| Description | CYP7B1 Human Pre-designed siRNA Set A contains three designed siRNAs for CYP7B1 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components CYP7B1 siRNA-1: 5 nmol (HPLC) CYP7B1 siRNA-2: 5 nmol (HPLC) CYP7B1 siRNA-3: 5 nmol (HPLC) siRNA Negative CYP7B1 Human Pre-designed siRNA Set A contains three designed siRNAs for CYP7B1 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components CYP7B1 siRNA-1: 5 nmol (HPLC) CYP7B1 siRNA-2: 5 nmol (HPLC) CYP7B1 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)... Read More | Product DescriptionEndo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, Product DescriptionEndo F2 cleaves N-linked (asparagine-linked) biantennary oligosaccharides from glycoproteins. It also will cleave high mannose glycans but at a 40x reduced rate. It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact.Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However, for optimal results, denaturation of the glycoprotein is recommended.Contents60 µl aliquot of enzyme (0.3 U) in 10 mM sodium acetate 25mM NaCl, pH 4.5Included with 20 µL and 60 µL pack sizes:5x Reaction Buffer – 250 mM sodium acetate, pH 4.5Molecular weight 32,000 daltonsSpecific Activity Defined as the amount of enzyme required to catalyze the release of N-linked oligosaccharides from 1 micromole of denatured porcine fibrinogen in 1 minute at 37°C, pH 5.5. Cleavage is monitored by SDS-PAGE (cleaved fibrinogen migrates faster).Formulation The enzyme is provided as a sterile-filtered solution in 10 mM sodium acetate, 25mM NaCl, pH 4.5Specificity Endo F2 cleaves Asparagine-linked biantennary and high mannose glycans (at a 40X reduced rate). It cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine. In contrast, PNGase F removes the oligosaccharide intact. Endoglycosidase F2 is less sensitive to protein conformation than PNGase F and is therefore more suitable for deglycosylation of native proteins. However for optimal results, denaturation of the glycoprotein is recommended.Quality & Purity Endo F2 is tested for contaminating protease as follows: 10 µg of denatured BSA is incubated at 37°C for 24 hours with 2 µl of enzyme. SDS-PAGE analysis of the treated BSA shows no evidence of degradation. The production host strain has been extensively tested and does not produce any detectable glycosidases.Stability Several days exposure to ambient temperatures will not reduce activity. Stable at least 12 months when stored properly.Directions for use 1. Add up to 200 µg of glycoprotein to an Eppendorf tube. Adjust to 38 µl final volume with de-ionized water. 2. Add 10 µl 5x Reaction Buffer 4.5 3. Add 2.0 µl of Endo F2 to the reaction. Incubate 1 hour at 37°C. Monitor cleavage by SDS-PAGEThe production host strain has been extensively tested and does not produce any detectable glycosidases... Read More | Lipase PS is generally used in the enantioselective transesterification and hydrolysis. Applications include: 1.Lipase catalyzed transesterification of prochiral pyrimidine acyclonucleoside. 2.Lipase catalyzed hydrolysis of diacetylated pyrimidine acyclonucleosides. 3. Enantiomer selective acylationLipase PS is generally used in the enantioselective transesterification and hydrolysis. Applications include: 1.Lipase catalyzed transesterification of prochiral pyrimidine acyclonucleoside. 2.Lipase catalyzed hydrolysis of diacetylated pyrimidine acyclonucleosides. 3. Enantiomer selective acylation of racemic alcohols in continuous-flow bioreactors... Read More | Purity>98% by SDS-PAGE and HPLC analyses.Additional sequence informationBelongs to the intercrine alpha (chemokine CxC) family.FunctionActs as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in Purity>98% by SDS-PAGE and HPLC analyses.Additional sequence informationBelongs to the intercrine alpha (chemokine CxC) family.FunctionActs as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis (By similarity). Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo.Post-translationalGlycosylated... Read More | Inquire |