| Description | SpeciesGene IDAccessionECSourceLengthMWTag2019-nCoV43740578YP_009725301.13.4.22.69E. coli306aa~33.8kDa-About this proteinName2019-nCoV Main ProteaseSynonyms2019-nCoV Main Protease (Mpro)/ 3C-like Protease (3CLpro), Mpro/3CLpro, 2019-nCoV 3C-like Proteinase, main proteinase, 3C-like proteinase (NSP5)SpeciesGene IDAccessionECSourceLengthMWTag2019-nCoV43740578YP_009725301.13.4.22.69E. coli306aa~33.8kDa-About this proteinName2019-nCoV Main ProteaseSynonyms2019-nCoV Main Protease (Mpro)/ 3C-like Protease (3CLpro), Mpro/3CLpro, 2019-nCoV 3C-like Proteinase, main proteinase, 3C-like proteinase (NSP5), Coronavirus endopeptidase C30, chymotrypsin-like protease, coronavirus 3C-like protease, coronavirus M(pro), coronavirus main proteaseBackground2019-nCoV is a positive-sense single-stranded RNA virus and has high homology with SARS-CoV and MERS-CoV. After infecting host cells, the coronaviral ORF1a/b RNA is first translated with the help of host cells into two polyprotein precursors (PP1a and PP1ab) which are then cleaved by Mpro and papain like proteases to produce multiple nonstructural proteins. These nonstructural proteins are involved in the production of viral subgene RNA and four structural proteins (envelope/E protein, membrane/M protein, spike/S protein and nucleocapsid/N protein), which in turn complete the reproduction and release of the progeny virus. Since the Mpro protease plays a vital role in the life cycle of the virus and there is no homologous protein in the human body, the Mpro main protease is an ideal target for developing antiviral drugs. Meanwhile because Mpro is highly conservative among beta coronaviruses, the Mpro inhibitors selected have a broad-spectrum anti-coronavirus ability and may even be used in curing other animal diseases such as porcine coronavirus.Aladdin's 2019-nCoV Main Protease is purified using the PerfectProtein™ Platform developed by aladdin. It is recombinantly expressed in E. coli without any additional tags or amino acids, ensuring complete identity with the natural Main Protease in 2019-nCoV virus.s2019-nCoV inhibitor screening; enzyme activity or structural studiesPhysical AppearanceliquidBiological Activity≥300U/g or 300µmol/min/gUnit DefinitionOne unit is defined as the amount of enzyme that catalyzes the production of 1µmol of MCA-AVLQ per minute at 37℃ under pH7.0. Concentration~1mg/mlPurity≥ 95% by SDS-PAGE.Formulation500mM Tris, 150mM NaCl, 1mM EDTA, 50% Glycerol, pH7.3Recommended UsageFor inhibitor screening, add 0.2-1µg of this product generally in a reaction volume of 200µl.Amino Acid SequenceSGFRKMAFPS GKVEGCMVQV TCGTTTLNGL WLDDVVYCPR HVICTSEDML NPNYEDLLIR KSNHNFLVQA GNVQLRVIGH SMQNCVLKLK VDTANPKTPK YKFVRIQPGQ TFSVLACYNG SPSGVYQCAM RPNFTIKGSF LNGSCGSVGF NIDYDCVSFC YMHHMELPTG VHAGTDLEGN FYGPFVDRQT AQAAGTDTTI TVNVLAWLYA AVINGDRWFL NRFTTTLNDF NLVAMKYNYE PLTQDHVDIL GPLSAQTGIA VLDMCASLKE LLQNGMNGRT ILGSALLEDE FTPFDVVRQC SGVTFQNotePrecautions：Before opening the tube, centrifuge briefly to collect liquid at the bottom of the tube.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：1. For the usage of this product, please refer to the manual of ’s Coronavirus Mpro/3CLpro Activity Fluorometric Assay Kit or other relevant literature, depending on the experimental purposes.2. Please refer to Figure 1 for the performance of this product in cleaving the CoV Main Protease Fluorogenic Substrate, MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2, into a fluorescent product with Ex/Em of 325/393nm.Figure 1. Digestion of MCA-AVLQSGFR-Lys(Dnp)-Lys-NH2 by different amounts of 2019-nCoV Main Protease (Mpro/3CLpro) (, P2320). This figure is for reference only, which may vary due to different experimental conditions... Read More | Inquire | TEV Protease is the 241 amino acid (aa), 27 kDa catalytic domain of the nuclear inclusion a (NIa) protein encoded by the potyvirus, tobacco etch virus (TEV). It may be used in biotechnology to cleave affinity tags from recombinant proteins, either co-translationally orin vitrofollowing purification.TEV Protease is the 241 amino acid (aa), 27 kDa catalytic domain of the nuclear inclusion a (NIa) protein encoded by the potyvirus, tobacco etch virus (TEV). It may be used in biotechnology to cleave affinity tags from recombinant proteins, either co-translationally orin vitrofollowing purification. Its high specificity and activity at a wide range of pH and ionic strength make TEV Protease more versatile than many other proteases used for the same purpose. Unlike factor Xa, enteropeptidase or thrombin, TEV Protease has not been found to cleave at unintended sites, even when present at a high concentration. TEV Protease is a 3C-type protease that cleaves substrates with a consensus sequence of ENLYFQG. Cleavage occurs between Q and G. Since the final aa remains on the cleaved protein where it could potentially affect structure or function, substitution of a variety of aa have been tested. In order of efficiency, S, A, M, Y, D, N, E, K or L may be effectively used in place of G. Several of the remaining aa may also vary, giving a final consensus sequence of ExxYF(M)Q(E)/G(S, A or others) where aa in parenthesis are alternatives and x is any aa. The autocatalytic site of NIa at S2256 has been mutated to an N for improved stability of the protease.Tobacco Etch Virus Protease is a highly site-specific cysteine protease that is found in the tags from fusion proteins. The optimal temperature for cleavage is 30°C. It is recommended that the cleavage for each fusion protein be optimized by varying the amount of recombinant viral TEV protease, reaction time, or incubation temperature. It can be removed by Ni2+ affinity resin... Read More | Purity≥97% (SDS-PAGE) Endotoxin level<1.0 EU/µgFunctionSupports IL-2 independent and IL-4 independent growth of helper T-cells | Inquire |