The Agilent 8890 GC system and Agilent 5977 GC/MSD system were used for the screening of pesticides in strawberries. With proper selection of instrument configuration, operating conditions, sample preparation, and software workflow, the system provides a robust way to identify pesticides in complex matrices. The hardware was optimized for pesticide detection in foods by incorporating pulsed splitless injection, midcolumn backflush, the Inert Extractor EI source, and retention time locking to a database of pesticides and environmental pollutants. The complete analysis was done in two steps. This application note describes the first step in this workflow whereby samples are screened using Agilent MassHunter Unknowns Analysis software, which provides automated deconvolution and library searching to identify any pesticides or other chemicals of concern.
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