| Description | Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus fulvidraco, Mugil cephalus, and Neosciurus carolinensis (gray squirrel) origin.This product can be used as a positive control in fluorescence in situ hybridization (FISH) assay of target RNA.This product is a 21nt single-stranded DNA sequence of 18S rRNA and can specifically recognize and hybridize 18S rRNA. It is labeled with the red fluorescent dye Cy3 at the 5' end, with a maximum excitation wavelength of 550nm and a maximum emission wavelength of 570nm.The recommended pre-hybridization and hybridization temperature for this product is 45ºC. Please refer to Figure 1 for the hybridization effect of this product on 18S rRNA in Hela cells.Figure 1. The in situ hybridization of 18S rRNA in Hela cells using Aladdin's 18S rRNA Cy3 FISH Probe and Fluorescence in Situ Hybridization Kit for RNA. A. Negative control assay with the Cy3-18S rRNA sense probe; B. Negative control cells stained with DAPI; C. The merge image of A and B; D. Assay of specimen with the 18S rRNA Cy3 FISH Probe ; E. Specimen stained with DAPI; F. The merge image of D and E. Scale Bars are 100µm. This figure is for reference only, which may vary due to different experimental conditions.The concentration of this product is 1mg/ml. For fluorescence in situ hybridization, the recommended working concentration of 18S rRNA Cy3 FISH Probe is 0.5-1µg/ml.Precautions：This product should be protected from light to minimize the quenching of fluorescence.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：Please refer to the instructions of ’s Fluorescence in Situ Hybridization Kit for RNA... Read More | Inquire | Source: Microorganism Isoelectric point: 6.5 Michaelis constant: 9.2×10^-3 M (D-Glucose); 8.6×10^-3 M (NAD) Optimum pH: 9.0~9.5 Fig. 1Optimum temperature: 55℃ Fig. 3pH Stability: 6.0-10.0 (25℃, 24hr) Fig. 2Thermal stability: <50℃ (pH 8.0, Source: Microorganism Isoelectric point: 6.5 Michaelis constant: 9.2×10^-3 M (D-Glucose); 8.6×10^-3 M (NAD) Optimum pH: 9.0~9.5 Fig. 1Optimum temperature: 55℃ Fig. 3pH Stability: 6.0-10.0 (25℃, 24hr) Fig. 2Thermal stability: <50℃ (pH 8.0, 30min) Fig. 4Inhibitors: NEM,SDS Effect of various chemicals: Table 1Reaction:... Read More | Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a Product Application：KNK437 has been used: as a heat shock factor 1 (HSF1) inhibitor to study its effects on the inhibition of viability and apoptosis activation in chemoresistant mice cells as an HSF1 inhibitor to study its effects on viability and apoptosis of colorectal cancer cells as a heat shock protein 70 (HSP70) inhibitor to study its effects on glutamine-induced HSP70 and inflammatory mediator release... Read More | Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work Purified pectinase is a multi-component preparation highly effective in depolymerizing plant pectins with varying degrees of esterification. The product contains substantial hemicellulase, cellulase, pectinesterase and xylanase activities which together with pectin lyase and polygalacturonase work synergistically to digest plant cell wall tissues. When used with Worthington purified cellulase, purified pectinase has been found to be highly successful for generating good yields of viable protoplasts in several plant systems, e.g., corn, soybean, red beet, sunflower, tomato and citrus. In general, a concentration range of 0.1% to 0.5% pectinase (with accompanying 0.5% to 1.5% cellulase) used at 24°C to 37°C for periods of 1 to 16 hours will yield good results... Read More |