| Description | Collagenase is derived from Clostridium histolyticum and is a type of protease. It can specifically recognize the Pro-X-Gly-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) in this sequence, and this sequence appears very frequently in collagen. Collagenase is derived from Clostridium histolyticum and is a type of protease. It can specifically recognize the Pro-X-Gly-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) in this sequence, and this sequence appears very frequently in collagen. Collagenase is the only protease that can degrade natural collagen fibers with a triple-helix structure, which are widely present in connective tissues. This product is Collagenase Type I with an enzyme activity of ≥125 U/mg solid, and it can be used for the dissociation of tissues and cells such as epithelium, liver, lung, fat, and adrenal gland.Usage Instructions(I) Preparation of Collagenase Stock SolutionAdd 1 ml of HBSS (Hank's Balanced Salt Solution) containing Ca²⁺ and Mg²⁺ to each tube of 100 mg collagenase, gently vortex to dissolve it fully, and prepare a stock solution with a concentration of 100 mg/ml (i.e., 100×). Filter and sterilize using a 0.22 µm filter membrane with low protein binding property, aliquot into small portions, and store at -20℃ in the dark.Thaw on ice before use, and avoid repeated freezing and thawing. Common concentrations: the common concentration for tissue and cell dispersion is 0.5~2.5 mg/ml, and the common concentration for cartilage digestion is 1~2 mg/ml; the optimal working concentration should be determined according to specific experimental conditions or by referring to relevant literature.(II) Tissue SeparationCut the tissue into tissue blocks of 3~4 mm in size using a sterile scalpel or scissors;Wash the tissue blocks several times with HBSS containing Ca²⁺ and Mg²⁺;Add a sufficient amount of HBSS containing Ca²⁺ and Mg²⁺ to submerge the tissue blocks, and add collagenase to the required working concentration;Incubate at 37℃ for 4~18 hours; using a horizontal shaker during digestion and supplementing digestion with 3 mM CaCl₂ can improve digestion efficiency;Sieve the dispersed cells using a stainless steel or nylon mesh sieve, collect and set aside; for tissues that are not completely dissociated, add an appropriate amount of fresh collagenase working solution and continue incubation at 37℃;Wash the collected cells several times with HBSS without collagenase;Resuspend the above cells with cell culture medium, and calculate the viable cell density using an automatic cell counter or other methods;Inoculate the cells on a cell culture dish using a suitable cell culture medium.(III) Organ PerfusionAdd collagenase to HBSS containing Ca²⁺ and Mg²⁺ preheated to 37℃; additionally adding 3 mM CaCl₂ helps improve separation efficiency;Perfuse the corresponding organ with the collagenase working solution at an optimized rate;Pass the perfusion solution recovered in the above process through a stainless steel or nylon mesh sieve to separate the dissociated cells or small tissue fragments from larger clumps; for tissues that are not fully dissociated, further incubate at 37℃ with fresh collagenase working solution;Wash the collected cells several times with HBSS without collagenase;Resuspend the above cells with cell culture medium, and calculate the viable cell density using an automatic cell counter or other methods;Inoculate the cells on a cell culture dish using a suitable cell culture medium... Read More | description：Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.Bovine pancreas is a rich source of RNase A which is often found in many description：Bovine pancreatic deoxyribonuclease I produced recombinantly in yeast, Pichia pastoris, to decrease levels of contaminating RNase and eliminate potential pathogens associated with animal based materials.Bovine pancreas is a rich source of RNase A which is often found in many commercial DNase preparations. Producing DNase I by recombinant means in an organism with much lower levels of endogenous RNase greatly facilitates purification of an enzyme with undetectable levels of RNase. The processes involved in the production and isolation of recombinant DNase I are completely devoid of animal based components which eliminates the possibility of introducing animal derived pathogens into bioprocessing procedures.Animal Free/AF. Recombinant Bovine pancreatic deoxyribonuclease 1 produced in Pichia pastoris. Chromatographically purified. Free of animal derived components, RNases & proteases. A liquid preparation in 5mM Calcium Acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol. Supplied with 10x reaction buffer.Storage Buffer : 5mM calcium acetate, 4mg/ml glycine, pH 5.0 and 50% glycerol.DNase I Reaction Buffer (10X): 500mM Tris-HCl, 10mM MgSO4, 1mM CaCl2, pH 7.8, provided.application：Recombinant DNase I is suitable for such applications as:• Removing genomic DNA from RNA preparations prior to RT-PCR• Degradation of DNA templates after transcription reactions• Removing unwanted DNA from samples prior to Northern blotting• Removing DNA during biopharma and bioprocessing procedures... Read More | Inquire | Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity Purity:>90%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Receptors that recognize the Fc portion of IgG are divided into three groups designated Fc gamma RI, RII, and RIII, also known respectively as CD64, CD32, and CD16. Fc gamma RI binds IgG with high affinity and functions during early immune responses. Fc gamma RII and RIII are low affinity receptors that recognize IgG as aggregates surrounding multivalent antigens during late immune responses.High affinity immunoglobulin gamma Fc receptor I is also known as FCGR1A, FCG1, FCGR1, CD64 and IGFR1, is a type of integral membrane glycoprotein that binds monomeric IgG-type antibodies with high affinity, which belongs to the immunoglobulin superfamily or FCGR1 family. FCGR1A / CD64 contains 3 Ig-like C2-type (immunoglobulin-like) domains. CD64 is constitutively found on only macrophages and monocytes, but treatment of polymorphonuclear leukocytes with cytokines like IFNγ and G-CSF can induce CD64 expression on these cells... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within the receptor tyrosine kinases (RTK) superfamily. Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue StainingDescription:ROR1 (Receptor tyrosine kinase-like orphan receptor 1), also known as neurotrophic tyrosine kinase receptor-related 1 (NTRKR1), is a member of the ROR family within the receptor tyrosine kinases (RTK) superfamily. Two ROR family members (ROR1 and ROR2) have been identified and are characterized by their intracellular tyrosine kinase domains, which are highly related to those of the Trk-family receptor tyrosine kinases, and by their extracellular Frizzled-like cysteine-rich domains and kringle domains, common to receptors of the Wnt family members. Human ROR1 is a type I transmembrane protein with 937 amino acids in length. It contains a 29 amino acid signal sequence, a 377 amino acid extracellular domain (ECD), a 21 amino acid transmembrane segment, and a 510 amino acid cytoplasmic region. Human ROR1 shares 97% and 58% amino acid sequence identity with mouse ROR1 and human ROR2, respectively. ROR1 has been shown to play crucial roles in developmental morphogenesis by acting as receptors or co-receptors to mediate Wnt5a-induced signaling. The bioactivity of ROR1 is measured by its ability to bind biotinylated recombinant mouse Wnt-5a in a functional ELISA... Read More |