Spectrofluorometers are designed to detect and quantify fluorophores. These are fluorescent molecules that, when exposed to light, absorb photons at a characteristic wavelength. Subsequently, they then emit photons at a different and slightly longer characteristic wavelength. Spectrofluorometers contain excitation and emission filters for using different fluorophores. Monitoring the activities of molecules labeled with fluorophores is crucial to many kinds of experiments in cell biology, molecular biology and genetics, pharmaceuticals, and forensics, among many others. Emitted light may be detected by photomultiplier tubes or photodiodes within the spectrofluorometer, depending on the model. In addition, users of spectrofluorometers now have a wide range of high-tech fluorophores from which to choose to complement the features of a Spectrofluorometer, also referred to as fluorimeter. Modern fluorometers and fluorimeters are capable of detection as low as 1 part per trillion.
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| Company | PicoQuant GmbH | PicoQuant GmbH |
| Item | FluoTime 300 | FluoTime 250 |
| Price | | |
| Light Source | Pulsed laser diode heads (LDH series, 375–1990 nm), pulsed LEDs (PLS series, 255–600 nm), ps laser modules (Prima, 375–640 nm), ps fiber-amplified laser systems (266–590 nm), 10W pulsed xenon lamp (200–1100 nm), 300W CW xenon lamp (200–1100 nm) | Laser diode heads (LDH series, 375–1990 nm), pulsed LEDs (PLS series, 255–600 nm, 400–1200 ps pulse width), ps laser modules (Prima, 375–640 nm) |
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