| Description | Light sheet fluorescence microscopy with the UltraMicroscope II enables you to visualize whole biological systems and allows the generation of sharp and bright 3D images. Whether imaging large tissue samples or even entire organs, you are equipped for the task at hand regardless of the clearing Light sheet fluorescence microscopy with the UltraMicroscope II enables you to visualize whole biological systems and allows the generation of sharp and bright 3D images. Whether imaging large tissue samples or even entire organs, you are equipped for the task at hand regardless of the clearing protocol and imaging solution being used. This light sheet microscope comes in two configurations: it can be fitted with either the Zoom Body Module for easy handling in a multi-user environment, or the Super Plan Module that can deliver never-before-seen image resolution and quality.
Features:
- From overview to cellular imaging: Visualize entire biological systems like whole rodent organs, mouse embryos or insect larvae, and tumors at cellular resolution.
-
High flexibility: The UltraMicroscope II can be tailored to your needs with our modular light sheet technology and a series of objective lenses specially designed for high resolution light sheet microscopy.
-
Easy adaptation: Apply any clearing protocol and make use of the automated refractive index compensation to easily maintain high-quality images.
-
User friendly: An intuitive design and short training time make this light sheet microscope the perfect tool for multi-user environments.
... Read More | The MACSima™ Platform, our revolutionary multiomics solution, streamlines your spatial biology research from end to end, reducing experimental workload and generating impactful data in no time. The platform's core, the fully automated MACSima System, combines high-plex spatial protein profiling withThe MACSima™ Platform, our revolutionary multiomics solution, streamlines your spatial biology research from end to end, reducing experimental workload and generating impactful data in no time. The platform's core, the fully automated MACSima System, combines high-plex spatial protein profiling with RNA detection on the same section, pushing the boundaries of what’s possible. The MACSima Platform empowers researchers to make pioneering discoveries by facilitating the identification of novel cell types, understanding their interactions, and exploring their impact on the tissue microenvironment, thereby advancing biomarker identification, drug discovery, and the development of precision immunotherapy.
Highlights:
- Same-section multiomics – Gain a deeper understanding of the causes and consequences of biological events by connecting phenotype with gene expression. Analyze hundreds of protein markers and dozens of RNAs within a single tissue section.
-
High-plex spatial profiling of proteins and RNA – Use MICS (MACSima Imaging Cyclic Staining) technology to analyze hundreds of protein markers and dozens of RNAs within a single sample in one run. Whether you choose a compact panel of 15-20 protein markers or delve into extensive panels encompassing up to 200+ protein markers and 24 RNAs, the possibilities are vast.
- Comprehensive spatial biology platform – Count on the MACSima Platform for a complete and harmonized solution, featuring instrument, sample carriers, antibodies pretested for MICS technology, and analysis software. Miltenyi Biotec stands out as the sole provider offering this all-embracing package.
- Antibodies for highest convenience, flexibility, and reproducibility – Take advantage of the world's largest ultra-high-plex antibody portfolio for spatial biology, targeting 600+ biomarkers. Enjoy complete flexibility by choosing individual antibodies or ready-to-use 96-well plates with antibody panels. With REAscreen™ Design, you can even have personalized panels for the highest standardization, whether you require a large stock of a 30-marker panel or just a few replicates of a 100-marker panel.
- RNAsky™ Detection Probes for spatial transcriptomics – Explore RNAs alongside proteins on the same section. Offered in predefined or customizable panels, these probes unlock new targets undetectable by antibodies, like cytokines.
- Automated processing and imaging – Plan your experiment and leave the execution to the MACSima System.
- Analyze any kind of fixed sample – Image all types of fixed samples, whether tissue or single cells, and perform any analysis you can imagine.
- Intuitive analysis – Unlock the full potential of your spatial data with the MACS® iQ View Analysis Software. Its intuitive design makes analyzing vast spatial biology experiment data accessible to users of all backgrounds, enabling quick generation of conclusive and publication-ready results. It features an intuitive user interface and offers many analysis options (t-SNE/UMAP plots, heatmaps, distance mapping, …).
