| Detector(s) | Differential Refractive Index (dRI) | High-gain, high-dynamic-range photodiodes at 18 angles for MALS; optional avalanche photodiode that may be placed at any of four angles for DLS.
Fluorescence-blocking filters available for use with analytes that fluoresce when illuminated at the MALS wavelength. | High-gain, high dynamic range, static light scattering detectors at 3 angles | Differential Refractive Index (dRI) | Avalanche photodiode coupled to a multimode optical fiber |
| Applications | Measure concentrations of all types of samples in almost all solvents | With standard SEC/GPC or EclipseTM (FFF system): determination of distributions of molar mass, size, conformation, conjugation ratio and particle concentration.
With Calypso composition-gradient system: label-free characterization of biomolecular interactions including self- and hetero-association as well as non-specific interactions. | With SEC/GPC: determination of distributions of absolute molar mass, size, conformation, conjugate ratio. | GPC/SEC analysis of macromolecules by SEC-MALS | High-resolution size distributions with fractionation by SEC or FFF; conformation when combined with MALS molar mass and/or size data |
| Description | The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4The microOptilab™ brings universal detection capabilities, unsurpassed concentration range, and superb sensitivity and linearity to the domain of UHPLC. Key benefits include low band broadening, < 4 µL, to maintain the resolution of very narrow UHPLC peaks. Its dRI measurement range is, - 4.7 x 10-3 to + 4.7 x 10-3 RIU (comparable to 25 mg/mL protein in PBS). The detector is highly sensitive, ± 1.5 x 10-9 RIU, across the entire measurement range. This detector measures solvent refractive index, required for MALS calculations at the same wavelength as the MALS detector, for maximum accuracy. The temperature range of 4°C - 65°C is appropriate for both proteins and polymers that require a range of temperatures. It also features a high-resolution, touch-screen front panel displays data graphs, system health indicators and instrument diagnostics.... Read More | The DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar massThe DAWN™ instrument is an advanced detector for precise characterization of proteins, polymers, and nanoparticles including viral vectors, lipid nanoparticles and vesicles. Using multi-angle static light scattering (MALS) and optional dynamic light scattering (DLS), DAWN analyzes molar mass, size, conformation, particle concentration, conjugation (e.g. glycoproteins) or encapsulation (e.g. AAV capsid content). It stands out with exceptional sensitivity and the widest range of molecular weight, size, and concentration of any MALS instrument, paired with a broad selection of configurations and optional modules for enhanced capabilities.
The DAWN is most often used in conjunction with gel permeation chromatography (GPC-MALS), size exclusion chromatography (SEC-MALS) or an EclipseTM, field-flow fractionation instrument (FFF-MALS) and may also be used with ion-exchange chromatography or reverse-phase chromatography. The instrument includes a high-resolution, touch-screen front panel to display data graphs, system health and instrument diagnostics and is accompanied by ASTRA™, the most versatile software package available for analysis of online multi-angle and dynamic light scattering.... Read More | The microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size andThe microDAWN™ MALS detector performs absolute characterization of macromolecules eluting from UHPLC (or UPLC), determining molar mass and size independently of retention time and molecular reference standards. This instrument offers superb sensitivity over a wide range of molar mass, size and concentrations. In most respects, the microDAWN is similar to the well-established miniDAWN, a workhorse MALS instrument used in hundreds of labs around the world for characterizing proteins and other molecules smaller than 50 nm in radius. The microDAWN is uniquely suitable for use in conjunction with UHPLC Size Exclusion Chromatography (µSEC-MALS) thanks to its small interdetector dispersion and produces minimal band broadening in order to maintain the narrow peaks typical of UHPLC. In some cases it may be used with ion-exchange or other types of chromatography.... Read More | The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same The Optilab™ is a unique dRI detector for standard HPLC/GPC with 256 times the detection power and 50 times the dynamic range over alternative RI detectors for chromatography. This detector measures the solvent's absolute refractive index, a requirement for MALS calculations, at the same wavelength as the MALS detector, for maximum accuracy. With an operating window from 4°C - 65°C it is appropriate for both protein and polymer samples. Key benefits include its extraordinary range: -0.0047 to +0.0047 RIU (comparable to 25 mg/mL protein in PBS); the high-concentration version extends the range by 7x. Its sensitivity is ± 7.5 x 10-10 RIU across the entire measurement range. It also includes a high-resolution, touch-screen front panel that displays data graphs and diagnostics.... Read More | The WyattQELS™ dynamic light scattering (DLS) module integrates into a DAWN™, miniDAWN™ or microDAWN™ online multi-angle light scattering (MALS) instrument in order to determine high-resolution particle size distributions, when used in conjunction with a separation technique The WyattQELS™ dynamic light scattering (DLS) module integrates into a DAWN™, miniDAWN™ or microDAWN™ online multi-angle light scattering (MALS) instrument in order to determine high-resolution particle size distributions, when used in conjunction with a separation technique such as size exclusion chromatography (SEC) or Eclipse™, field-flow fractionation (FFF). The measurement is performed at exactly the same time and in the same sample volume as MALS so Rh and Rg can be compared for particle conformation insights.
Applications include the characterization of the conformation of polymers and biomolecules, detection of protein unfolding, and identification of nanoparticles and vesicles based on shape and structure. One can also use QELS insights to determine optimal chromatography conditions based on molecular conformation.... Read More |