagents for SNP genotyping by pyrophosphate assay.3. promote the synthesis of protein, RNA and DNA.4. catalyze the reaction of PPI + H2O → 2pi.5. ssr-pcr optimization：Improve efficiency and increase DNA production.Activity definitionCatalytic inorganic pyrophosphate formation 1 per minute under standard reaction conditions µ The amount of enzyme required for mol phosphate was defined as 1 active unit.Preservation system20 mM Tris-HCl; 100 mM NaCl; 1 mM DTT; 0.1 mM EDTA; 50% (v/v) Glycerol; pH 8.0。 Storage temperature-20±5 ℃。Matters needing attention1. the enzyme has activity in various reaction buffers. Generally, the enzyme can be directly added in HDA, lamp and other experiments.2. the dosage of the enzyme needs to be optimized in different experiments, usually adjusted at the concentration of 0.05~1u/ml.3. the optimum reaction temperature of the enzyme was 25 ℃, and it was active at 16~37 ℃, and the enzyme could be inactivated at 65 ℃ for 10min.4. cofactor: mg2+ is necessary for enzyme activity... Read More | Purity>95% (SDS-PAGE) Endotoxin level<1.0 EU/µgFunctionInhibits the synthesis of a number of cytokines, including IFN-gamma, IL-2, IL-3, TNF and GM-CSF produced by activated macrophages and by helper T-cells | Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa Trypsin is a pancreatic serine protease with substrate specificity based upon positively charged lysine and arginine side chains. It is derived from a 34 kDa inactive precursor zymogen, trypsinogen, after enzymatic removal of an N-terminal 6-amino acid leader sequence resulting in the 23.8 kDa trypsin molecule. The optimum pH is 8.0. Trypsin is inhibited by organophosphorus compounds such as diisopropylfluorophosphate and natural inhibitors from pancreas. Soybean, lima bean, and egg white are also sources of natural inhibitors. Trypsin cleaves amide and ester bonds of Arg and Lys. The Aladdin Sequencing Grade Trypsin has been further purified to remove trace contaminating proteases and autolysis products which could interfere in trypsin digestion experiments, and exhibits a single band on PAGE.Trypsin is a serine protease used to hydrolyze proteins. Trypsin from bovine pancreas has a molecular weight of 23.8 kDa. Trypsins are used for the re-suspension of cells during cell culture and in proteomics research for the digestion of various proteins... Read More |