In Raman microscopy, also called confocal Raman microscopy, an optical microscope and a Raman spectrometer are combined into one instrument in order to provide high resolution images of small samples. This high resolution imaging has applications that include materials science, earth science, cell biology, medicine, cosmetics, and art analysis.
A Raman microscope consists of a basic optical microscope, a Raman spectrometer, a laser, a light source, and a detector such as a CCD camera. The optical microscope uses light to magnify and identify samples while the Raman spectrometer scatters light and measures the excitation vibration. When selecting a microscope, it is important to consider the qualities of each of the microscope's components and their optimal configuration for a particular application.
Find, compare, and request a quote for Raman and confocal Raman microscopes across leading suppliers at Labcompare; including access to product citations, related published figures, and first-hand reviews from our scientific community.
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| Company | WITec GmbH | WITec GmbH | WITec GmbH | WITec GmbH | WITec GmbH |
| Item | alpha300 R Confocal Raman Microscope | alpha300 Ri Inverted Confocal Raman Imaging Microscope | ParticleScout™ Automated Microparticle Analysis Tool | RISE™ – Raman Imaging and Scanning Electron Microscope | TrueSurface™ Microscopy Topographic Imaging System |
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| Catalog Number | alpha300 R | alpha300 Ri | ParticleScout™ | RISE™ Microscopy | TrueSurface™ |
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| Description | The confocal Raman microscope alpha300 R offers the unique ability to acquire chemical information non-destructively with a resolution down to the optical diffraction limit (~ 200 nm). This allows you to observe and analyze the distribution of different phases within a sample in ambient conditions The confocal Raman microscope alpha300 R offers the unique ability to acquire chemical information non-destructively with a resolution down to the optical diffraction limit (~ 200 nm). This allows you to observe and analyze the distribution of different phases within a sample in ambient conditions without specialized sample preparation. Because of the confocal setup, it is not only possible to collect information from the sample surface, but also to look deep inside transparent samples and even obtain 3D information.
A complete Raman spectrum is acquired at each image pixel, resulting in images consisting of tens of thousands of spectra. The acquisition time for one spectrum is only in the range of milliseconds, resulting in complete images being collected in a matter of minutes. When analyzing dedicated peak characteristics of the spectra, a variety of images can be generated using only a single set of data. This allows you not only to image the distribution of chemical compounds, but also to analyze, for example crystallinity or material stress properties. Further applications are typically found in polymer science, coating and thin film analysis, geoscience and in the pharmaceutical industry.... Read More | The WITec alpha300 Ri is an inverted confocal Raman imaging microscope that preserves all the functionality of WITec’s standard alpha300 series while introducing a new angle in access and handling. The microscope’s inverted beam geometry enables specimens in aqueous environments such as cell The WITec alpha300 Ri is an inverted confocal Raman imaging microscope that preserves all the functionality of WITec’s standard alpha300 series while introducing a new angle in access and handling. The microscope’s inverted beam geometry enables specimens in aqueous environments such as cell cultures to be examined more effectively. Standardized liquid sample holder formats can be quickly and easily mounted and measured, accelerating the experimental workflow and helping to ensure consistency. The alpha300 Ri is also compatible with other techniques including fluorescence, differential interference contrast (DIC) and phase-contrast microscopy.
Investigations in materials science will be aided by the very large working area that can accommodate bulky samples and the motorized sample stage also facilitates the mounting of environmental enclosures and other accessories. Research applications in life sciences, biomedicine and geosciences in particular will benefit from the consistency and flexibility provided by the geometry of the alpha300 Ri.... Read More | WITec’s ParticleScout is an advanced particle analysis tool for the alpha300 confocal Raman microscope series that finds, classifies and identifies microparticles over even large sample areas. Automated routines start by surveying samples with bright and dark field illumination to locate the WITec’s ParticleScout is an advanced particle analysis tool for the alpha300 confocal Raman microscope series that finds, classifies and identifies microparticles over even large sample areas. Automated routines start by surveying samples with bright and dark field illumination to locate the particles they contain. The particles of interest are sorted into a ranked list by user-defined physical attributes and their individual Raman spectra are acquired in rapid succession. This process automatically omits empty space and irrelevant particles, greatly reducing measurement time. The chemical identities of the particles can then be determined before a report is generated that provides a detailed quantitative overview of the sample. These reports make ParticleScout the perfect tool for finding correlations between the physical and chemical attributes of particles.
