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  • Chambered Coverglass System

    Laetitia CLOTAIRE
    Tuesday, February 13, 2024
    For my immunofluorescence experiments, I used these coverglass chambers. It's a good system to do at the same time under different conditions. You can put your cells directly ... read more
  • Good Method to Study Mitochondrial Respiration

    Lisha Joshi
    Monday, February 12, 2024
    The energy metabolism of microglia activated with lipid biomolecule was measured using Agilent Seahorse XF analyzer. Serial addition of inhibitors of mitochondrial electron ... read more
  • Easy Glycolysis Assay Kit

    Lisha Joshi
    Monday, February 12, 2024
    Energy metabolism of microglia were studied with glycolysis assay kit. This kit measures the lactate secreted in the media by the cells treated with various substances. The ... read more
  • Assessing sGAG Using the Blyscan™ - sulfated Glycosaminoglycan (sGAG) Assay Kit

    Rachel Crossland
    Monday, February 12, 2024
    I have used the Blyscan™ - sulfated Glycosaminoglycan (sGAG) assay kit to assess GAG in chondrocytes isolated from osteoarthritis patients. The sGAG levels in the samples were... read more
  • Visualization of Nuclei Using DAPI

    Rachel Crossland
    Monday, February 12, 2024
    I have used the Fluoromount-G™ Mounting Medium, with DAPI to visualize nuclei. This was for human chondrocytes. I followed the manufacturer's instructions and visualized the ... read more
  • PKH26 Red Fluorescent Cell Linker Kit for Cell Membrane Staining

    Rachel Crossland
    Monday, February 12, 2024
    I have used the PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling for staining the membrane of extracellular vesicles derived from mesenchymal stem ... read more
  • Best Quality DPBS for Analytical Work

    SANDIP MHATARDEV GITE
    Monday, February 12, 2024
    I used this Phosphate-buffered saline (DPBS, 1X), Dulbecco's formula, without calcium, without magnesium as a mobile phase for my HPLC method development, and it was found to ... read more
  • X-tremeGENE 9 for Mammalian Cell Transfection

    Ganesh Pusapati
    Tuesday, February 06, 2024
    X-tremeGENE 9 is a well established transfection reagent for mammalian cell lines. I use this reagent at 1:3 (DNA:transfection reagent) for most of my experiments. The ... read more
  • X-tremeGENE HP Transfection Reagent

    Ganesh Pusapati
    Tuesday, February 06, 2024
    I have been using this product for over a decade for hard-to-transfect cell lines. I typically use this transfection reagent at 1:3 (DNA:reagent). I have noticed that this ... read more
  • siRNA Transfection Reagent

    Ganesh Pusapati
    Tuesday, February 06, 2024
    I use this product for transfecting siRNA in mammalian cell lines. I typically use it at 1:3 (siRNA:transfection reagent). I have tested it in HeLa and 3T3 cell lines and did ... read more
  • Genomic DNA Extraction Reagent

    Ganesh Pusapati
    Tuesday, February 06, 2024
    I use this reagent for extracting genomic DNA from cell lines. I typically use this reagent at 3-5x volume of cell pellet size. I strictly follow the manufacturer's ... read more
  • Transfection Reagent for 293-F Cell Line

    Ganesh Pusapati
    Tuesday, February 06, 2024
    I use this reagent for transfecting 293-F (suspension format) cell lines. I use the reagent at 3-4x of DNA amount (µL reagent: µg DNA). This is a great reagent for protein ... read more
  • Transfection Reagent for Insect Cell Expression

    Ganesh Pusapati
    Tuesday, February 06, 2024
    I use this reagent to transfect recombinant bacmid into Sf9 (insect) cells to generate P0 baculovirus. I typically seed 1 million cells per well in a 6-well plate and ... read more
  • Good Quality PCR Plates

    Neha Mittal
    Tuesday, January 30, 2024
    The skirted PCR plates are good to run samples at even high temperatures. It didn't spill the samples out or mix into other wells while handling the plates due to its firmness... read more
  • Cell Line Freezing Container

    Ganesh Pusapati
    Tuesday, January 30, 2024
    This is an 18-slot cryovial freezing container. I typically use 2-ml cryovials for freezing my cell lines. I place cryovials in this container (filled with fresh isopropanol) ... read more