Single cell dispensers are specialized laboratory instruments used to isolate and dispense single cells for various analytical techniques. Often utilizing a combination of microscopy and microfluidics techniques, single cell dispensers can isolate stained or unstained cells and gently dispense cells into various-sized well plates for analysis. Single cell dispensers are an indispensable instrument for streamlining cell line development (CLD) by utilizing automation while increasing reproducibility and efficiency while ensuring monoclonality. Single cell dispensers ensure monoclonality in several different ways and the methods used should be compared to ensure compliance with your specific assay. Plate formats should also be considered as these can vary from instrument to instrument and most instruments can accommodate multiple plate sizes. Plate sizes can also affect analysis time, with higher capacity plates leading to longer analysis times. Lastly, the size of your cells should be considered, single cell dispensers are often compatible with a range of cell sizes but compatibility with your specific cell sizes should be ensured.
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| Company | CYTENA | CYTENA | CYTENA | CYTENA | CYTENA |
| Item | C.SIGHT™ Single Cell Isolation | B.SIGHT™ Microbial Single Cell Isolation | C.SIGHT™ 2.0 Single Cell Isolation | F.SIGHT™ Single Cell Dispenser with Fluorescent Sorting | UP.SIGHT™ Single Cell Dispenser and Plate Imager |
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| Catalog Number | C.SIGHT | B.SIGHT | C.SIGHT 2.0 | F.SIGHT | CY.US.UCA.233 |
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| Description | Label-free single-cell isolation for cell line development.
The C.SIGHT™ uses CYTENA’s validated single-cell dispensing technology to make isolating individual mammalian cells more efficient. The device offers image-based proof of clonality and provides efficient single-cell seeding all within a Label-free single-cell isolation for cell line development.
The C.SIGHT™ uses CYTENA’s validated single-cell dispensing technology to make isolating individual mammalian cells more efficient. The device offers image-based proof of clonality and provides efficient single-cell seeding all within a compact benchtop instrument – no staining or labeling required. The C.SIGHT also features intuitive software with an interactive well plate that enables you to customize your experiments.
Key features and benefits: - Brightfield imaging
- Gentle single-cell dispensing
- Small footprint & benchtop
- Cost efficient
... Read More | The second generation of B.SIGHT is a microbial isolation platform that uses cutting-edge optical microscopy and our single-cell dispensing technology to enable the isolation of the smallest microbial cells. This device revolutionizes microbiology research by allowing rapid and efficient isolation The second generation of B.SIGHT is a microbial isolation platform that uses cutting-edge optical microscopy and our single-cell dispensing technology to enable the isolation of the smallest microbial cells. This device revolutionizes microbiology research by allowing rapid and efficient isolation of bacteria from complex microbiome samples or transformed yeast and/or bacteria cultures used for strain development.
Key features or benefits: - Fast high-throughput single- cell isolation and cloning of microorganism
- Isolate single bacteria/yeast in a 384-well plate in under 8 minutes
- Precise deposition of single cells onto PCR plates
- Assurance of clonality
- Can be integrated into automation workflows
... Read More | The C.SIGHT 2.0 is the latest iteration of our single-cell dispensing system designed to optimize cell line development (CLD) applications. With faster single-cell isolation processing times and our new EASY.ON cartridges for contamination-free dispensing, the C.SIGHT 2.0 provides an easy entry The C.SIGHT 2.0 is the latest iteration of our single-cell dispensing system designed to optimize cell line development (CLD) applications. With faster single-cell isolation processing times and our new EASY.ON cartridges for contamination-free dispensing, the C.SIGHT 2.0 provides an easy entry point into the world of single-cell handling. The C.SIGHT 2.0’s compact design makes it possible to operate the system in biosafety cabinets, enabling single-cell isolation in sterile conditions.
Key features and benefits: - Fast and efficient single-cell dispensing
- No risk of contamination
- Assurance of clonality
- Cell Focusing technology
- Easy setup
- Compact design
... Read More | Fluorescent and label-free single-cell isolation for cell line development.
