| Description | ApplicationFor the detection of lactic acid content.Enzymatic propertiesSource: MicroorganismEnzymology Committee Number: EC 1.1.3.2Molecular weight: 42 kDa (SDS-PAGE)Isoelectric point: pH 4.6Km value: 7.5 × 10-4 M (L-Lactate)Inhibitors: Hg²⁺, Pb²⁺ Optimum pH: 6.0-7.0 ApplicationFor the detection of lactic acid content.Enzymatic propertiesSource: MicroorganismEnzymology Committee Number: EC 1.1.3.2Molecular weight: 42 kDa (SDS-PAGE)Isoelectric point: pH 4.6Km value: 7.5 × 10-4 M (L-Lactate)Inhibitors: Hg²⁺, Pb²⁺ Optimum pH: 6.0-7.0 Figure 1 Optimum temperature: 50℃ Figure 2pH stability: 6.0-8.5 (25℃,16h) Figure 3 Thermal stability: Stable below 50℃ (pH7.0, 30min) Figure 4Stability: -25 ~ -15℃ standing store for 12 monthsMore than 90% activity Figure 5 Enzyme activity definitionUnit enzyme activity is defined as the amount of enzyme required to catalyze the production of 1µmol H2O2 per minute under the following conditions.Assay method for activity1. PrincipleThe amount of Quinoneimine dye produced by the reaction can be measured by spectrophotometer at 555 nm.2. Definition of enzyme activityUnit enzyme activity is defined as the amount of enzyme required to catalyze the production of 1 µmol H2O2 per minute under the following conditions.3. Reagent preparationReagent I: 0.2 MpH 6.5 potassium phosphate buffer.Reagent II: 1kU/mL peroxidase (POD) solution.Reagent III: 50 mM4-AA solution.Reagent IV: 0.5 MDL- lactic acid solution, pH6.5.Reagent V: 50 mMTOOS solution.Enzyme diluent: 10 mMpH7.0 potassium phosphate buffer containing 10 µM FAD.Sample: Dilute the enzyme with enzyme diluent to 0.05-0.2U/mL.Prepare the reaction mixture as follows:Reagent I is 10 mlReagent II 0.25 mLReagent III 1.5 mLReagent IV is 5 mLReagent V 1.5 mLDouble steam water to 50 ml4. Operation procedure4.1 Add 1mL reaction mixture into 1mL colorimetric dish.4.2 Preheat the reaction mixture at 37 °C for 5min.4.3 Add 20µL of enzyme liquid to be measured and mix well.4.4 The reaction is measured at 37 °C at 555 nm and the absorbance change (∆As) within 1min is recorded.* Replace enzyme liquid with enzyme diluent, other steps are the same, the absorbance of the resulting solution is blank absorbance (∆Ab)∆A=∆As-∆Ab5. Vitality computing1.020: total volume of reaction liquid (mL);0.020: enzyme liquid volume (mL);1: optical path length (cm);1/2:1 mole of hydrogen peroxide to generate 1/2 mole of quinone imide dye;df: dilution ratio;C: Enzyme concentration (mg/mL);39.2: Under standard reaction conditions, the millimolar absorption coefficient of the color group at 555 nm (cm2/µmol)... Read More | Inquire | Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) TFA is a minor component of CNS myelin. Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat TFA has encephalitogenic activity and induces T cell proliferative. Myelin Oligodendrocyte Glycoprotein Peptide Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat (MOG (35-55)) TFA is a minor component of CNS myelin. Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat TFA has encephalitogenic activity and induces T cell proliferative. Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat TFA induces Th1 cytokine response as well as relatively high levels of IgG antibodies. Myelin Oligodendrocyte Glycoprotein Peptide (35-55), mouse, rat TFA produces a relapsing-remitting neurological disease with extensive plaque-like demyelination... Read More | Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description:Cyclophilin B (SCYLP, CyPB, and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description:Cyclophilin B (SCYLP, CyPB, and peptidyl-prolyl cis-trans isomerase B) is a 24 kDa glycoprotein member of the B subfamily of the cyclophilin-type PPIase family of molecules. It is both secreted and retained in the ER. When secreted, it mediates chemotaxis and T cell adhesion to fibronectin. This is likely due to its prolyl cis/trans isomerase activity. Intracellularly, Cyclophilin B appears to serve as a molecular chaperone for molecules destined for secretion. It does so via stabilization and facilitating the activity of additional chaperones. The human CyPB precursor is 216 amino acids (aa) in length. It contains a 25 aa signal sequence plus a 191 aa mature region. There is a partial heparin-binding sequence (aa 27‑34), a PPIase domain (aa 47‑204), and a C-terminal ER retention motif (aa 213‑216). Over aa 34‑216, the human and mouse sequences are 95% aa identical... Read More | Purity>95% SDS-PAGE. Protein Content and Purity (typically = 95%) determined by reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm.Additional sequence informationThis product is for the mature full length protein. The signal peptide is not included.FunctionCytokine with a wide variety Purity>95% SDS-PAGE. Protein Content and Purity (typically = 95%) determined by reducing and Non-reducing SDS-PAGE, UV spectroscopy at 280 nm.Additional sequence informationThis product is for the mature full length protein. The signal peptide is not included.FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.Post-translational:N- and O-glycosylated... Read More |