| Description | LAMC2 Human Pre-designed siRNA Set A contains three designed siRNAs for LAMC2 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components LAMC2 siRNA-1: 5 nmol (HPLC) LAMC2 siRNA-2: 5 nmol (HPLC) LAMC2 siRNA-3: 5 nmol (HPLC) siRNA Negative Control:LAMC2 Human Pre-designed siRNA Set A contains three designed siRNAs for LAMC2 gene (Human), as well as a negative control, a positive control, and a FAM-labeled negative control. Components LAMC2 siRNA-1: 5 nmol (HPLC) LAMC2 siRNA-2: 5 nmol (HPLC) LAMC2 siRNA-3: 5 nmol (HPLC) siRNA Negative Control: 5 nmol (HPLC) FAM-labeled siRNA Negative Control: 5 nmol (HPLC) GAPDH siRNA Positive Control:5 nmol (HPLC)... Read More | Biochemical Test:SDS-PAGE (purity > 80%); Western blot with patient sample.Calculated Isoelectric Point:pH 6.10 | Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential Crude collagenase preparations contain several isoforms of two different collagenases, a sulfhydryl protease, clostripain, a trypsin-like enzyme, and an aminopeptidase. This combination of collagenolytic and proteolytic activities is effective at breaking down intercellular matrices, the essential part of tissue dissociation. One component of the complex is a hydrolytic enzyme which degrades the helical regions in native collagen preferentially at the Y-Gly bond in the sequence Pro-Y-Gly-Pro, where Y is most frequently a neutral amino acid. This cleavage yields products susceptible to further peptidase digestion. Crude collagenase is inhibited by metal chelating agents such as cysteine, EDTA or o-phenanthroline but not DFP. It is also inhibited by α2-macroglobulin, a large plasma glycoprotein. Ca2+ is required for enzyme activity. Particular enzymatic profiles of each collagenase have been correlated with the tissues from which the cells for study were obtained (or with the uses to which the cells are put) and as a result of the correlations several types of crude collagenases have been established by Aladdin: Types 1, 2, 3, and 4.This collagenase has been tested with cell lines to verify the product is not cytotoxic. Collagenase is typically used to digest the connective components in tissue samples to liberate individual cells. The concentration for cartilage dispersal is 1-2 mg/ml, but literature searches should be performed for species specific and/or tissue specific concentrations... Read More | Lipase PS is generally used in the enantioselective transesterification and hydrolysis. Applications include: 1.Lipase catalyzed transesterification of prochiral pyrimidine acyclonucleoside. 2.Lipase catalyzed hydrolysis of diacetylated pyrimidine acyclonucleosides. 3. Enantiomer selective acylationLipase PS is generally used in the enantioselective transesterification and hydrolysis. Applications include: 1.Lipase catalyzed transesterification of prochiral pyrimidine acyclonucleoside. 2.Lipase catalyzed hydrolysis of diacetylated pyrimidine acyclonucleosides. 3. Enantiomer selective acylation of racemic alcohols in continuous-flow bioreactors... Read More | Format:1-ComponentEnzyme:Horseradish peroxidase |