| Description | Carboxypeptidase B, also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.This product is a recombinant carboxypeptidase B lyophilized powder Carboxypeptidase B, also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.This product is a recombinant carboxypeptidase B lyophilized powder obtained by isolation and purification from recombinant Pichia pastoris, followed by freeze - drying. It is a biochemical - grade genetic engineering enzyme, free from exogenous virus contamination, and does not contain enzyme inhibitors such as DFP, PMSF, and TLCK. It has the same activity and specificity as natural carboxypeptidase B.Product AdvantagesAnimal - free origin: No animal viruses, no pathogenic substances, no exogenous factor contamination, and high safety.High purity and high specific activity.Production scale is over 1000L.Compliance: Production equipment and production environment meet relevant regulatory requirements and conform to GMP guidelines.Complete quality documents: Relevant regulatory support documents can be provided according to customer needs.Product CharacteristicsAppearance: White or off - white powderEnzyme specific activity: ≥150 U/mg proteinMolecular weight: 36kD ± 3.6kDPurity: ≥90% (SDS - PAGE)Protein content: ≥30%Optimal pH: 7.0 - 9.0Solubility: Soluble in buffer solutionRecommended Usage MethodsPreparation of proteinDissolve the protein in a 25mM Tris - HCl solution with pH 7.6.Preparation of enzyme solutionWeigh the required enzyme powder and dissolve it in 25mM Tris - HCl (pH 7.6) to make the enzyme solution concentration 1 - 10mg/ml.ReactionRecommended enzyme amount: Target protein (mass ratio) = 1:50 - 1:1000, and the optimal pH is 7.0 - 9.0.Product ApplicationsProduction of various proteins or polypeptides, such as insulin and its analogs, beauty peptides, antimicrobial peptides, food flavor peptides, etc.Quality detection of antibodies.Determination of amino acids at the C - terminus of proteins.Storage stabilityThe lyophilized powder can be stably stored for at least 24 months at -15°C or below. After being dissolved in 25 mM Tris-HCl (pH 7.6) and stored at -15°C or below, there is no loss of activity even after 10 cycles of repeated freezing and thawing. It is recommended that the dissolved product be stored at 2–8°C for no more than 7 days, and at -15°C or below for no more than 30 days.Precautions and DisclaimerIts activity is competitively inhibited by arginine and lysine; metal ion chelators (such as EDTA) also have an inhibitory effect on the enzyme activity.This product is hygroscopic and should be equilibrated at room temperature before being taken and used.This product is for research use only and shall not be used for the diagnosis or treatment of animals or humans... Read More | IRE1α kinase-IN-2 is a potent IRE1α kinase inhibitor, with an EC 50 of 0.82 µM. IRE1α kinase-IN-2 inhibits IRE1α kinase autophosphorylation (IC 50 =3.12 µM). IRE1α kinase-IN-2 inhibits XBP1 mRNA splicing in the WT cell lines.In VitroIRE1α kinase-IN-2 (compoundIRE1α kinase-IN-2 is a potent IRE1α kinase inhibitor, with an EC 50 of 0.82 µM. IRE1α kinase-IN-2 inhibits IRE1α kinase autophosphorylation (IC 50 =3.12 µM). IRE1α kinase-IN-2 inhibits XBP1 mRNA splicing in the WT cell lines.In VitroIRE1α kinase-IN-2 (compound 3) inhibits XBP1 mRNA splicing, even during ER stress. MCE has not independently confirmed the accuracy of these methods. They are for reference only.Form:Solid... Read More | Purity>98% by SDS-PAGE and HPLC analyses.FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-Purity>98% by SDS-PAGE and HPLC analyses.FunctionChemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils... Read More | Purity>98% SDS-PAGE. purified using conventional chromatography techniques.FunctionChemotactic activity for lymphocytes but not for monocytes or neutrophils.Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the Purity>98% SDS-PAGE. purified using conventional chromatography techniques.FunctionChemotactic activity for lymphocytes but not for monocytes or neutrophils.Chemokine (C motif) ligand (XCL1), as known as lymphotactin, is the only known member of the C-chemokine family and signals through the receptor XCR1, formally known as GPR5. The expression of lymphotactin is abundant in some activated T cells such as activated CD8+ T cells and other class I MHC restricted T cells. It is found in high levels in spleen, thymus, intestine and peripheral blood leukocytes, and at lower levels in lung, prostate gland and ovary. XCL1 induces its chemotactic function by binding to a chemokine receptor called XCR1. Recombinant Human XCL1 which is a single non-glycosylated polypeptide chains containing 92 amino acids and it shares approximately 60 % amino acid sequence homology with the murine and rat protein... Read More | Purity>97% by SDS-PAGE and HPLC analyses.Additional sequence informationFunction N-terminal glycine. Full-length mature chain lacking the signal peptideFunctionHas chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine Purity>97% by SDS-PAGE and HPLC analyses.Additional sequence informationFunction N-terminal glycine. Full-length mature chain lacking the signal peptideFunctionHas chemotactic activity for neutrophils. May play a role in inflammation and exerts its effects on endothelial cells in an autocrine fashion. In vitro, the processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) show a 30-fold higher chemotactic activity.Post-translationalN-terminal processed forms GRO-alpha(4-73), GRO-alpha(5-73) and GRO-alpha(6-73) are produced by proteolytic cleavage after secretion from peripheral blood monocytes... Read More |