Chromatography - A Separation Science
Chromatography is an analytical technique that allows the separation of analyte through its characteristics, such as - but not limited to - molecular charge, molecular size, or molecular affinity, to remain within the mobile phase, also known as the stationary phase. Once separated, the analyte can be isolated, purified, and quantified.
What are different types of chromatography?
There are several different setups to run a chromatographic separation. Based on the interaction of the analyte there is adsorption for polar non-ionic compounds, ion exchange for ionic compounds (anions and cations), partition based on solubility, size exclusion based on molecular size, and affinity with specific interactions (e.g. antibody interaction or protein interaction).
- Gas Chromatography (GC)
- Gas Adsorption Chromatography
- Gas-Liquid Partition Chromatography (GLPC)
- Capillary Gas Chromatography
- Liquid Chromatography (LC)
- Ion Exchange Chromatography (IC)
- Gel Permeation Chromatography (GPC)
- Affinity Chromatography
Applications of Chromatography
Chromatographic separation can be employed in various analytical testing laboratories, including:
- Environmental Testing
- Food and Beverage Analysis
- Forensic Tests
- Life Sciences
- And more
Range of Chromatography Instrumentation and Supplies
There are several general components to using chromatography in your laboratory. Based on the type of chromatography you'll be running in the lab will determine the type of chromatograph you will employ. The chromatograph is the instrument itself carrying out the operation. The chromatograph is used in conjunction with chromatography columns
. The stationary phase and mobile phase (e.g. Liquid or Gas) move through the columns. There are a variety of other accessories, including fittings, valves, fraction collectors
, and autosamplers available as well for increased throughput.
What to consider when choosing chromatography equipment and supplies?
Aside from determining the right type of chromatography for your separation needs, you'll want to consider chromatography columns that will be used in conjunction with your chromatograph. Additionally, a chromatography detector
is vital component in order to identify the molecules/sample that was chromatographically separated. General specifications to compare (varies between each type of chromatograph) include flow rate, maximum pressure, temperature range, # of injection ports, dimensions, and whether the system is capable of spectroscopic analysis (e.g. GC-MS, LC-MS, etc.).
Search and Compare Chromatography Equipment
The 2015 International Chemical Congress of Pacific Basin ... read more
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The 2015 International Chemical Congress of Pacific Basin Societies (Pacifichem), will take place in Honolulu, Hawaii, USA, December 15-20, 2015. The conference is sponsored jointly by the American Chemical Society (ACS), the Canadian Society for ...
As the premier international meeting in the field of cell biology, the ASCB Annual Meeting is intended for scientists and students in academia, industry, government, and higher education. Over 100 scientific sessions and 2,500 poster presentations ...
Practichem has launched the Arista Slice, which shrinks biochromatography control workstations and allows for the use of compact slices (22 cm wide). Each Arista Slice integrates gradient buffer pumps with buffer valves, a column selector, an injection valve, LED-based UV and conductivity monitoring... read more
Wednesday, December 17, 2014
VICI’s Pulsed Discharge helium ionization Detector (PDD) extends the detection capabilities of the popular Agilent 6890 and new Agilent 7890 GCs. The basic operating mode of the PDD can replace many of the detectors currently used by GC manufacturers. read more
Scientists and students in the Research Triangle area of North Carolina will have new opportunities to attend presentations by leading analytical chemistry authorities, thanks to an endowment established by Thermo Fisher Scientific in the College of Sciences at North Carolina State University. The ... read more
Shimadzu Scientific Instruments has launched the GCMS-TQ8040 with Smart MRM. Featuring Smart Productivity for high-efficiency sample throughput, Smart Operation for quick and easy method development, and Smart Performance for low detection limits and Scan/MRM, the GCMS-TQ8040 provides the most ... read more
Supercritical Fluid Technologies, Inc. introduces a new generation of the SFT-150 Supercritical Fluid Extraction (SFE) System. Developed to investigate the application of supercritical fluid techniques to various analyses and processing situations, the SFT-150 SFE is a full capability, research ... read more