Gel permeation chromatography (GPC), a subset of size-exclusion chromatography (SEC), is used to separate samples by size. This is most often used for analytes consisting of polymers. A laser light scattering detector can be used with GPC to measure molecular weight of the sample directly. This is done by passing a laser light through the separated sample in the column and measuring the amount of light scattered which is directly proportional to the molecular weight. GPC laser light scattering detectors can be purchased with a single detector system or with multiple detectors, such as with viscometry or concentration detectors, for readouts with additional details.
|  |  |
| Company | Malvern Panalytical | Malvern Panalytical |
| Item | OMNISEC GPC/SEC UHPLC/UPLC Multi-Detector System | OMNISEC REVEAL GPC/SEC System |
| Citations | | |
| Reviews | | |
| Catalog Number | OMNISEC | OMNISEC REVEAL |
| Price | | |
| Description | Meet the world’s most advanced multi-detector GPC/SEC system, OMNISEC. In OMNISEC, Malvern's expertise has enabled the development of the most sensitive and accurate UHPLC/UPLC multi-detector GPC/SEC system available for protein characterization and polymer characterisation, both synthetic and Meet the world’s most advanced multi-detector GPC/SEC system, OMNISEC. In OMNISEC, Malvern's expertise has enabled the development of the most sensitive and accurate UHPLC/UPLC multi-detector GPC/SEC system available for protein characterization and polymer characterisation, both synthetic and natural.
OMNISEC includes OMNISEC RESOLVE, the integrated GPC/SEC module, OMNISEC REVEAL, the integrated multi-detector module and OMNISEC software for the characterization of synthetic and natural polymers, and proteins.
OMNISEC RESOLVE combines an integrated pump, degasser, autosampler and column oven in a single advanced separations unit.
OMNISEC REVEAL is the most advanced integrated GPC/SEC multi-detector available, incorporating refractive index, UV/Vis absorbance, light scattering and intrinsic viscosity detectors.
Features:
- Optimized isocratic pump and improved degassing efficiency in OMNISEC RESOLVE offers unrivalled flow rate stability to reduce baseline noise in all detectors
- Integrated column oven in OMNISEC RESOLVE maintains a stable separation temperature, all the way up to 65°C
- Autosampler with zero injection volume overhead mode prevents wastage of your most precious samples, and can cool fragile samples to 4°C to protect them from aggregation and degradation
- OMNISEC REVEAL's light scattering detector is the most sensitive available enabling measurements of molecular weights as low as 200 Da and injection masses as low as 100 ng
- The self-balancing differential viscometer in OMNISEC REVEAL has user exchangeable capillaries to make it more robust and usable than any other available
- Combine data from multiple detectors to calculate hydrodynamic radius (Rh), radius of gyration (Rg), and Mark-Houwink parameters
- SEC-MALs detector that can be used to measure the absolute molecular weight of proteins, synthetic and natural polymers, as well as molecular size expressed as the radius of gyration, Rg.
*UHPLC - ultrahigh performance liquid chromatography
*UPLC - ultra performance liquid chromatography... Read More | Incorporating refractive index, UV/Vis absorbance, light scattering and intrinsic viscosity detectors, OMNISEC REVEAL is the most advanced integrated GPC/SEC multi-detector available. It offers comprehensive molecular characterization of synthetic and natural polymers as well as proteins.
Sold as Incorporating refractive index, UV/Vis absorbance, light scattering and intrinsic viscosity detectors, OMNISEC REVEAL is the most advanced integrated GPC/SEC multi-detector available. It offers comprehensive molecular characterization of synthetic and natural polymers as well as proteins.
Sold as part of a complete OMNISEC system, or as a stand-alone module for connection to any existing GPC/SEC system, OMNISEC REVEAL is a complete integrated multi-detector module for advanced GPC/SEC analysis. It includes refractive index (RI), UV/Vis absorbance, light scattering and intrinsic viscosity detectors for the characterization of synthetic polymers, natural polymers and polysaccharides, and proteins.
In combination, these detectors can measure multiple molecular properties including concentration, dn/dc, absolute molecular weight, intrinsic viscosity, molecular size (Rh and Rg), and Mark-Houwink parameters. A temperature controlled detector compartment (20 to 65°C) improves baseline stability and minimizes the amount of inter-detector tubing and thus band-broadening that occurs between the detectors.
OMNISEC REVEAL can be used to relate a polymer’s molecular properties to its bulk characteristics in applications from packaging and energy to food and pharmaceuticals. Its protein characterization capabilities can be applied to academic research and biopharmaceutical development.
Features:- OMNISEC REVEAL's light scattering detector is the most sensitive available enabling measurements of molecular weights as low as 200 Da and injection masses as low as 100 ng. It combines the sensitivity of 90° Right Angle Light Scattering (RALS) with the accuracy of 7° Low-Angle Light Scattering (LALS) to achieve the ultimate sensitivity and accuracy. Its flow cell is just 18 µL minimizing band broadening
- The self-balancing differential viscometer in OMNISEC REVEAL has user exchangeable capillaries to make it more robust and usable than any other available. Its 316 stainless steel constructions means few limitations with salts of pH and it has an exchangeable bridge for fast user replacement and the ability to self-balance for simple, automatic setup
- The refractive index detector measures the concentration of almost any solute. Its robust design allows it to be the first detector in series, minimizing band broadening and maximizing measurement quality and sensitivity
- The UV/Vis photodiode array (PDA) covers the full UV and visible spectra (200-900 nm)
- Combine data from multiple detectors to calculate hydrodynamic radius (Rh), radius of gyration (Rg), and Mark-Houwink parameters
... Read More |
| Weight Range | Inquire | 40 kg |
| Temperature Range | 20°C to 65 °C | 20°C to 65 °C |
| Radius of Gyration Range | Inquire | Inquire |
| Get Quote | | |