Spectrophotometer

Spectrophotometers are used to measure the concentration of a known substance in a solution. They do this by passing a light through the substance and measuring light intensity as a function of wavelength. Either single beam or double beam spectrophotometers can be purchased. In a single beam spectrophotometer, a reference standard is used to measure light intensity before and after the sample is loaded. For double beam spectrophotometers the beam is split with one beam passing through the standard and the other through the sample to compare intensities. While single beam spectrophotometers are typically less expensive and have higher sensitivity, double beam spectrophotometers tend to be more stable and easier to use. Spectrophotometers are important pieces of equipment in chemistry, physics, biochemistry, clinical, and materials testing labs.

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CompanyAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent TechnologiesAgilent Technologies
ItemCary 3500 Compact UV-Vis SpectrophotometerCary 4000 UV-Vis SpectrophotometerCary 5000 UV-Vis-NIR SpectrophotometerCary 6000i UV-Vis-NIR SpectrophotometerCary Eclipse Fluorescence Spectrophotometer
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Catalog NumberCary 3500 Compact10079200100793001007940010075200
Price
TypeUV-Vis double beam spectrophotometerUV-VisUV-VIS-NIRUV-VIS-NIRFluorescence spectrophotometer
Wavelength Range190 to 1100 nm175 to 900 nm175 to 3300 nm175 to 1800 nm200–900 nm
Wavelength Accuracy± 0.2 nmInquireInquireUV/vis +/- 0.1, NIR +/- 0.2± 1.5 nm
Scanning SpeedUV-Vis 150,000 nm/minUV-Vis 2,000 nm/minUV-Vis 2000 nm/min; NIR 8000 nm/minUV-Vis 2,000 nm/min; NIR 8,000 nm/min24,000 nm/min
Spectral Bandwidth0.1 to 5 nmUV-Vis 0.01 - 5.00 nmDown to 0.01 nmUV-Vis 0.01 - 5.00 nm; NIR 0.04 - 20 nmExcitation: 1.5, 2.5, 5, 10, 20 nm and 10 nm round. Emission: 1.5, 2.5, 5, 10, 20 nm and 10 nm round
Optical SystemDouble out-of-plane Littrow monochromatorDouble BeamDouble BeamDouble out-of-plane Littrow monochromatorCzerny-Turner monochromator
Light SourceXenon flash lampTungsten halogen visible and deuterium arc UVTungsten halogen visible and deuterium arc UVMercury lampXenon Flash Lamp
Resolution0.1 nm limiting resolutionInquireInquireUV/Vis less than 0.05; NIR less than 0.1Inquire
DescriptionThe Cary 3500 Compact UV-Vis is a double-beam spectrophotometer featuring new and unique measurement capabilities that can be deployed in regulated environments. The system performs ambient and temperature-controlled experiments with air-cooled Peltier temperature control, and it accurately analyzes... Read MoreThe Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.

Controlled by the Cary WinUV software,
... Read More
The Cary 5000 is a high-performance UV-Vis and NIR spectrophotometer with superb photometric performance in the 175–3300 nm range. Using a PbSmart detector, the Cary 5000 extends its NIR range to 3300 nm making it a powerful tool for materials science research. The Cary WinUV software, a modular ... Read MoreThe Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, ... Read MoreThe Agilent Cary Eclipse fluorescence spectrophotometer is sensitive, accurate, and flexible. Ideal for use as a routine fluorometer, the Cary Eclipse can easily be switched between fluorescence, phosphorescence, chemiluminescence, and bioluminescence measurements. Additional flexibility can be ... Read More
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