| Description | Collagenase is derived from Clostridium histolyticum and is a type of protease. It can specifically recognize the Pro-X-Gly-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) in this sequence, and this sequence appears very frequently in collagen. Collagenase is derived from Clostridium histolyticum and is a type of protease. It can specifically recognize the Pro-X-Gly-Pro sequence and cleave the peptide bond between the neutral amino acid (X) and glycine (Gly) in this sequence, and this sequence appears very frequently in collagen. Collagenase is the only protease that can degrade natural collagen fibers with a triple-helix structure, which are widely present in connective tissues. This product is Collagenase Type I with an enzyme activity of ≥125 U/mg solid, and it can be used for the dissociation of tissues and cells such as epithelium, liver, lung, fat, and adrenal gland.Usage Instructions(I) Preparation of Collagenase Stock SolutionAdd 1 ml of HBSS (Hank's Balanced Salt Solution) containing Ca²⁺ and Mg²⁺ to each tube of 100 mg collagenase, gently vortex to dissolve it fully, and prepare a stock solution with a concentration of 100 mg/ml (i.e., 100×). Filter and sterilize using a 0.22 µm filter membrane with low protein binding property, aliquot into small portions, and store at -20℃ in the dark.Thaw on ice before use, and avoid repeated freezing and thawing. Common concentrations: the common concentration for tissue and cell dispersion is 0.5~2.5 mg/ml, and the common concentration for cartilage digestion is 1~2 mg/ml; the optimal working concentration should be determined according to specific experimental conditions or by referring to relevant literature.(II) Tissue SeparationCut the tissue into tissue blocks of 3~4 mm in size using a sterile scalpel or scissors;Wash the tissue blocks several times with HBSS containing Ca²⁺ and Mg²⁺;Add a sufficient amount of HBSS containing Ca²⁺ and Mg²⁺ to submerge the tissue blocks, and add collagenase to the required working concentration;Incubate at 37℃ for 4~18 hours; using a horizontal shaker during digestion and supplementing digestion with 3 mM CaCl₂ can improve digestion efficiency;Sieve the dispersed cells using a stainless steel or nylon mesh sieve, collect and set aside; for tissues that are not completely dissociated, add an appropriate amount of fresh collagenase working solution and continue incubation at 37℃;Wash the collected cells several times with HBSS without collagenase;Resuspend the above cells with cell culture medium, and calculate the viable cell density using an automatic cell counter or other methods;Inoculate the cells on a cell culture dish using a suitable cell culture medium.(III) Organ PerfusionAdd collagenase to HBSS containing Ca²⁺ and Mg²⁺ preheated to 37℃; additionally adding 3 mM CaCl₂ helps improve separation efficiency;Perfuse the corresponding organ with the collagenase working solution at an optimized rate;Pass the perfusion solution recovered in the above process through a stainless steel or nylon mesh sieve to separate the dissociated cells or small tissue fragments from larger clumps; for tissues that are not fully dissociated, further incubate at 37℃ with fresh collagenase working solution;Wash the collected cells several times with HBSS without collagenase;Resuspend the above cells with cell culture medium, and calculate the viable cell density using an automatic cell counter or other methods;Inoculate the cells on a cell culture dish using a suitable cell culture medium... Read More | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining. Description： This protein is a cell adhesion molecule involved in neuron-neuron adhesion, neurite fasciculation, outgrowth of neurites, etc | Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial Purity:>95%, by SDS-PAGE visualized with Coomassie® Blue Staining.Description：Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL-... Read More | Product IntroductionKGF keratinocyte growth factor (KGF), a cytokine identified by Rubin et al (1989) from the culture supernatant of embryonic lung fibroblasts, is an FGF family member, namely FGF-7.KGF is secreted by stromal cells and its receptor is distributed in epithelial cells, where it is a Product IntroductionKGF keratinocyte growth factor (KGF), a cytokine identified by Rubin et al (1989) from the culture supernatant of embryonic lung fibroblasts, is an FGF family member, namely FGF-7.KGF is secreted by stromal cells and its receptor is distributed in epithelial cells, where it is a potent epithelial cell specific growth factor, and its mitogenic activity is mainly expressed in keratinocytes, which can specifically promote epithelial cell proliferation, migration and differentiation, and is closely related to many aspects, such as organ development, wound repair, tumorigenesis and immune reconstitution.Osrhkgf was created using genetic recombination, expressed from rice endosperm cells and through a protein purification process.Specification parametersSource Oryza sativaAppearance white lyophilized powderActivity ≥1.0×105IU/mgpH 6.5-7.5Molecular weight 19.0 kDEndotoxin ≦0.1EU/ugCAS No 148348-15-6Matters needing attentionReconstitution: it is recommended to lyophilize the powder of osrhkgf to 100-200 UG/ml with sterile water to make further dilutions with other solvents.The dissolved osrhkgf could be stored for 2-7 days at 4 ◦ C and used up as soon as possible.To not use for short periods, store at - 20 ℃.Use as soon as possible after opening to avoid contamination.Limitations of useIt is suitable for research, laboratory and production use only and cannot be used directly in humans... 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