- Technical support and trusted scientific partnership – Receive comprehensive support from a team of field application specialists, spanning the entire spectrum – from panel design and experimental setup to data analysis. Miltenyi Biotec uses the MACSima Platform in its own research initiatives, demonstrating a profound understanding of scientists' needs and fostering partnerships on eye level.
... Read More | The UltraMicroscope Blaze, the market's most automated light sheet microscope, is ideal for high-resolution 3D imaging of large and multiple cleared samples. With the revolutionary Lightspeed mode and the new image processing software for 3D large volumes, MACS iQ View, it sets new benchmarks for The UltraMicroscope Blaze, the market's most automated light sheet microscope, is ideal for high-resolution 3D imaging of large and multiple cleared samples. With the revolutionary Lightspeed mode and the new image processing software for 3D large volumes, MACS iQ View, it sets new benchmarks for speed and image quality. Offering both a comprehensive 3D overview of the entire sample and a detailed analysis down to the subcellular level, it's a versatile tool for imaging organoids, rodent organs, or samples as large as entire mouse models or human kidneys. Used across fields like neuroscience, immuno-oncology, and developmental biology, the UltraMicroscope has contributed to over 900 publications, attesting to its excellence in generating valuable research data.
Features:
- Fully automated - Automated objective exchanger, autofocus, automatic sample release, automatic chromatic correction in both excitation and detection, as well as a batch measurement mode for imaging multiple samples are included.
- Fast - The LightSpeed mode in the UltraMicroscope Blaze offers high-resolution imaging at an unparalleled speed, up to 60 times faster than its predecessor and other systems. This remarkable speed is evident in its capability to capture a full 3D image of an entire mouse brain in less than 3 minutes, a process that would take several hours with other light sheet microscopes. The LightSpeed mode is not only fast but it also provides the best possible image quality and resolution.
- Easy to use - With the UltraMicroscope Blaze, anyone can become a light sheet microscopist. There is no need for technical expert knowledge.
- Flexible - Handling all clearing media, from water to organic solvents thanks to the dedicated objective dipping caps.
- Cutting-edge light sheet optics - Up to six light sheets, optimized for uniform illumination, improved optical sectioning, and high image quality, are combined with dedicated high numerical aperture (NA) and long working distance immersion objectives, ensuring the most homogeneous imaging conditions.
- Image processing software - Achieve superior image quality through the user-friendly post-processing workflow of "MACS® iQ View – 3D Large Volume," which includes 3D cropping, destriping, denoising, deconvolution, stitching, and contrast compression. Specifically designed for the UltraMicroscope Blaze, this software eliminates the necessity for data conversion. Utilize the convenience of batch processing to load, queue, and process multiple datasets in a single run. This feature is particularly valuable for large and multiple datasets acquired with the LightSpeed mode.
- Complete workflow - Miltenyi Biotec has got you covered from A to Z, with validated 3D-IF antibodies for staining large samples, the MACS® Clearing Kit for tissue clearing, easy-to-follow immunostaining and tissue clearing protocols, and smart software solutions, from data acquisition to image processing.
- 3D printed sample holders with clamping mechanism - Choose adaptable and easy-to-use 3D printed holders that fit your biological samples. The clamping mechanism allows for the stable holding of samples during fast acquisitions. These sample holders are highly recommended in combination with our LightSpeed mode.
- Support and trusted scientific partnership - Receive comprehensive support from a team of specialists, spanning the entire spectrum, from staining and clearing to imaging and image processing. Miltenyi Biotec employs the UltraMicroscope Platform in its own research initiatives, demonstrating a profound understanding of scientists' needs and fostering partnerships on an equal footing.
... Read More |
| Light Source | Laser module (max. 5 laser lines): 405 nm, 488 nm, 561 nm, 639 nm, 785 nm
OR Supercontinuum white light laser: 460 to 800 nm | Six high-power excitation LEDs (filters: 386/23 nm, 420/10 nm, 470/40 nm, 531/46 nm, 628/32 nm, 725/40 nm) | Laser beam combiner (max. 5 laser lines with 405, 488, 561, 639, 785 nm, 50 mW–100 mW per diode) or supercontinuum white light laser (405–850 nm, 1–4 mW/nm). |