MALDI TOF mass spectrometers (matrix-assisted laser desorption/ionization/time-of-flight) are amongst the most popular mass spec instruments in biology. They combine two technologies, the “soft” MALDI ionization source and the TOF mass analyzer. The latter measures an ion’s mass-to-charge ratio (m/z) by measuring the time it takes to reach a detector. The former volatilizes and ionizes those molecules using a laser, a target, and an organic compound called a matrix. In MALDI, a sample (for instance, a collection of tryptic peptides) is mixed with matrix, spotted and dried (crystallized) on the surface of a metal target plate. Upon irradiation with laser light, the matrix absorbs the energy and converts it to heat, which then causes the sample to ionize and explode off the plate into the mass analyzer. MALDI TOF mass spectrometers come in two basic flavors, MALDI TOF MS and MALDI TOF/TOF MS; the latter enables tandem MS (MS/MS) studies.
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| Company | Shimadzu | Shimadzu | Shimadzu | Shimadzu | Shimadzu |
| Item | MALDImini-1 Compact MALDI Digital Ion Trap Mass Spectrometer | AXIMA-iD Plus Microorganism Analysis | iMScope QT Imaging Mass Microscope | MegaTOF Megadalton MALDI Mass Spectrometer | OmegaTOF Benchtop High Mass MALDI-TOF Mass Spectrometer |
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| Catalog Number | MALDImini-1 | AXIMA-iD Plus | iMScope QT | MegaTOF | OmegaTOF |
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| Ionization Method(s) | MALDI ion source | MALDI | Atmospheric Pressure MALDI (AP-MALDI) | MALDI | MALDI |
| Mass Analyzer | Digital Ion Trap (DIT) | MALDI-TOF MS | Q-TOF | TOF | Time-of-Flight |
| Resolution | Inquire | Inquire | Inquire | 180 to 200 kDa | >5000 FWHM – ACTH 18-39 (m/z 2465) |
| Scanning Speed | Inquire | Inquire | Inquire | 15 sec/position | Inquire |
| Mass Range | Inquire | Varies based on MALDI-TOF system used | Inquire | 0.01 to 1.5 Mda | m/z 1,000–1,500,000Da |
| Description | Inquire | Certain microorganisms can cause severe diseases and considerable damage, and need to be identified quickly and reliably. The easy-to-use AXIMA-iDPlus Microbial Identification System is the ideal tool to address these concerns. With AXIMA-iDPlus, cultured microorganisms can be directly applied to Certain microorganisms can cause severe diseases and considerable damage, and need to be identified quickly and reliably. The easy-to-use AXIMA-iDPlus Microbial Identification System is the ideal tool to address these concerns. With AXIMA-iDPlus, cultured microorganisms can be directly applied to the MALDI sample target and introduced to an AXIMA series MALDI-TOF mass spectrometer to revolutionize the daily routine in analytical and diagnostic laboratories.
Fast Response, Outstanding Recovery Rate
AXIMA-iDPlus can identify 380 clinical research samples within 5 hours in the high-throughput mode, less than one minute per sample. The results appear in a comprehensible spreadsheet format, exportable to common data processing software.
Gram-positive and gram-negative bacteria, yeast, fungi and spores are identified based on their characteristic MALDI-TOF MS fingerprints with up to 98% recovery rate down to sub-species level.
Substitutes Biochemical Standard Methods
No pre-selection of analytical methods is required; hands-on time is reduced and costs are cut dramatically.
Wide Range of Applications
AXIMA-iDPlus covers a wide range of applications in a number of industries, including diagnostics, pharmaceuticals, healthcare and food. Examples include:
- Diagnosis of human and veterinarian infectious pathogens
- Drug discovery process
- Culture collections
- Food and beverage manufacturing processes
Complementary Method
Designed for medium to high-throughput identification, AXIMA-iDPlus can be easily integrated into automated laboratory environments and any LIMS.... Read More | Inquire | The MegaTOF has been developed by optimizing the integration of a high-performance Shimadzu linear MALDI TOF mass spectrometer with a CovalX high-mass system, allowing outstanding detection of macromolecules up to 1,500 kDa.
Features - Ultrahigh sensitivity
- Detects macromolecules up to 1,500,000 Da
The MegaTOF has been developed by optimizing the integration of a high-performance Shimadzu linear MALDI TOF mass spectrometer with a CovalX high-mass system, allowing outstanding detection of macromolecules up to 1,500 kDa.
Features - Ultrahigh sensitivity
- Detects macromolecules up to 1,500,000 Da
- Stabilization of molecule using crosslinking reagent kits
- Fast, specific stabilization of protein interactions
- Also applicable for low mass applications
- Apple iPod Touch control
Applications - Antibody characterization
- Protein complexes characterization
- Therapeutic protein aggregates
- High mass MALDI imaging
- Inhibitors of protein-protein interactions
This product is only available in the United States.... Read More | The OmegaTOF system couples Shimadzu’s benchtop MALDI-TOF instrument platform (MALDI-8020) with established technology for high mass detection from CovalX Instruments Inc. The result is a user-friendly, compact platform that extends benchtop MALDI-TOF analysis to a mass range of 1.5 MegaDalton.
TheThe OmegaTOF system couples Shimadzu’s benchtop MALDI-TOF instrument platform (MALDI-8020) with established technology for high mass detection from CovalX Instruments Inc. The result is a user-friendly, compact platform that extends benchtop MALDI-TOF analysis to a mass range of 1.5 MegaDalton.
The high mass detection capabilities of the OmegaTOF allow the highest sensitivity for analyzing large biomolecules, protein interactions, peptides, oligonucleotides and polymers. This allows access to a full range of applications already developed and optimized by CovalX technologies, most notably characterizing monoclonal antibodies, such as IgG, IgA and IgM. Crosslinking chemistries developed by CovalX enable the OmegaTOF to measure intact protein interactions directly, providing insight into protein aggregation, mAb:antigen interactions, protein complex stoichiometry or ligand association/dissociation measurements.
The OmegaTOF offers: - 200 Hz solid-state 355 nm laser featuring a class-leading two billion shots per lifetime
- Fast sample introduction using a load-lock chamber and quick sample stage movement
- Integrated barcode reader
- Patented TrueClean™ source cleaning
WideBore™ ion optics technologies that significantly reduce contamination from desorbed MALDI samples - Ease-of-use
- Low maintenance
- Small footprint
- Quiet operation at <55 dB
OmegaTOF data can be directly imported into the latest versions of CovalX ComplexTracker software for protein interaction analysis. Tailored MALDI Solutions acquisition software features data security for regulatory compliance facilitated with password-controlled user login, encrypted data, and user traceability. ... Read More |
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