| Description | Product Description：Active blocker, recommended for IgM detection products.IgM antibody detection is an effective diagnostic method to determine the initial or early infection of pathogens. Rheumatoid factor (RF) and specific IgG antibodies in serum samples may affect the specificity and Product Description：Active blocker, recommended for IgM detection products.IgM antibody detection is an effective diagnostic method to determine the initial or early infection of pathogens. Rheumatoid factor (RF) and specific IgG antibodies in serum samples may affect the specificity and sensitivity of detection reagents, resulting in erroneous results. Using IgG/RF adsorbent to pretreat samples to remove the above-mentioned substances that may interfere is the simplest and fastest way to improve detection specificity, sensitivity and stability.Goat anti-human IgG (Fc) antibody (0.01M PBS, pH7.4), purified by antigen affinity chromatography, with a purity ≥ 95%, filtered through 0.2 µm, without adding preservatives.Filtration: 0.2umNotes：Long-term storage should be ≤-20°C. The product can be stored at 2°C-8°C for a short period of time without opening, and the storage time should not exceed 7 days. Because this product does not add any preservatives, if it is opened and used, it is not recommended to store it at 2-8°C. For long-term storage at ℃, it should be subpackaged and stored at ≤-20℃.Technical background:Specific IgM detection is a serological diagnostic method for judging the initial or early infection of pathogens. Rheumatoid factor (RF) and specific IgG antibodies in serum samples may affect the specificity and sensitivity of detection reagents, resulting in erroneous results. It is the simplest and fastest way to improve the specificity, sensitivity and stability of detection by using IgG/RF adsorbent, a special blocking agent for IgM detection, to pretreat the sample to remove the above-mentioned substances that may interfere.Action principle:After the adsorbent is in contact with the sample, the IgG antibody in the serum reacts with the adsorbent to form an IgG complex, which prevents it from competing with the IgM to be tested for antigen binding, and improves the detection sensitivity. At the same time, the rheumatoid factor (RF) that may exist in the sample will be eliminated. The IgG complex is adsorbed to remove its interference to the detection process.Method of application:1. Immunochromatographic productsThe adsorbent can be added to the sample processing pad at a certain concentration, and the concentration used is determined through a gradient test according to the amount of sample used.2. ELISA products2.1. The sample can be pretreated by adding the adsorbent to the sample diluent.2.2. Determine the content of the adsorbent in the sample diluent according to the sample usage and dilution of the corresponding product, and the most suitable concentration should be determined through a gradient test.2.3. The pretreatment time is between 5 and 30 minutes and should be determined experimentally.2.4. The pretreated sample may appear turbid due to IgG complexes. It can be centrifuged at low speed for 2-3 minutes to remove the precipitate and take the supernatant for detection. If it is verified that the turbid sample has no effect on the experimental results, it can be directly tested.2.5. The effect of adding adsorbents on the reaction system in the sample dilution should be fully considered to ensure that the active ingredients in the sample dilution are not affected by the adsorbent.3. Other IgM detection products The use method and concentration of the adsorbent should be determined according to the corresponding technical route and detection method. An inappropriate concentration of the adsorbent may cause precipitation, and the most suitable concentration should be selected through experiments... Read More | Inquire | Inquire | Usually used industrially for the resolution of chiral compounds and the transesterification production of biodiesel | This reagent kit is based on TRIzon's improved columnar total RNA extraction kit. This product can be extracted from animal groupsExtract total RNA from samples such as textiles, plant materials, various microorganisms, and cultured cells. Firstly, the cracking solution is fully cracked This reagent kit is based on TRIzon's improved columnar total RNA extraction kit. This product can be extracted from animal groupsExtract total RNA from samples such as textiles, plant materials, various microorganisms, and cultured cells. Firstly, the cracking solution is fully cracked andHomogenized samples, in their unique high salt state, RNA specifically binds to silicon matrix membranes, greatly reducingEffectively removing organic solvent contamination while removing protein contamination, resulting in higher purity and quality of RNA. bookThe product can quickly extract total RNA from various cells or tissues, and can process 30-50 mg of tissue or 5 × 10 ⁶ cells each time,Can handle multiple different samples simultaneously. If it is an RNA experiment that is very sensitive to trace amounts of DNA, the residual DNA can be utilizedUsing DNase without