Investigations of particles in high-resolution is becoming increasingly important in fields such as environmental science, microplastics research, pharmaceutical research, medical device development, life sciences and many others. ParticleScout makes full use of Raman imaging’s advantages for fast, label-free and non-destructive chemical characterization, and its full integration with the TrueMatch Raman database management software offers a streamlined experimental environment to boost productivity. Any applications that require the quick and precise analysis of particulate samples will benefit from its analytical power and accelerated workflow.... Read More | RISE™ stands for Raman Imaging and Scanning Electron microscopy. Developed by WITec, it is the industry’s first production fully-integrated Raman-SEM system to use a common sample chamber for correlative measurements. It can automatically link ultra-structural surface properties to molecular RISE™ stands for Raman Imaging and Scanning Electron microscopy. Developed by WITec, it is the industry’s first production fully-integrated Raman-SEM system to use a common sample chamber for correlative measurements. It can automatically link ultra-structural surface properties to molecular compound information with software-controlled shuttling of the sample between two measuring positions. The RISE Microscope combines all features of a stand-alone SEM and the confocal Raman imaging microscope alpha300 within one instrument.... Read More | WITec´s True Surface Microscopy option (patent pending) allows confocal Raman imaging guided by surface topography. Confocal microscopy is often desirable due to its suppression of out-of-focus light but can be challenging when analyzing large or rough surfaces. In these cases, only those WITec´s True Surface Microscopy option (patent pending) allows confocal Raman imaging guided by surface topography. Confocal microscopy is often desirable due to its suppression of out-of-focus light but can be challenging when analyzing large or rough surfaces. In these cases, only those point s that are in focus contribute to the image. True Surface Microscopy follows the surface topography with high precision, so that even rough or inclined samples always stay in focus. To achieve this unique capability, the WITec alpha500 series can be equipped with a highly precise sensor for optical profilometry. The topographic coordinates from the profilometer measurement are used to perfectly follow the sample surface in confocal Raman imaging mode. The result is an image revealing chemical properties at the surface of the sample, even if this surface is rough or inclined.
Features - Extension for the WITec alpha500 series that combines Large Area Surface Topography profiling with Confocal Raman Imaging and/or AFM
- Unique combination (patent pending) delivers innovative application possibilities for new research techniques
- Ease-of-use through full integration with the alphaControl hardware and WITec Project Software environment
- Scan Speed up to 2000 pixels/s for rapid data acquisition
- Spatial Resolution of 10 - 25 µm laterally and < 100nm vertically to reveal an expanse of miniscule surface structures
- Light source: High brightness LED for highest throughput and accuracy
- Measuring distance: 10 mm – 16 mm providing wide-ranging sample size flexibility
- Multi-sensors easily configurable to meet virtually any application
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| Measurement Mode(s) | Confocal Raman Imaging Microscopy | Confocal Raman Imaging Microscopy | Confocal Raman Microscopy | Correlative Raman-SEM Imaging | Confocal Raman Imaging |
| Focal Length | Inquire | Inquire | Inquire | Inquire | less than 1 µm |
| Detector(s) | CCD/EMCCD | CCD/EMCCD | CCD/EMCCD | CCD/EMCCD | Optical Profilometer |
| Laser Sources | Multiple Laser Sources | Multiple Laser Sources | Multiple Laser Sources | Multiple Laser Sources | Multiple Laser Sources |
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