| Description | Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus Aladdin's 18S rRNA Cy3 FISH Probe can be used in conjunction with the Fluorescence in Situ Hybridization Kit for RNA to analyze the spatial and temporal distribution of 18S rRNA in cells, fixed tissues, and paraffin or frozen sections of human, mouse, rat, Prionailurus viverrinus, Tachysurus fulvidraco, Mugil cephalus, and Neosciurus carolinensis (gray squirrel) origin.This product can be used as a positive control in fluorescence in situ hybridization (FISH) assay of target RNA.This product is a 21nt single-stranded DNA sequence of 18S rRNA and can specifically recognize and hybridize 18S rRNA. It is labeled with the red fluorescent dye Cy3 at the 5' end, with a maximum excitation wavelength of 550nm and a maximum emission wavelength of 570nm.The recommended pre-hybridization and hybridization temperature for this product is 45ºC. Please refer to Figure 1 for the hybridization effect of this product on 18S rRNA in Hela cells.Figure 1. The in situ hybridization of 18S rRNA in Hela cells using Aladdin's 18S rRNA Cy3 FISH Probe and Fluorescence in Situ Hybridization Kit for RNA. A. Negative control assay with the Cy3-18S rRNA sense probe; B. Negative control cells stained with DAPI; C. The merge image of A and B; D. Assay of specimen with the 18S rRNA Cy3 FISH Probe ; E. Specimen stained with DAPI; F. The merge image of D and E. Scale Bars are 100µm. This figure is for reference only, which may vary due to different experimental conditions.The concentration of this product is 1mg/ml. For fluorescence in situ hybridization, the recommended working concentration of 18S rRNA Cy3 FISH Probe is 0.5-1µg/ml.Precautions:This product should be protected from light to minimize the quenching of fluorescence.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.Instructions for Use:Please refer to the instructions of ’s Fluorescence in Situ Hybridization Kit for RNA... Read More | Inquire | Inquire | Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description:Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.Epitope tagging offers an easy and universalPurity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description:Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product... Read More | Purity> 95 % by SDS-PAGE and HPLC analyses.FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix Purity> 95 % by SDS-PAGE and HPLC analyses.FunctionPromotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CYR61-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.Banckground:Cyr61, also known as CCN1, is a 40-45 kDa matricellular glycoprotein that plays an important role in cellular adhesion and migration (1). Cyr61 consists of an IGFBP domain, a VWF type C domain, a TSP type I domain, and a cysteine knot domain (2). Mature human Cyr61 shares 93% amino acid sequence identity with mouse and rat Cyr61. It is widely expressed during development and in adult tissues (2, 3). Cyr61 associates with the extracellular matrix (ECM) and with many cell surface molecules including Integrins alpha V beta 3, alpha V beta 5, alpha M beta 2, and alpha 6 beta 1, Syndecan-4, and heparan sulfate proteoglycans (1, 3). Cyr61 mediates the adhesion and migration of multiple cell types and also promotes vascular endothelial cell tubule formation (4-6). Plasmin cleavage of ECM-bound Cyr61 releases a 28 kDa N-terminal fragment which retains the ability to promote endothelial cell migration (7). Cyr61 exhibits both tumorigenic and tumor suppressor properties. It is up-regulated and promotes tumorigenesis, angiogenesis, and metastasis in breast, renal, gastric, squamous cell, and colorectal carcinomas as well as in glioma (8-12). In contrast, whendown-regulated, it suppresses tumor growth in endometrial, hepatic, and non-small cell lung cancers (8, 13, 14). Cyr61 is also up-regulated in injured skin and bone where it induces the expression of growth factors, cytokines, proteases, and integrins involved in wound repair (15, 16)... Read More |