| Description | If the serum will not be used up within a short period of time, dispense after thawing and store aliquot under appropriate conditions for long-term storage. The volume of serum will increase by 10% after freezing, so when dispensing serum into tubes, leave some space in the tube to avoid tube If the serum will not be used up within a short period of time, dispense after thawing and store aliquot under appropriate conditions for long-term storage. The volume of serum will increase by 10% after freezing, so when dispensing serum into tubes, leave some space in the tube to avoid tube cracking during the freezing.After the serum is thawed completely, the serum can be heated at 56℃ for 30 minutes to inactivate the complement in serum. Unless necessary, heat-inactivation of the serum is not recommended for general use because heat treatment will result in precipitation of serum and decreasing of serum quality. Complement is involved in reactions such as cytotoxicity, contraction of smooth muscle cells, release of histamine from mast cells and platelets, enhancement of phagocytosis, and promotion of chemical chemotaxis and activation of lymphocytes and macrophages.The serum should be thawed slowly and gradually. Thaw the serum stored at -15~-40℃ in 4℃ refrigerator for approximately one day, and make aliquot after it is thawed thoroughly. During the thawing process, shake gently every 2 hours (avoid the formation of air bubbles) to homogenize the component and reduce the occurrence of precipitation. Do not thaw the serum stored at -20℃ or lower temperatures directly in a 37℃ water bath to avoid protein agglomeration and precipitation in serum due to large temperature changes.The floc-like precipitates in serum are mainly denatured fibrin and lipoproteins in serum after long-term storage at 4℃. These flocs do not affect the quality of the serum and can be left untreated. If necessary, the flocs can be removed by centrifugation at 400×g for 5 minutes. However, it is not advisable to remove by filtration as the flocs may block the filter membrane. The precipitates in the serum after heat inactivation will increase significantly. Some of these deposits look like "black dots" under the microscope and are often misinterpreted as microbial contamination because the Brownian motion of these "dots" is magnified under the microscope and appear to be swimming. Usually, these small black spots do not affect cell growth, but if microbial contamination of the serum is suspected, it should be immediately replaced with new serum. Users can dilute the serum with culture medium to 10% concentration and incubate for 1-3 days, and observe whether the small black dots increase sharply, or spread an appropriate amount of serum on LB plate to determine whether there is microbial contamination. Do not leave the serum at 37℃ for too long, as the serum will gradually become cloudy and lose some active ingredients, thus affecting the quality of the serum.This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation... Read More | Inquire | 2x Taq MasterMix is a premixed system composed of Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers, and enhancers. The pre prepared PCR mixture makes the operation simpler and faster, and can minimize human error and contamination to the greatest extent possible. The original MasterMix formula 2x Taq MasterMix is a premixed system composed of Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers, and enhancers. The pre prepared PCR mixture makes the operation simpler and faster, and can minimize human error and contamination to the greatest extent possible. The original MasterMix formula results in high yield, strong repeatability, and good stability of amplified products. This product does not contain dyes. After the PCR program is completed, an appropriate amount of sample buffer can be added as needed for electrophoresis operation. The amplified PCR product has an "A" base attached to the 3 'end, making it suitable for direct use in T/A cloning. Mainly suitable for PCR amplification of DNA, DNA sequencing and other experiments.Quality control: T665627Component5mlStorageT665627A2×Taq MasterMix5×1ml-20℃. Avoid freeze/thaw cycle.T665627BddH₂O5×1ml-20℃. Avoid freeze/thaw cycle.Notes: 2×Taq MasterMix contains Taq DNA Polymerase, 3mM MgCl2 and 400µM each dNTP After testing, there was no exogenous nuclease activity; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes from multiple genomes.Usage:The following is an example of a PCR reaction system and reaction conditions for amplifying a 1 kb fragment using human genomic DNA as a template. In practical operation, corresponding improvements and optimizations should be made based on the template, primer structure, and target fragment size.1. PCR reaction systemReagent50 µlReaction systemFinal concentration2×Taq MasterMix25 µl1×Forward Primer，10 µM2 µl0.4 µMReverse Primer，10 µM2 µl0.4 µMTemplate DNA<0.5 µg<0.5 µg/50 µlddH2Oup to 50 µl/Attention: The primer concentration should be between 0.1 and 1.0 as the final concentration µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system.2. PCR reaction conditionsStepTemperatureTime/Pre denaturation95℃2 min/Denaturation94℃30 s25-35 cyclesAnneal55-65℃30 s25-35 cyclesExtend72℃30 s25-35 cyclesFinally extended72℃2 min/Attention:1) In general experiments, if the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of Taq DNA Polymerase in this product is 2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Heme Oxygenase-1-IN-1 (Compound 2) is a heme oxygenase 1 ( HO-1 ) inhibitor with an IC 50 of 0.25 µMIC50& Target:IC 50 : 0.25 µM (HO-1) | Purity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.Epitope tagging offers an easy and universalPurity: >90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Involved in the high-affinity maltose membrane transport system MalEFGK. Initial receptor for the active transport of and chemotaxis toward maltooligosaccharides.Epitope tagging offers an easy and universal strategy for the identification and purification of proteins derived by recombinant DNA technology. The insertion of a Maltose Binding Protein (MBP) tag creates a stable fusion product that does not interfere with the bioactivity of the protein or with the biodistribution of the MBP tagged product... Read More |