Spectrofluorometers are designed to detect and quantify fluorophores. These are fluorescent molecules that, when exposed to light, absorb photons at a characteristic wavelength. Subsequently, they then emit photons at a different and slightly longer characteristic wavelength. Spectrofluorometers contain excitation and emission filters for using different fluorophores. Monitoring the activities of molecules labeled with fluorophores is crucial to many kinds of experiments in cell biology, molecular biology and genetics, pharmaceuticals, and forensics, among many others. Emitted light may be detected by photomultiplier tubes or photodiodes within the spectrofluorometer, depending on the model. In addition, users of spectrofluorometers now have a wide range of high-tech fluorophores from which to choose to complement the features of a Spectrofluorometer, also referred to as fluorimeter. Modern fluorometers and fluorimeters are capable of detection as low as 1 part per trillion.
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| Company | Edinburgh Instruments Ltd | Edinburgh Instruments Ltd |
| Item | LP980 Spectrometer | LifeSpec-II Dedicated Lifetime Spectrometer |
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| Catalog Number | LP980 Spectrometer | LifeSpec-II |
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| Light Source | Flashlamp pumped Q-switched Nd:Yag laser operating at 1064 nm, 532 nm, 355 nm or 266 nm. | EPLs (Picosecond Pulsed Diode Lasers), EPLEDs (Picosecond Pulsed LEDs) |
| Wavelength Range | 200 nm – 870 nm | 200 to 1700 nm |
| Wavelength Accuracy | Inquire | Inquire |
| Scanning Speed | Inquire | Inquire |
| Description | At the heart of the LP980 is a fully integrated and sophisticated transient absorption spectrometer. It uses the pump-probe technique for measuring transient kinetics and sepctra. In kinetic mode (K) a single point detector is used to measure the transient kinetics at a single wavelength (spectra At the heart of the LP980 is a fully integrated and sophisticated transient absorption spectrometer. It uses the pump-probe technique for measuring transient kinetics and sepctra. In kinetic mode (K) a single point detector is used to measure the transient kinetics at a single wavelength (spectra can be built up by automated scanning over a wavelength range and slicing the data). In spectral mode (S) a gated intensified CCD is used to capture the whole spectrum in the presence and absence of the pump laser pulse, meaning the whole spectrum can be generated after one laser pulse. The LP980 spectrometer has a dual sample chamber that enables users to reliably and accurately measure transient chemical and biological species up to 2.55 µm using the pump-probe technique AND laser-induced fluorescence lifetimes down to nanosecond time ranges.
Transverse sample excitation geometry comes as standard. Thin-film, diffuse reflection, fluorescence and phosphorescence lifetime measurement accessories are available as options.... Read More | The LifeSpec-II is a compact, high performance computer controlled instrument for the acquisition and analysis of time resolved fluorescence, based on the technique of Time Correlated Single Photon Counting (TCSPC).
The instrument has been designed for operation with modern picosecond and The LifeSpec-II is a compact, high performance computer controlled instrument for the acquisition and analysis of time resolved fluorescence, based on the technique of Time Correlated Single Photon Counting (TCSPC).
The instrument has been designed for operation with modern picosecond and femtosecond lasers, such our EPLs (Picosecond Pulsed Diode Lasers), EPLEDs (Picosecond Pulsed LEDs), Titanium Sapphire lasers and supercontinuum sources. These sources enable measurements of fluorescence lifetimes down to tens of picoseconds across the UV-Visibile-NIR spectral regions up to 1700nm.
The LifeSpec-II has the unique feature of having an integrated emission monochromator with zero temporal dispersion. As such, very short fluorescence lifetimes can be recovered without an error introduced by the propagation of light on wavelength dispersive elements, such as the monochromator gratings.
The integrated system features a large sample compartment, with room for many sample holder options, including multi-position sample holders, cryostats, X-Y position sample holders, etc.
The instrument further comprises of computer controlled attenuation of incident light from the laser, optional polarisers, two computer selectable laser input ports and two computer controlled detector exit ports.
A detector shutter is both interlocked to the sample chamber for protection against excessive ambient light, and is also linked to the detector current to protect against overexposure by the laser.
The LifeSpec-II comes in two versions. The first version contains one set of gratings for the operation in the spectral range 200nm to 1000nm. The second version contains two sets of gratings for the operation in the spectral range 200nm to 1700nm.... Read More |
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