A UV-Vis spectrophotometer is used to determine the absorption of light from a sample and can be used as a detector for HPLC. A sample is placed in the UV/VIS beam and absorbance versus wavelength is measured. A double beam spectrophotometer utilizes two beams of light: a reference beam and a sampling beam that passes through the sample. Some double beam spectrophotometers have two detectors that allow the two beams to be measured at one time.
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| Company | Agilent Technologies | Agilent Technologies | Agilent Technologies | Agilent Technologies |
| Item | Cary 3500 Flexible UV-Vis Spectrophotometer | Cary 4000 UV-Vis Spectrophotometer | Cary 5000 UV-Vis-NIR Spectrophotometer | Cary 6000i UV-Vis-NIR Spectrophotometer |
| Reviews | | | | |
| Catalog Number | Cary 3500 Flexible | 10079200 | 10079300 | 10079400 |
| Price | | | | |
| Type | UV-Vis double beam spectrophotometer | UV-Vis | UV-VIS-NIR | UV-VIS-NIR |
| Wavelength Range | 190 to 1100 nm | 175 to 900 nm | 175 to 3300 nm | 175 to 1800 nm |
| Wavelength Accuracy | ± 0.2 nm | Inquire | Inquire | UV/vis +/- 0.1, NIR +/- 0.2 |
| Scanning Speed | UV-Vis 150,000 nm/min | UV-Vis 2,000 nm/min | UV-Vis 2000 nm/min; NIR 8000 nm/min | UV-Vis 2,000 nm/min; NIR 8,000 nm/min |
| Spectral Bandwidth | 0.1 to 5 nm | UV-Vis 0.01 - 5.00 nm | Down to 0.01 nm | UV-Vis 0.01 - 5.00 nm; NIR 0.04 - 20 nm |
| Light Source | Xenon flash lamp | Tungsten halogen visible and deuterium arc UV | Tungsten halogen visible and deuterium arc UV | Mercury lamp |
| Resolution | 0.1 nm limiting resolution | Inquire | Inquire | UV/Vis less than 0.05; NIR less than 0.1 |
| Description | The Cary 3500 Flexible UV-Vis is a double-beam spectrophotometer with superior photometric performance—for challenging measurements with minimal sample preparation—that can be deployed in regulated environments. The system offers a uniquely large sample compartment with a small footprint for The Cary 3500 Flexible UV-Vis is a double-beam spectrophotometer with superior photometric performance—for challenging measurements with minimal sample preparation—that can be deployed in regulated environments. The system offers a uniquely large sample compartment with a small footprint for analyzing liquid samples that require long-length cuvettes, as well as characterizing solid samples. A xenon flash lamp—complete with a 10-year replacement warranty (for Cary 3500 instruments purchased from Agilent or participating partners)—eliminates the daily warm-up time and drastically reduces the frequency and cost of lamp replacement.
The Cary 3500 comes complete with Cary UV Workstation software—a next-generation analytical platform with streamlined methods for wavelength reads, scanning, concentration, kinetics, and temperature measurements. When configured with Agilent OpenLab data management software, Cary UV Workstation data is managed, protected, and stored in a local or central secure database.
Features
- Measure liquid samples via unique variable pathlength rectangular and cylindrical cell holders, which enable quick and reproducible pathlength changes up to 10 cm. This design is toolless and eliminates time-consuming alignment procedures.
- Handle a wide variety of solid samples and thin films with a large sample compartment that accommodates most sample sizes while minimizing its footprint on the lab bench.
- Ensure you never miss a data point—even in rapid reaction kinetics measurements—with advanced xenon flash lamp technology achieving a data collection rate of 250 points per second.
- Achieve research-grade photometric performance, stability, and high linear dynamic range with minimal sample preparation.
- Reduce data processing time and calculation errors with more than 50 built-in and customized calculations that can be saved and applied automatically to any method used routinely.
- Be up and running in minutes by following the intuitive video tutorials in Cary UV Workstation’s Help and Learning Center.
- Meet data integrity standards—provides the technical controls to acquire securely, process, report, and store data in laboratories that must follow the compliance guidelines of FDA 21 CFR Part 11, EU Annex 11, GAMP5, as well as ISO/IEC 17025 and EPA’s 40 CFR Part 160.
- Store and manage your Cary 3500 Flexible UV-Vis spectrophotometer data in a local secured storage on the workstation PC, or in a central storage location.
- Deploy the OpenLab software suite and get optional secure database storage for electronic records, complete user administration and access privileges, software licensing, and security settings—e-signature workflow and enhanced audit trail review capabilities.
