| Description | Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus fulvidraco, Mugil cephalus, and Neosciurus carolinensis (gray squirrel) origin.This product can be used as a positive control in fluorescence in situ hybridization (FISH) assay of target RNA.This product is a 21nt single-stranded DNA sequence of 18S rRNA and can specifically recognize and hybridize 18S rRNA. It is labeled with the red fluorescent dye Cy3 at the 5' end, with a maximum excitation wavelength of 550nm and a maximum emission wavelength of 570nm.The recommended pre-hybridization and hybridization temperature for this product is 45ºC. Please refer to Figure 1 for the hybridization effect of this product on 18S rRNA in Hela cells.Figure 1. The in situ hybridization of 18S rRNA in Hela cells using Aladdin's 18S rRNA Cy3 FISH Probe and Fluorescence in Situ Hybridization Kit for RNA. A. Negative control assay with the Cy3-18S rRNA sense probe; B. Negative control cells stained with DAPI; C. The merge image of A and B; D. Assay of specimen with the 18S rRNA Cy3 FISH Probe ; E. Specimen stained with DAPI; F. The merge image of D and E. Scale Bars are 100µm. This figure is for reference only, which may vary due to different experimental conditions.The concentration of this product is 1mg/ml. For fluorescence in situ hybridization, the recommended working concentration of 18S rRNA Cy3 FISH Probe is 0.5-1µg/ml.Precautions：This product should be protected from light to minimize the quenching of fluorescence.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use：Please refer to the instructions of ’s Fluorescence in Situ Hybridization Kit for RNA... Read More | Es Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5 '→ 3' DNA polymerase activity, 5 '→ 3' exonuclease activity, and 3 '→ 5' exonuclease activity. Compared with Taq DNA Polymerase, Es Taq DNA Polymerase has excellent performance of high Es Taq DNA Polymerase is an optimized mixed enzyme of Taq and Pfu DNA Polymerase, with 5 '→ 3' DNA polymerase activity, 5 '→ 3' exonuclease activity, and 3 '→ 5' exonuclease activity. Compared with Taq DNA Polymerase, Es Taq DNA Polymerase has excellent performance of high amplification efficiency and low mismatch rate, and can efficiently amplify DNA fragments. Most of the PCR products amplified with this product contain an "A" base at the 3 'end, which can be directly used for T/A cloning. This product is suitable for conventional PCR reactions and gene cloning reactions that require high fidelity. E665597Component500 UStorageE665597AEs Taq DNA Polymerase, 5 U/µL 100 µL -20℃. Avoid freeze/thaw cycle.E665597B10×PCR Buffer 1.8 mL -20℃. Avoid freeze/thaw cycle.Activity definition:Using activated salmon sperm DNA as a template/primer, the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into acidic insoluble substances is defined as 1 active unit (U) at 74 ℃ for 30 minutes.Quality control:After multiple column purifications, SDS-PAGE detected a purity of over 99%; No exogenous nuclease activity detected; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes in the human genome; Store at room temperature for one month without significant changes in activity.1. PCR reaction system Reagent 50 µlReaction system Final concentration 10×PCR Buffer 5 µL 1× dNTP Mix，10 mM each 1 µL 200 µM each Forward Primer，10 µM 2 µL 0.4 µM Reverse Primer，10 µM 2 µl 0.4 µM Template DNA <0.5 µg <0.5 µg/50 µl Es Taq DNA Polymerase，5 U/µl 0.25-0.5 µl 1.25-2.5U/50 µl ddH2O up to 50 µL /Attention: The primer concentration should be between 0.1 and 1.0 as the final concentration µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system. 2. PCR reaction conditions Step Temperature Time / Pre denaturation 94℃ 2 min / Denaturation 94℃ 30 s 25-35 cycles Anneal 55-65℃ 30 s 25-35 cycles Extend 72℃ 30 s 25-35 cycles Finally extended 72℃ 2 min / Attention:1) In general experiments, if the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of Es Taq DNA Polymerase in this product is 2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Laccase is an enzyme, produced by ericoid mycorrhiza and ectomycorrhiza fungi. It belongs to the group of polyphenol oxidases. Laccase is also present in plants and bacteria.Laccase from Trametes versicolor has been used: to assess the use of four laccase-producing strains in waste water treatment Laccase is an enzyme, produced by ericoid mycorrhiza and ectomycorrhiza fungi. It belongs to the group of polyphenol oxidases. Laccase is also present in plants and bacteria.Laccase from Trametes versicolor has been used: to assess the use of four laccase-producing strains in waste water treatment in laccase assay in screening the lignolsSome of the enzymatic actions of laccase are associated with sporulation, detoxification, morphogenesis, melanin polymerization and it offers protection to spore coat. Laccase can catalyse a number of substrates including medicinal drugs and halogenated pesticides. It utilizes oxygen for its catalysis. For these reasons, it might be useful in the biological degradation of micropollutants in wastewater treatment. Laccase catalyzes the oxidation of phenol containing compounds, including lignin, through the reduction of oxygen to water. The presence of mediators will allow the oxidation of non-phenlic compounds as well. The primary function of laccase is to degrade lignin in fungi... Read More | Inquire | Inquire |