| Description | Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus fulvidraco, Mugil cephalus, and Neosciurus carolinensis (gray squirrel) origin.This product can be used as a positive control in fluorescence in situ hybridization (FISH) assay of target RNA.This product is a 21nt single-stranded DNA sequence of 18S rRNA and can specifically recognize and hybridize 18S rRNA. It is labeled with the red fluorescent dye Cy3 at the 5' end, with a maximum excitation wavelength of 550nm and a maximum emission wavelength of 570nm.The recommended pre-hybridization and hybridization temperature for this product is 45ºC. Please refer to Figure 1 for the hybridization effect of this product on 18S rRNA in Hela cells.Figure 1. The in situ hybridization of 18S rRNA in Hela cells using Aladdin's 18S rRNA Cy3 FISH Probe and Fluorescence in Situ Hybridization Kit for RNA. A. Negative control assay with the Cy3-18S rRNA sense probe; B. Negative control cells stained with DAPI; C. The merge image of A and B; D. Assay of specimen with the 18S rRNA Cy3 FISH Probe ; E. Specimen stained with DAPI; F. The merge image of D and E. Scale Bars are 100µm. This figure is for reference only, which may vary due to different experimental conditions.The concentration of this product is 1mg/ml. For fluorescence in situ hybridization, the recommended working concentration of 18S rRNA Cy3 FISH Probe is 0.5-1µg/ml.Precautions：This product should be protected from light to minimize the quenching of fluorescence.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：Please refer to the instructions of ’s Fluorescence in Situ Hybridization Kit for RNA... Read More | Cardiolipin is a unique phospholipid present in the inner mitochondrial membrane, which makes up to 20% of total lipids. It is a non-bilayer anionic phospholipid, which has four acyl chains and small headgroupHeart CA has been used as a standard stock solution for its quantitative analysis using Cardiolipin is a unique phospholipid present in the inner mitochondrial membrane, which makes up to 20% of total lipids. It is a non-bilayer anionic phospholipid, which has four acyl chains and small headgroupHeart CA has been used as a standard stock solution for its quantitative analysis using liquid chromatography?mass spectrometry (LC-MS)/MS. It has also been used for liposome preparation... Read More | Laccase is an enzyme, produced by ericoid mycorrhiza and ectomycorrhiza fungi. It belongs to the group of polyphenol oxidases. Laccase is also present in plants and bacteria.Laccase from Trametes versicolor has been used: to assess the use of four laccase-producing strains in waste water treatment Laccase is an enzyme, produced by ericoid mycorrhiza and ectomycorrhiza fungi. It belongs to the group of polyphenol oxidases. Laccase is also present in plants and bacteria.Laccase from Trametes versicolor has been used: to assess the use of four laccase-producing strains in waste water treatment in laccase assay in screening the lignolsSome of the enzymatic actions of laccase are associated with sporulation, detoxification, morphogenesis, melanin polymerization and it offers protection to spore coat. Laccase can catalyse a number of substrates including medicinal drugs and halogenated pesticides. It utilizes oxygen for its catalysis. For these reasons, it might be useful in the biological degradation of micropollutants in wastewater treatment. Laccase catalyzes the oxidation of phenol containing compounds, including lignin, through the reduction of oxygen to water. The presence of mediators will allow the oxidation of non-phenlic compounds as well. The primary function of laccase is to degrade lignin in fungi... Read More | Inquire | Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard Purity: >95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： CD4, also known as L3T4, T4, and W3/25, is an approximately 55 kDa type I transmembrane glycoprotein that is expressed predominantly on thymocytes and a subset of mature T lymphocytes. It is a standard phenotype marker for the identification of T cell populations. Mature human CD4 consists of a 371 amino acid (aa) extracellular region containing four immunoglobulin-like domains, a 22 aa transmembrane segment, and a 40 aa cytoplasmic domain. Within the ECD, human CD4 shares approximately 52% aa sequence identity with mouse and rat CD4. CD4 is expressed along with CD8 on double positive T cells during their development in the thymus. Either CD4 or CD8 expression is then lost, giving rise to single positive (SP) CD4+ or CD8+ mature T cells. CD4+ SP cells, also known as T helper cells, further differentiate into multiple subsets of CD4+ cells including Th1, Th2, Th17, Tfh, and Treg cells which regulate humoral and cellular immunity. CD4 is reexpressed on circulating CD8+ T cells upon activation and contributes to their cytotoxic effector activity. In human, CD4 is additionally expressed on macrophages, neutrophils, monocytes, NK cells, and neurons and glial cells in the brain. Similar CD4 distribution between species cannot be assumed as demonstrated by its presence on macrophages in human and rat but not in mouse. CD4 binds directly to MHC class II molecules on antigen presenting cells. This interaction contributes to the formation of the immunological synapse which is focused around the TCR-MHC class II-antigenic peptide interaction. Palmitoylation of two cysteine residues in the cytoplasmic tail of CD4 promotes the localization of CD4 in lipid rafts and its ability to augment TCR signaling via activation of the tyrosine kinase Lck. CD4 also functions as a chemotactic receptor for IL-16 and, in human, as a co-receptor for the gp120 surface glycoprotein of HIV-1... Read More |