... Read More | The Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.
Controlled by the Cary WinUV software,The Cary 4000 is a high-end UV-Vis spectrometer with excellent photometric performance in the 175–900 nm range, making it a powerful reference spectrometer. The system is ideal for analyzing solid samples or biotechnology samples with minimal sample preparation.
Controlled by the Cary WinUV software, a Windows-based modular software, it can be ordered with specific modules for bio and pharma.The UV-Vis spectrometer has a number of optional accessories for liquid samples, like temperature control and multicell holders.
Features
- Measure beyond 8.0 absorbance units with reference beam attenuation
- WinUV software—a variety to applications from scanning to kinetics and thermal
- Versatile suite of accessories including temperature control, liquid and solid sample holders, solids autosamplers, multicell holders, and diffuse and specular reflectance accessories
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with precision LockDown mechanism for quick changes of accessories
- Maximum light throughput using Schwarzchild coupling optics
- Minimal noise and stray light using a floating aluminum casting and double out-of-plane Littrow monochromator
- Extended dynamic range by attenuating the reference beam to be more equivalent to the samples absorbance
- Nitrogen pumping—separate purging in the sample and monochromator compartments
... Read More | The Cary 5000 is a high-performance UV-Vis and NIR spectrophotometer with superb photometric performance in the 175–3300 nm range. Using a PbSmart detector, the Cary 5000 extends its NIR range to 3300 nm making it a powerful tool for materials science research. The Cary WinUV software, a modular The Cary 5000 is a high-performance UV-Vis and NIR spectrophotometer with superb photometric performance in the 175–3300 nm range. Using a PbSmart detector, the Cary 5000 extends its NIR range to 3300 nm making it a powerful tool for materials science research. The Cary WinUV software, a modular Windows-based software, makes it easy to perform powerful analysis and control a number of optional accessories.
The large sample compartment can be expanded to hold large accessories and integrating spheres for spectral and diffuse reflectance. The LockDown mechanism makes it possible to quickly change and position accessories for reproducible results.
Features - Measure beyond 8.0 absorbance units with reference beam attenuation
- Measure 175 to 3300 nm using a PbSmart NIR detector for extended photometric range
- WinUV software—modular software with power analysis and enhanced transfer and report export capabilities
- Versatile set of accessories for materials research including spectral and diffuse reflectance
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with LockDown mechanism for quick changes and positioning for reproducible results
- Maximum light throughput using Schwarzchild coupling optics for higher accuracy at low transmission levels
- Minimal noise and stray light using a floating aluminum casting and double Littrow monochromator
- Extended dynamic range by attenuating the reference beam to balance double beam signal levels.
- Nitrogen purging—separate purging in the sample and monochromator compartments to reduce oxygen and water vapor in the ultra-violet.
... Read More | The Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, The Cary 6000i UV-Vis-NIR spectrophotometer has been tailored for unmatched photometric performance in the short-wave infrared (SWIR) 800–1800 nm range as well as the UV-visible 175 to 800 nm. Using narrow-band InGaAs detection and a 600 lines-per-mm diffaction grating for improved SWIR sensivity, the Cary 6000i is the ultimate tool for materials characterization spectroscopy.
Using Cary WinUV software with the Cary 6000i makes it easy to perform powerful analysis and control optional accessories. The large sample compartment can be expanded to hold large accessories and integrating spheres for spectral and diffuse reflectance. The LockDown mechanism makes it possible to quickly change and position accessories for reproducible results.
Features
- Measure beyond 8.0 absorbance units with reference beam attenuation
- Measure 175 to 1800 nm using a narrow-band InGaAs detector and 600 l/mm NIR diffraction gratings for unmatched sensivity in the short-wave infrared
- WinUV software—modular software with power analysis and enhanced transfer and report export capabilities
- Versatile set of accessories for materials research including spectral and diffuse reflectance
- Variable slit widths (down to 0.01 nm) for optimum control over data resolution
- Large sample compartment with LockDown mechanism for quick changes and positioning for reproducible results
- Maximum light throughput using Schwarzchild coupling optics for higher accuracy at low transmission levels
- Minimal noise and stray light using a floating aluminum casting and double out-of-plane Littrow monochromator
- Extended dynamic range by attenuating the reference beam to balance double beam signal levels.
- Nitrogen purging—separate purging in the sample and monochromator compartments to reduce oxygen and water vapor in the ultra-violet.
... Read More |
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