Western blotting is a commonly used analytical technique that allows researchers to separate and identify proteins from a cellular sample and confirm protein-modifications. The proteins are separated from a mixture according to their molecular weight then transferred to a support, usually a membrane/film, and the targeted protein is then marked with antibodies for visualization. Related techniques include dot blot and immunohistochemistry.
Applications of western blot include after cloning confirmation of protein production, BSE-Test for HIV diagnosis, as well as the detection of other ailments such as Lyme Disease and Hepatitis B.
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| Company | Thermo Fisher Scientific | Thermo Fisher Scientific |
| Item | Invitrogen™iBlot™ 3 Western Blot Transfer Device | Invitrogen™ Bandmate™ Automated Western Blot Processor |
| Catalog Number | IB31001 | BW1000 |
| Price | | |
| Capacity | 1 to 4 mini, or 1 to 2 midi | Up to 4 mini blots or up to 2 midi blots |
| Dimensions | 44 x 30 x 18.25 cm | 15.5 in x 24 in x 16.5 in |
| Description | The iBlot 3 Western Blot Transfer System combines an innovative, all-new robust design that delivers exceptional performance and convenience with proven iBlot technology. The iBlot 3 System is a true self-contained, plug-and-play system, with an integrated power supply and pre-packaged ready-to-use The iBlot 3 Western Blot Transfer System combines an innovative, all-new robust design that delivers exceptional performance and convenience with proven iBlot technology. The iBlot 3 System is a true self-contained, plug-and-play system, with an integrated power supply and pre-packaged ready-to-use transfer stacks. Setup, teardown, and cleanup only takes minutes compared with the much more cumbersome and hands-on steps required for wet-tank transfer.
Two separate transfer stations allow 2 users to transfer proteins independently using the same or different transfer conditions—setup, teardown and cleanup in minutes.... Read More | The Bandmate Automated Western Blot Processor is a benchtop instrument that automatically performs the hands-on steps of western blot processing, including blocking, washing, and antibody incubations. The Bandmate device requires no special reagents and is programmable to match your current western The Bandmate Automated Western Blot Processor is a benchtop instrument that automatically performs the hands-on steps of western blot processing, including blocking, washing, and antibody incubations. The Bandmate device requires no special reagents and is programmable to match your current western blotting protocols.
Bandmate Automated Western Blot Processor features include: - Fully automated antibody incubation and wash steps.
- Unique tray design that uses as little as 3.5 mL of antibody solutions for mini blots or 7.5 mL for midi blots
- Process up to 4 mini or up to 2 midi blots simultaneously
- Recoverable primary and secondary antibodies
- Adjustable, easy-to-modify parameters and protocol steps Incubation/rocking times from 1 min to 96 hours
- Dispensing volumes from 10 to 150 mL with up to 10 repetitions per step
- Rocking speeds from 5 to 40 cycles per minute
- Programmable rinse step option.
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