Spectrophotometers are used to measure the concentration of a known substance in a solution. They do this by passing a light through the substance and measuring light intensity as a function of wavelength. Either single beam or double beam spectrophotometers can be purchased. In a single beam spectrophotometer, a reference standard is used to measure light intensity before and after the sample is loaded. For double beam spectrophotometers the beam is split with one beam passing through the standard and the other through the sample to compare intensities. While single beam spectrophotometers are typically less expensive and have higher sensitivity, double beam spectrophotometers tend to be more stable and easier to use. Spectrophotometers are important pieces of equipment in chemistry, physics, biochemistry, clinical, and materials testing labs.
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| Company | Agilent Technologies | Agilent Technologies |
| Item | Cary 6000i UV-Vis-NIR Spectrophotometer | Cary Eclipse Fluorescence Spectrophotometer |
| Reviews | | |
| Catalog Number | 10079400 | 10075200 |
| Price | | |
| Type | UV-VIS-NIR | Fluorescence spectrophotometer |
| Wavelength Range | 175 to 1800 nm | 200–900 nm |
| Wavelength Accuracy | UV/vis +/- 0.1, NIR +/- 0.2 | ± 1.5 nm |
| Scanning Speed | UV-Vis 2,000 nm/min; NIR 8,000 nm/min | 24,000 nm/min |
| Spectral Bandwidth | UV-Vis 0.01 - 5.00 nm; NIR 0.04 - 20 nm | Excitation: 1.5, 2.5, 5, 10, 20 nm and 10 nm round. Emission: 1.5, 2.5, 5, 10, 20 nm and 10 nm round |
| Optical System | Double out-of-plane Littrow monochromator | Czerny-Turner monochromator |
| Light Source | Mercury lamp | Xenon Flash Lamp |
| Resolution | UV/Vis less than 0.05; NIR less than 0.1 | Inquire |
| Description | The Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, The Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, the Cary 6000i is the ultimate tool for materials characterization spectroscopy.
Using Cary WinUV software with the Cary 6000i makes it easy to perform powerful analysis and control optional accessories. The large sample compartment can be expanded to hold large accessories and integrating spheres for spectral and diffuse reflectance. The LockDown mechanism makes it possible to quickly change and position accessories for reproducible results.
Features
- Measure beyond 8.0 absorbance units with reference beam attenuation
- Measure 175 to 1800 nm using a narrow-band InGaAs detector and 600 l/mm NIR diffraction gratings for unmatched sensivity in the short-wave infrared
- WinUV software—modular software with power analysis and enhanced transfer and report export capabilities
- Versatile set of accessories for materials research including spectral and diffuse reflectance
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with LockDown mechanism for quick changes and positioning for reproducible results
- Maximum light throughput using Schwarzchild coupling optics for higher accuracy at low transmission levels
- Minimal noise and stray light using a floating aluminum casting and double out-of-plane Littrow monochromator
- Extended dynamic range by attenuating the reference beam to balance double beam signal levels.
- Nitrogen purging—separate purging in the sample and monochromator compartments to reduce oxygen and water vapor in the ultra-violet.
... Read More | The Agilent Cary Eclipse fluorescence spectrophotometer is sensitive, accurate, and flexible. Ideal for use as a routine fluorometer, the Cary Eclipse can easily be switched between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Additional flexibility can be The Agilent Cary Eclipse fluorescence spectrophotometer is sensitive, accurate, and flexible. Ideal for use as a routine fluorometer, the Cary Eclipse can easily be switched between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Additional flexibility can be achieved through various accessories, including temperature control and solid sample handling, automatic polarizers, and a microplate reader for high sample throughput.
The Cary Eclipse is ideal for fluorescent measurements in life sciences and biotechnology, with high sensitivity and inherent bleaching protection for photosensitive samples. Fast data collection enables kinetics measurements, while fiber optics allow for remote sample measurements.
Features - Flexible fluorescence spectrometer with multiple data collection modes including fluorescence, phosphorescence, chemiluminescence, bioluminescence, and time resolved phosphorescence.
- Robust and reliable performance backed up by a 10-year lamp life guarantee (for new Cary Eclipse systems purchased from Agilent or participating partners).
- Low cost-of-ownership due to the guaranteed long lifetime of the Cary Eclipse fluorometer lamp. This also eliminates the need for lamp replacements, a time-consuming and hazardous waste-producing technical operation.
- Easy-to-use Cary WinFLR software suitable for novice and expert users alike. Easily switch between measurement modes without touching the hardware, and quickly get reliable, accurate results.
- Room light immunity provided by a unique design allows the instrument to operate with the front and top panels removed—for easy access to samples and fast installation of large accessories, expanding analytical capabilities.
- Performance and sensitivity that you need right across the fluorescence spectrum due to high-sensitivity optics and detectors.
- High throughput laboratories and fast kinetics applications are well suited to the Cary Eclipse, as its xenon flash lamp allows the instrument to capture a data point every 12.5 ms, and scan at 24,000 nm/min without peak shifts.
- Sampling flexibility enables a wide range of fluorescence applications, with accessories available for every sample type—liquids, solids, and powders. Polarizers and temperature control are also available.
- Inherent protection from photodegradation, as the Cary Eclipse only illuminates the sample when data is actually being acquired. This protects your valuable samples so you can measure them repeatedly and get the same, reliable results.
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| Quantity | EA | EA |
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