Spectrofluorometer

Spectrofluorometers are designed to detect and quantify fluorophores. These are fluorescent molecules that, when exposed to light, absorb photons at a characteristic wavelength. Subsequently, they then emit photons at a different and slightly longer characteristic wavelength. Spectrofluorometers contain excitation and emission filters for using different fluorophores. Monitoring the activities of molecules labeled with fluorophores is crucial to many kinds of experiments in cell biology, molecular biology and genetics, pharmaceuticals, and forensics, among many others. Emitted light may be detected by photomultiplier tubes or photodiodes within the spectrofluorometer, depending on the model. In addition, users of spectrofluorometers now have a wide range of high-tech fluorophores from which to choose to complement the features of a Spectrofluorometer, also referred to as fluorimeter. Modern fluorometers and fluorimeters are capable of detection as low as 1 part per trillion.

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CompanyEdinburgh Instruments LtdEdinburgh Instruments Ltd
ItemFS5 v2 SpectrofluorometerLP980 Spectrometer
Citations
Catalog NumberFS5 v2LP980 Spectrometer
Price
Light Source150 W CW Ozone-free xenon arc lamp (UV-enhanced ozone generating option available)Flashlamp pumped Q-switched Nd:Yag laser operating at 1064 nm, 532 nm, 355 nm or 266 nm.
Wavelength Range<230 nm to 1000 nm (Excitation), 200 nm to >870 nm (Emission)200 nm – 870 nm
Wavelength Accuracy± 0.5 nmInquire
Scanning Speed100 nm/sInquire
DescriptionCompact high-sensitivity spectrofluorometer; FS5 v2 is adaptable to a wide range of research and analytical applications.

Features:
  • High Sensitivity: Photon counting detection, with a signal-to-noise ratio of 12,000:1, provides exceptional sensitivity for the detection of weak fluorescence signals
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At the heart of the LP980 is a fully integrated and sophisticated transient absorption spectrometer. It uses the pump-probe technique for measuring transient kinetics and sepctra. In kinetic mode (K) a single point detector is used to measure the transient kinetics at a single wavelength (spectra ... Read More
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