| Description | Carboxypeptidase B, also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.This product is a recombinant carboxypeptidase B lyophilized powder Carboxypeptidase B, also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.This product is a recombinant carboxypeptidase B lyophilized powder obtained by isolation and purification from recombinant Pichia pastoris, followed by freeze - drying. It is a biochemical - grade genetic engineering enzyme, free from exogenous virus contamination, and does not contain enzyme inhibitors such as DFP, PMSF, and TLCK. It has the same activity and specificity as natural carboxypeptidase B.Product AdvantagesAnimal - free origin: No animal viruses, no pathogenic substances, no exogenous factor contamination, and high safety.High purity and high specific activity.Production scale is over 1000L.Compliance: Production equipment and production environment meet relevant regulatory requirements and conform to GMP guidelines.Complete quality documents: Relevant regulatory support documents can be provided according to customer needs.Product CharacteristicsAppearance: White or off - white powderEnzyme specific activity: ≥150 U/mg proteinMolecular weight: 36kD ± 3.6kDPurity: ≥90% (SDS - PAGE)Protein content: ≥30%Optimal pH: 7.0 - 9.0Solubility: Soluble in buffer solutionRecommended Usage MethodsPreparation of proteinDissolve the protein in a 25mM Tris - HCl solution with pH 7.6.Preparation of enzyme solutionWeigh the required enzyme powder and dissolve it in 25mM Tris - HCl (pH 7.6) to make the enzyme solution concentration 1 - 10mg/ml.ReactionRecommended enzyme amount: Target protein (mass ratio) = 1:50 - 1:1000, and the optimal pH is 7.0 - 9.0.Product ApplicationsProduction of various proteins or polypeptides, such as insulin and its analogs, beauty peptides, antimicrobial peptides, food flavor peptides, etc.Quality detection of antibodies.Determination of amino acids at the C - terminus of proteins.Storage stabilityThe lyophilized powder can be stably stored for at least 24 months at -15°C or below. After being dissolved in 25 mM Tris-HCl (pH 7.6) and stored at -15°C or below, there is no loss of activity even after 10 cycles of repeated freezing and thawing. It is recommended that the dissolved product be stored at 2–8°C for no more than 7 days, and at -15°C or below for no more than 30 days.Precautions and DisclaimerIts activity is competitively inhibited by arginine and lysine; metal ion chelators (such as EDTA) also have an inhibitory effect on the enzyme activity.This product is hygroscopic and should be equilibrated at room temperature before being taken and used.This product is for research use only and shall not be used for the diagnosis or treatment of animals or humans... Read More | 2x Taq MasterMix is a premixed system composed of Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers, and enhancers. The pre prepared PCR mixture makes the operation simpler and faster, and can minimize human error and contamination to the greatest extent possible. The original MasterMix formula 2x Taq MasterMix is a premixed system composed of Taq DNA Polymerase, Mg2+, dNTPs, PCR stabilizers, and enhancers. The pre prepared PCR mixture makes the operation simpler and faster, and can minimize human error and contamination to the greatest extent possible. The original MasterMix formula results in high yield, strong repeatability, and good stability of amplified products. This product does not contain dyes. After the PCR program is completed, an appropriate amount of sample buffer can be added as needed for electrophoresis operation. The amplified PCR product has an "A" base attached to the 3 'end, making it suitable for direct use in T/A cloning. Mainly suitable for PCR amplification of DNA, DNA sequencing and other experiments.Quality control: T665627Component5mlStorageT665627A2×Taq MasterMix5×1ml-20℃. Avoid freeze/thaw cycle.T665627BddH₂O5×1ml-20℃. Avoid freeze/thaw cycle.Notes: 2×Taq MasterMix contains Taq DNA Polymerase, 3mM MgCl2 and 400µM each dNTP After testing, there was no exogenous nuclease activity; PCR method for detecting residual DNA without host; Can effectively amplify single copy genes from multiple genomes.Usage:The following is an example of a PCR reaction system and reaction conditions for amplifying a 1 kb fragment using human genomic DNA as a template. In practical operation, corresponding improvements and optimizations should be made based on the template, primer structure, and target fragment size.1. PCR reaction systemReagent50 µlReaction systemFinal concentration2×Taq MasterMix25 µl1×Forward Primer，10 µM2 µl0.4 µMReverse Primer，10 µM2 µl0.4 µMTemplate DNA<0.5 µg<0.5 µg/50 µlddH2Oup to 50 µl/Attention: The primer concentration should be between 0.1 and 1.0 as the final concentration µ M serves as a reference for setting the range. In the case of low amplification efficiency, the concentration of primers can be increased; When non-specific reactions occur, the primer concentration can be reduced to optimize the reaction system.2. PCR reaction conditionsStepTemperatureTime/Pre denaturation95℃2 min/Denaturation94℃30 s25-35 cyclesAnneal55-65℃30 s25-35 cyclesExtend72℃30 s25-35 cyclesFinally extended72℃2 min/Attention:1) In general experiments, if the annealing temperature is 5 ℃ lower than the melting temperature Tm of the amplification primer, and the ideal amplification efficiency cannot be achieved, the annealing temperature should be appropriately reduced; When non-specific reactions occur, increase the annealing temperature to optimize the reaction conditions.2) The extension time should be set according to the size of the amplified fragment. The amplification efficiency of Taq DNA Polymerase in this product is 2 kb/min.3) The number of cycles can be set based on the downstream application of the amplification product. If the number of cycles is too small, the amplification amount is insufficient; If there are too many cycles, the probability of mismatches will increase, and non-specific backgrounds will be severe. So, while ensuring product yield, the number of cycles should be minimized as much as possible... Read More | Inquire | Format:1-ComponentEnzyme:Horseradish peroxidase | Product Characteristics UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enablesProduct Characteristics UNI-StabilPLUS is a universal stabilizer for the dilution and stabilization of both Horseradish Peroxidase (HRP) and Alkaline Phosphatase (AP) labeled proteins and antibodies, in order to maintain the molecular conformation and prevent loss of activity over time. This enables the making of pre-diluted, ready-to-use conjugates, minimizing assay errors in dilution. Superior stabilization of HRP and AP conjugated antibodies in low as well as high protein dilutions is seen, when using UNI-StabilPLUS. When tested with AP conjugated antibody stability is seen as follows: • at least 3 years at 2-8 °C • at least 2 years at room temperature • at least 4 weeks at 37 °C When tested with HRP conjugated antibody stability is seen as follows: • at least 2 years at 2-8 °C • at least 1 years at room temperature • at least 2 weeks at 37 °CUNI-StabilPLUS is recommended for the dilution of antibodies directed against rabbit immunoglobulins unlike HRP-StabilPLUS (cat. no. H494387) and Antibody Enhancer (cat. no. A494276).Composition & Properties UNI-StabilPLUS is a ready-to use buffer that appears as an opaque solution. The product is based on a mild acid Tris buffer containing proprietary stabilizing components. UNI-StabilPLUS contains neither BSA, nor other material from bovine serum, no azide, mercury or other toxic components.Working Procedure 1.Make a series of dilutions of the HRP- or AP conjugated protein in UNI-StabilPLUS in order to determine the optimal dilution. 2.Run the assay as usual or store the diluted conjugated protein preferably at 2-8 °C.Tips & Tricks • Avoid using phosphate buffers for AP-conjugated antibody assays. We recommend the use of Tris/HCl, Tween as the washing buffer, instead of a PBS buffer which will reduce signal significantly. • For extended stability of HRP conjugated antibodies, HRP-StabilPLUS (cat. no. H494387) is recommended. Handling & Storage • Store solution at 2-8 °C... Read More |