| Description | Product Description：Active blocker, recommended for IgM detection products.IgM antibody detection is an effective diagnostic method to determine the initial or early infection of pathogens. Rheumatoid factor (RF) and specific IgG antibodies in serum samples may affect the specificity and Product Description：Active blocker, recommended for IgM detection products.IgM antibody detection is an effective diagnostic method to determine the initial or early infection of pathogens. Rheumatoid factor (RF) and specific IgG antibodies in serum samples may affect the specificity and sensitivity of detection reagents, resulting in erroneous results. Using IgG/RF adsorbent to pretreat samples to remove the above-mentioned substances that may interfere is the simplest and fastest way to improve detection specificity, sensitivity and stability.Goat anti-human IgG (Fc) antibody (0.01M PBS, pH7.4), purified by antigen affinity chromatography, with a purity ≥ 95%, filtered through 0.2 µm, without adding preservatives.Filtration: 0.2umNotes：Long-term storage should be ≤-20°C. The product can be stored at 2°C-8°C for a short period of time without opening, and the storage time should not exceed 7 days. Because this product does not add any preservatives, if it is opened and used, it is not recommended to store it at 2-8°C. For long-term storage at ℃, it should be subpackaged and stored at ≤-20℃.Technical background:Specific IgM detection is a serological diagnostic method for judging the initial or early infection of pathogens. Rheumatoid factor (RF) and specific IgG antibodies in serum samples may affect the specificity and sensitivity of detection reagents, resulting in erroneous results. It is the simplest and fastest way to improve the specificity, sensitivity and stability of detection by using IgG/RF adsorbent, a special blocking agent for IgM detection, to pretreat the sample to remove the above-mentioned substances that may interfere.Action principle:After the adsorbent is in contact with the sample, the IgG antibody in the serum reacts with the adsorbent to form an IgG complex, which prevents it from competing with the IgM to be tested for antigen binding, and improves the detection sensitivity. At the same time, the rheumatoid factor (RF) that may exist in the sample will be eliminated. The IgG complex is adsorbed to remove its interference to the detection process.Method of application:1. Immunochromatographic productsThe adsorbent can be added to the sample processing pad at a certain concentration, and the concentration used is determined through a gradient test according to the amount of sample used.2. ELISA products2.1. The sample can be pretreated by adding the adsorbent to the sample diluent.2.2. Determine the content of the adsorbent in the sample diluent according to the sample usage and dilution of the corresponding product, and the most suitable concentration should be determined through a gradient test.2.3. The pretreatment time is between 5 and 30 minutes and should be determined experimentally.2.4. The pretreated sample may appear turbid due to IgG complexes. It can be centrifuged at low speed for 2-3 minutes to remove the precipitate and take the supernatant for detection. If it is verified that the turbid sample has no effect on the experimental results, it can be directly tested.2.5. The effect of adding adsorbents on the reaction system in the sample dilution should be fully considered to ensure that the active ingredients in the sample dilution are not affected by the adsorbent.3. Other IgM detection products The use method and concentration of the adsorbent should be determined according to the corresponding technical route and detection method. An inappropriate concentration of the adsorbent may cause precipitation, and the most suitable concentration should be selected through experiments... Read More | Inquire | Purity>95% by SDS-PAGE and HPLC analyses.FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6Purity>95% by SDS-PAGE and HPLC analyses.FunctionLigand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation... Read More | Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and over-expressed in several human tumors. The mesothelin gene encodes a ~70 kDa Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： Mesothelin (MSLN), also known as CAK1 and ERC, is a glycosylated cell-surface antigen present on normal mesothelial cells and over-expressed in several human tumors. The mesothelin gene encodes a ~70 kDa precursor protein that is cleaved at a dibasic proteolytic site into a 40 kDa membrane-bound protein termed MSLN and a 31 kDa shed fragment called megakaryocyte-potentiating factor (MPF) that is released from the cell. Cleaved, human MSLN remains attached to the cell surface via a GPI linkage and shares 58% amino acid sequence identity with mouse and rat MSLN. In human, alternate splicing generates additional MSLN isoforms that have either an eight amino acid insertion following Ser408 or a substituted C‑terminal region with no GPI anchor. Mesothelin is normally expressed on mesothelial cells in the pleura, pericardium, and peritoneum as well as in the developing and postnatal pancreas. It is up‑regulated in mesotheliomas and a range of carcinomas and adenomas. Mesothelin promotes tumor cell proliferation, migration, anchorage-independent growth, and tumor progression. It is co‑expressed with the tumor antigen CA125/MUC16 on advanced ovarian adenocarcinomas and interacts with this molecule to support cell adhesion. A soluble form of Mesothelin is released from tumor cells into the serum or tissue effusions... Read More | Purity>98% SDS-PAGE. purified using conventional chromatography techniques.FunctionChemotactic activity for lymphocytes but not for monocytes or neutrophils.Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the Purity>98% SDS-PAGE. purified using conventional chromatography techniques.FunctionChemotactic activity for lymphocytes but not for monocytes or neutrophils.Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1. Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60 % amino acid sequence homology with the murine and rat protein... Read More |