| Description | The HeLa protein digestion standard is a protein sample derived from HeLa cells and subjected to enzymatic hydrolysis, which is used for mass spectrometry analysis. Its main functions include quality control, method development, quantitative analysis, protein identification, and modification The HeLa protein digestion standard is a protein sample derived from HeLa cells and subjected to enzymatic hydrolysis, which is used for mass spectrometry analysis. Its main functions include quality control, method development, quantitative analysis, protein identification, and modification analysis. It is an indispensable tool in mass spectrometry analysis, capable of improving the accuracy and reliability of the analysis.Storage and Transportation:Storage: The lyophilized powder is stable for 2 years when stored at -20°C or below. The reconstituted and aliquoted product can be stored at -80°C for 1 year. Avoid repeated freezing and thawing!Transportation: It is recommended to transport with ice packs to maintain low temperature.Operating Procedures: 1.Preparation: It is recommended to dissolve the product with 0.1% FA (formic acid) solution. For example: take 1 tube of 20µg HeLa protein digestion standard lyophilized powder, add 40µL of 0.1% FA (formic acid) solution to dissolve it, and vortex to dissolve. The final concentration is 0.5µg/µL, and aliquot for storage.2.Loading: Use an appropriate amount for loading. Generally, 500ng is recommended for a 60-minute gradient, and 1-2µg for a 120-minute gradient. Inject the peptide standard solution into the mass spectrometer for analysis.Precautions and Disclaimer:1.This product is limited to scientific research use by professionals, and shall not be used for clinical diagnosis or treatment, nor for food or drugs.2.For your safety and health, please wear a lab coat and disposable gloves and masks during operation... Read More | Inquire | Purity>95% SDS-PAGE.FunctionThe soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds toPurity>95% SDS-PAGE.FunctionThe soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1.Post-translationalA soluble short 95 kDa form may be released by proteolytic cleavage from the long membrane-anchored form. O-glycosylated with core 1 or possibly core 8 glycans... Read More | Purity≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.FunctionPro-inflammatory cytokine. Involved in the innate immune response to Purity≥95% SDS-PAGE. Recombinant human MIF, fused to His-tag at N-terminus, was cloned into an E. coli expression vector and was purified to apparent homogeneity by using conventional column chromatography techniques.FunctionPro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity... Read More | Purity> 97 % by SDS-PAGE and HPLC analyses.FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation whichPurity> 97 % by SDS-PAGE and HPLC analyses.FunctionReceptor for TNFSF2/TNF-alpha and homotrimeric TNFSF1/lymphotoxin-alpha. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases (aspartate-specific cysteine proteases) mediating apoptosis. Contributes to the induction of non-cytocidal TNF effects including anti-viral state and activation of the acid sphingomyelinase... Read More |