The F.SIGHT makes single-cell dispensing simpler and more efficient with our state-of-the-art fluorescent sorting. Design your individual fluorescence experiments within minutes with the option to use bright field imagingFluorescent and label-free single-cell isolation for cell line development.
The F.SIGHT makes single-cell dispensing simpler and more efficient with our state-of-the-art fluorescent sorting. Design your individual fluorescence experiments within minutes with the option to use bright field imaging for non-fluorescent cells. The F.SIGHT records and saves full-resolution bright field, fluorescence, and overlay images for future analysis and proof of clonality, giving you peace of mind that your results are accurate and comprehensive.
Key features and benefits: - Adjustable laser
- Detection of low-intensity dye or low copy numbers
- Laser for red fluorescence available (mCherry, Texas Red, RFP, Deep Red, etc.)
... Read More | The ultimate upgrade in cell line development.
The UP.SIGHT™ streamlines cell line development (CLD) workflows by automating labor-intensive and time-consuming steps. We have combined our patented, highly efficient, fast and gentle single-cell dispensing technology with a superfast, high-quality The ultimate upgrade in cell line development.
The UP.SIGHT™ streamlines cell line development (CLD) workflows by automating labor-intensive and time-consuming steps. We have combined our patented, highly efficient, fast and gentle single-cell dispensing technology with a superfast, high-quality imaging system that provides a full well image without image stitching. The UP.SIGHT offers two independent methods of assuring monoclonality: first, images are taken at the dispensing nozzle to ensure only one cell is placed in each well and nonclonal droplets are discarded; second, the single cells are confirmed once more with 3D Full Well Imaging, a new and innovative method that images the full volume of each well from the point of dispensing using the integrated imager, leading to a probability of clonality >99.99%.
Key features and benefits: - Double assured clonality
- Minimal cell loss
- Small footprint and benchtop
... Read More |
| Plate Formats | 96- and 384-well plates | 96,384-well plates | SBS format: 96-, and 384-well plates | 96- and 384-well plates | 96 or 384-well plates |
| Applications | Cell line development, Single-cell omics, Cell therapy, Gene therapy, Low-volume assays (e.g., Whole Genome Amplification, PCR) | Synthetic biology, Single bacteria isolation, Single yeast isolation, Microbiome research, Anaerobic microorganism isolation | Cell line development, iPSC isolation for clonal culture, Cell therapy, Gene therapy | Cell line development, Gene therapy, Rare cell research and discovery, Monoclonal cell culture | Monoclonal cell culture, Monoclonal antibody development, Automated cell line development, Cell therapy & stem cell research, Gene Therapy |
| Volume | ~200 pL | ~50 pL | ~200 pL | ~200 pL | Inquire |
| Analysis Time | Single-cell dispensing 96-well plate: ~ 5 min
Single-cell dispensing 384-well plate: ~ 15 min | Single-cell dispensing 96-well plate: ~ 3 min
Single-cell dispensing 384-well plate: ~ 8 min | Single-cell dispensing 96-well plate: ~ 2 min
Single-cell dispensing 384-well plate: ~ 8 min | Single-cell dispensing 96-well plate: ~ 5 min
Single-cell dispensing 384-well plate: ~ 15 min | Single-cell dispensing into 96-well plate: ~2 min
Single-cell dispensing into 384-well plate: ~8 min
Single-cell dispensing plus 3D Full Well Imaging 384-well plate: ~25 min
Well bottom imaging of 96-well plate: ~8 min
Well bottom imaging of 384-well plate: ~8 min
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| Dimensions | (WxDxH) 600 x 400 x 240 mm | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 635 x 400 x 282 mm | (WxDxH) 600 x 400 x 240 mm | (WxDxH) 635 x 400 x 282 mm |
| Cell size range | 5 to 40 µm | 0.5 to 20 µm | Up tp 40 µm | 5 to 40 µm | 5 to 40 µm |
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