Flow cytometers are instruments used to analyze cells within a solution and provide multi-parameter analysis capabilities. A flow cytometer instrument is often comprised of a fluidic system to transport cells in solution, lenses and filters, as well as a laser or other light source to provide scattered and fluorescent light that is then analyzed by a detector. Flow cytometer detectors commonly include photodiodes or photomultiplier tubes. Used extensively throughout the life sciences industry, flow cytometers provide users with a wealth of information such as the determination of cell characteristics and function, biomarker discovery, treatment and diagnosis information, cell counting and or sorting, and many others. When comparing flow cytometers, ensure that the excitation wavelength and flow rate are compatible with your specific assay. Additionally, many flow cytometers can accommodate several sample formats such as tubes or microplates, and should be considered to ensure smooth integration with your current processes.
Find, compare, and request a quote for flow cytometers across leading suppliers at Labcompare; including access to product citations, related published figures, and first-hand reviews from our scientific community.
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| Company | Thermo Fisher Scientific | Thermo Fisher Scientific |
| Item | Invitrogen™ Attune™ Flow Cytometers | Bigfoot™ Cell Sorter |
| Citations | | |
| Figures | | |
| Catalog Number | Attune Cytpix / Attune NxT | 4 Laser Conventional PL00304 / 4 Laser Conventional PL00307 / 5 Laser Conventional PL00303 / 5 Laser Spectral PL00302 / 6 Laser Spectral IR option PL00301 / 6 Laser Spectral 445 nm PL00300 / 7 Laser Spectral PL00299 / 9 Laser Spectral PL00285 |
| Price | | |
| Flow Rate | Up to 1000µl/min | Inquire |
| Applications | All flow cytometry applications + brightfield imaging (Cytpix) | Inquire |
| Description | Attune Flow Cytometers are compact benchtop cell-analyzers that can be configured with up to 4 spatially-separated lasers to flexibly design, run, and analyze panels of up to 14 colors. Uniquely powerful acoustic technology avoids clogging, and dramatically reduces cell preparation and processing Attune Flow Cytometers are compact benchtop cell-analyzers that can be configured with up to 4 spatially-separated lasers to flexibly design, run, and analyze panels of up to 14 colors. Uniquely powerful acoustic technology avoids clogging, and dramatically reduces cell preparation and processing times, even for troublesome large cells and dissociated tissue masses. The systems attain sample through-put rates of 1,000 µL/min, 10x times faster than traditional cytometers without compromising data integrity. Attune Cytometric Software makes the system easy-to-use with an optional module for 21 CFR part 11 compliance.
The Attune CytPix model includes a high-speed brightfield camera to capture images of cell populations as you acquire flow data. Combined with integrated image processing software to measure over 25 derived image parameters, the Attune CytPix enables researchers to correlate morphology data with flow cytometric parameters and gain novel insights into their sample biology. Gates can be adjusted to exclude aggregates, coincident events, debris, and unwanted cells.
Features of the Attune CytPix Flow Cytometer include: - Sample flow rates up to 1000ul/min
- 35,000 event/sec of flow cytometry data
- Up to 4 spatially separated lasers (14 fluorescence detectors + SSC + FSC)
- Volumetric sample syringe for accurate particle counts without counting beads
- Imaging of up to 6000 events/sec
- More than 25 label-free image-derived parameters to inform gating strategy
- Optional autosamplers and robotic automation for high-throughput plate sampling
Attune NxT Laser configuration SKU's
A24858 (blue/red/yellow/violet) / A24859 (blue/violet/yellow) / A24860 (blue/red/violet) / A24861 (blue/yellow) / A24862 (blue/violet) / A24863 (blue/red) / A24864 (blue) / A28993 (blue/red/yellow) / A28995(blue/green) / A28997 (blue/green/red) / A28999(blue/green/violet) / A29001(blue/red/violet/green) / A29002(blue/violet(6)) / A29003(blue/red/violet(6))
Attune CytPix Laser configuration SKU's
A51840 (blue/red) / A51841 (blue/violet) / A51842 (blue/yellow) / A51843 (blue/violet(6)) / A51844 (blue/red/violet) / A51845 (blue/red/yellow) / A51846 (blue/violet/yellow) / A51847 (blue/red/violet/yellow) / A51849 (blue/red/yellow/violet(6))
Accessories
- A42901 (CytKick)
- A42973 (CytKick Max)
- A28006 (External Fluid Supply)
- 100022779 (Attune NxT Yellow Laser Upgrade Kit)
- 100022778 (Attune NxT Red Laser Upgrade Kit)
- A32701 (Attune NxT Green Laser Upgrade Kit)
... Read More | The Invitrogen Bigfoot Spectral Cell Sorter is designed to extend your reach while fitting into your workflow. With up to nine lasers and 60 detectors, you can migrate your existing experiments using conventional compensation or convert to spectral analysis to resolve even more markers and cell The Invitrogen Bigfoot Spectral Cell Sorter is designed to extend your reach while fitting into your workflow. With up to nine lasers and 60 detectors, you can migrate your existing experiments using conventional compensation or convert to spectral analysis to resolve even more markers and cell populations. Integrated biocontainment and aerosol management help protect you and your samples. With sorting speeds in excess of 70,000 events per second, the system can sort a 96-well plate in less than 8 seconds and a 384-well plate in less than 11 seconds . Simple enough to work in a solo lab and powerful enough for a major lab, the Bigfoot Spectral Cell Sorter will help you master the full range of sorting experiments, from fluorescent protein-tagged cell sorting to deep immunophenotyping , genomics, cell therapy and gene therapy research and other high-throughput applications.
Features - High performance and throughput
- Integrated biocontainment and aerosol management
- Choice of conventional compensation or spectral analysis
- Automated and wizard driven software for ease of use
- Flexibility to support a range of cell types and applications
- Streamlined design
- Lab or core facility friendly
... Read More |
| Excitation Wavelength | 405 nm, 488 nm, 561 nm, 532 nm, and 637 nm | 349 nm, 405 nm, 445 nm, 488 nm, 532 nm, 561 nm, 594 nm, 640 nm, 785 nm |
| Throughput | Throughput rates of 12.5 µL/min or 1,000 µL/min and acquisition speeds of 35,000 events per second | >70,000 EPS |
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