| Description | Gak Rat Pre-designed siRNA Set A contains three designed siRNAs for Gak gene (Rat), as well as a negative control, a positive control, and a FAM-labeled negative control. Components Gak siRNA-1: 5 nmol (HPLC) Gak siRNA-2: 5 nmol (HPLC) Gak siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC)Gak Rat Pre-designed siRNA Set A contains three designed siRNAs for Gak gene (Rat), as well as a negative control, a positive control, and a FAM-labeled negative control. Components Gak siRNA-1: 5 nmol (HPLC) Gak siRNA-2: 5 nmol (HPLC) Gak siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)... Read More | Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations... Read More | Extinction CoeffA280 nm = 1.0 at 1.0 mg/mlGeneral DescriptionProduct is whole polyclonal antiserum from goats immunized with highly purified mouse complement protein. This product is not a purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & Extinction CoeffA280 nm = 1.0 at 1.0 mg/mlGeneral DescriptionProduct is whole polyclonal antiserum from goats immunized with highly purified mouse complement protein. This product is not a purified IgG fraction. Goats are maintained in FDA certified facilities.Physical Characteristics & StructureAntibodies present in the antisera are primarily IgG.ApplicationsImmunodiffusion: Effective against NMS and plasma at 1/32 dilution Suggested starting dilutions:Western Blot: 1/500 to 1/1000. Most effective against non-reduced antigen.ELISA: 1/500 to 1/2000... Read More | Inquire | Keratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelialKeratinocyte growth factor (KGF) is a cytokine found by Rubin et al. (1989) from the culture supernatant of embryonic lung fibroblasts, which is a member of the FGF family, namely FGF-7. KGF is an effective epithelial-specific growth factor secreted by mesenchymal cells and distributed in epithelial cells. Its mitotic activity is mainly manifested in keratinocytes, which can specifically promote the proliferation, migration and differentiation of epithelial cells. It is closely related to organ development, wound repair, tumor genesis and immune reconstruction.Activity definition: The ED50 value is less than 1.0 ng/ml, that is, the corresponding activity unit is greater than or equal to 1 x 10*6 units/mg, as determined by the proliferation method of cultured MCF-7 cells... Read More |