RNase for digestion and removal on the column, the extracted RNA can be directly applied to RT-PCR Experiments such as Northern Blot, Dot Blot, and in vitro translation. U665516 Component 50 T Storage U665516A DNase I 1000 U -20℃. Avoid freeze/thaw cycle. U665516B 10×Reaction Buffer 1000 µL -20℃. Avoid freeze/thaw cycle. U665516C TRIzon Reagent 60 mL 2-8℃. Protect from light. U665516D TRIzon PaI™ 10 mL 2-8℃. Protect from light. U665516E Buffer RW1 40 mL RT U665516F Buffer RW2 (concentrate) 11 mL RT U665516G RNase-Free Water 10 mL RT U665516H Spin Columns RM with Collection Tubes 50 sets RT U665516I RNase-Free Centrifuge Tubes (1.5 mL) 50 EA RTPreparation and important precautions before the experiment:1.To prevent RNase pollution, attention should be paid to the following aspects:1) RNase's plastic products and gun heads to avoid cross contamination.2) Prepare the solution using water without RNase.3) Operators should wear disposable masks and gloves, and change gloves frequently during the experiment.2. The sample should avoid repeated freezing and thawing, otherwise it will affect the yield and quality of RNA extraction.3. If TRIzon Reagent is found to have precipitates before use, it can be dissolved in a water bath at 56 ℃ for a few minutes.Before the first use, anhydrous ethanol should be added to Buffer RW2 according to the instructions on the reagent bottle label.5. All centrifugation steps should be carried out at room temperature unless otherwise specified, and all operation steps should be carried out quickly.Usage：1. Sample processing1a. Organization: 30-50 mg of tissue is thoroughly ground in liquid nitrogen and 1 mL of TRIzon Reagent is added, or 1 mL of TRIzon Reagent is added to the tissue sample and homogenized. Attention: The sample volume should not exceed 10% of the volume of TRIzon Reagent.2a. Single layer cell culture: Remove the culture medium and add an appropriate amount every 10 cm ² Add 1 mL of TRIzon Reagent.3a. Cell suspension: Collect cells by centrifugation. Add 1 mL of TRIzon Reagent to every 5 × 10 µ m cell.2. After adding TRIzon Reagent, repeatedly blow a few times to fully crack the sample. Leave at room temperature for 5 minutes to completely separate the protein nucleic acid complex.3. Add 200 to every 1 mL of TRIzon Reagent µ LTRIzon PaI ™， Cover the tube tightly, vigorously shake for 15 seconds, and let it sit at room temperature for 2 minutes.4. Centrifuge at 4 ℃ 12000 rpm (~13400 × g) for 10 minutes. At this time, the sample is divided into three layers: the red organic phase, the middle layer, and the upper colorless aqueous phase. RNA is mainly in the upper aqueous phase. Move the upper aqueous phase to a new RNase Free centrifuge tube (provided).5. Add an equal volume of 70% ethanol (prepared without RNase water) to the obtained aqueous solution, invert and mix well.6. Add all the solutions obtained in the previous step to the spin columns RM that have been loaded into the collection tube. If the solution cannot be added at once, it can be transferred in multiple batches. Centrifuge at 12000 rpm for 20 seconds, discard the waste liquid in the collection tube, and place the adsorption column back into the collection tube.7. Add 350 to the adsorption column µ L Buffer RW1, centrifuge at 12000 rpm for 20 seconds, discard the waste liquid in the collection tube, and place the adsorption column back into the collection tube.8. Preparation of DNase I mixture: Take 52 µ LRNase Free Water, add 8 to it µ L 10 x Reaction Buffer and 20 µ L DNase I (1 U/ µ L) Mix well and prepare to a final volume of 80 µ The reaction solution of L.9. Directly add 80 µ L DNase I mixture to the adsorption column and incubate at 20-30 ℃ for 15 minutes.10. Add 350 to the adsorption column µ L Buffer RW1, centrifuge at 12000 rpm for 1 minute, discard the waste liquid, and place the adsorption column back into the recovery manifold.11. Add 500 to the adsorption column µ L Buffer RW2 (check if anhydrous ethanol has been added before use), centrifuge at 12000 rpm for 20 seconds, discard the waste liquid in the collection tube, and place the adsorption column back into the collection tube.12. Repeat step 11.Centrifuge at 12000 rpm for 2 minutes and discard the waste liquid from the collection tube. Place the adsorption column at room temperature for a few minutes and thoroughly air dry. Attention: The purpose of this step is to remove residual ethanol from the adsorption column, which will affect subsequent enzymatic reactions (enzyme digestion,. )PCR, etc.14. Place the adsorption column in a new RNase free centrifuge tube and add 30-50 to the middle of the adsorption column µ Place RNase Free Water at room temperature for 1 minute, centrifuge at 12000 rpm for 1 minute, collect RNA solution, and store RNA at -70 ℃ to prevent degradation.Attention:1) The volume of RNase Free Water should not be less than 30 µ L. Small volume affects the recovery rate.2) If you want to increase RNA production, you can use 30-50 µ Repeat step 14 for the new RNase Free Water.3) If you want to increase the RNA concentration, you can add the obtained solution back to the adsorption column and repeat step 14... Read More |