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Comprising two components, a liquid-phase separation system and a mass spectrometer, liquid chromatograph mass spectrometers (LC MS instruments) couple sample fractionation and analysis. The separation can be accomplished via standard liquid chromatography, HPLC, UPLC, or even nano-LC. Any liquid-phase separation will do, so long as its output can be directed into the mass analyzer (for instance, via an electrospray ionization source). The mass spectrometer is similarly flexible, ranging from the simplest single quadrupole to a Fourier transform ion cyclotron resonance instrument. Just remember, in a liquid chromatograph mass spectrometer the column flow is continuous, meaning the mass analyzer must be fast enough to thoroughly analyze one peak before the next one comes off the column. And, unlike with MALDI MS, once a peak is analyzed, it’s gone.
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- Ionization Method(s) : ESI, APCI
- Mass Analyzer : Quadrupole, TOF, Ion Trap, QQQ or Q-TOF
- Scanning Speed : up to 5250 u/sec
- Resolution : Inquire
- Ionization Method(s) : ESI, APCI, APPI
- Mass Analyzer : Single Quadrupole
- Scanning Speed : 10,000 amu/sec (Model 6140)
- Resolution : Inquire
- Ionization Method(s) : Electrospray (ESI)
- Mass Analyzer : Time of Flight
- Scanning Speed : up to 40 spectra per sec.
- Resolution : > 20,000 Resolving Power
- Ionization Method(s) : ESI, APCI
- Mass Analyzer : Ion Trap
- Scanning Speed : 800 to 27,000 u/s (Depending on model)
- Resolution : < 0.35 to 4 FWHM (u) (depending on model, scan speed and m/z)
- Ionization Method(s) : ESI, APCI, APPI
- Mass Analyzer : Triple Quadrupole (QqQ)
- Scanning Speed : Inquire
- Resolution : Inquire
- Ionization Method(s) : ESI, APCI, APPI and MALDI
- Mass Analyzer : Quadrupole Time of Flight (QTOF)
- Scanning Speed : up to 20 Spectra per sec.
- Resolution : up to 40,000 Mass Resolution (depending on model)
- Ionization Method(s) : Electrospray (ESI), Atmospheric Pressure Chemical Ionization (APCI)
- Mass Analyzer : Quadrupole
- Scanning Speed : Inquire
- Resolution : Inquire
- Ionization Method(s) : ESI and/or APCI
- Mass Analyzer : Single Quadrupole
- Scanning Speed : 15,000 u/sec
- Resolution : Inquire
- Ionization Method(s) : APCI or ESI
- Mass Analyzer : MS/MS: Ion Trap/TOF
- Scanning Speed : Inquire
- Resolution : > 1,000 at 1,000 m/z
- Ionization Method(s) : ESI or APCI
- Mass Analyzer : Triple Quadrupole
- Scanning Speed : Inquire
- Resolution : Inquire
- Ionization Method(s) : ESI or APCI
- Mass Analyzer : Ion Trap
- Scanning Speed : 15,000 Da per sec.
- Resolution : Unit Mass Resolution
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