| Description | Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the SpectraMax® MiniMaxTM imaging cytometer option adds first-of-its-kind cellular imaging capability to a microplate reader. The upgradable option makes cytometry more accessible to a wider audience, providing researchers Designed specifically for the SpectraMax® i3 Multi-Mode Detection Platform, the SpectraMax® MiniMaxTM imaging cytometer option adds first-of-its-kind cellular imaging capability to a microplate reader. The upgradable option makes cytometry more accessible to a wider audience, providing researchers with cellular analysis capability without the need for investment in complex imaging systems. With brightfield and fluorescent imaging modes, the imaging cytometry option enables quick inspection of cells and provides insight into phenotypic changes that are important for understanding cytotoxicity, cell proliferation, and protein expression.
Features - Simple workflow: Cell and whole-well assays in a small footprint
- More data: Multi-parametric cell-based assays with brightfield and fluorescent modes
- Familiar software: Set up, image and analyze cells in a snap using SoftMax® Pro Software
- Higher sensitivity: Use fewer and or less fluorescent cells compared to whole-well measurements
- Lower variance: Normalize readings with number of cells in a well
... Read More | Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Features :
- White light and fluorescent imaging -
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green). -
Data tools to accelerate research timelines -
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing. -
Quickly image a variety of plate formats and cell types -
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types. -
Intelligent analysis with easy-to-use software -
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training. -
High-resolution images -
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days. -
Custom automation options -
The Automation and Customization Team offers a variety of custom services from robotic plate loading to fully automated workstations with liquid handling and incubation. We have built out custom automated work cells with the CloneSelect Imager for iPSC workflows, full assay systems and drug toxicity and characterization research.
... Read More | The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader The SpectraMax® i3 Multi-Mode Detection Platform comes with standard spectral absorbance, fluorescence, and luminescence detection. Additionally, user-installable options allow the SpectraMax i3 System to grow with your changing application needs to fulfill and go beyond the standard reader applications, protecting your intial investment. As new assays are developed or your needs change, simply add a cartridge or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system.
Features - User-Upgradeability Expand the 3-mode base system with additional application cartridges for FP, HTRF®, AlphaScreen® assays, and cellular imaging
- SpectraMax® MiniMaxTM Imaging Cytometer simplifies complex imaging workflow by visualizing cell morphology changes on top of well-based intensity readings
- Spectral FusionTM Illumination delivers a high-powered, full spectrum light source for increased fluorescence performance across the entire excitation range
- Expanded Dynamic Range: sophisticated engineering delivers optimal sensitivity and maximizes signal range
- SoftMax® Pro Microplate Data Acquisition & Analysis Software: Industry-leading SoftMax Pro Software streamlines complex data analysis with over 140 built-in protocols, effectively eliminating the need for additional software. Additional tools for regulatory compliance are included in SoftMax Pro GxP Software
... Read More | Flash and glow luminescence detection in an upgradeable platform.
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold Flash and glow luminescence detection in an upgradeable platform.
The SpectraMax® L Microplate Reader measures flash and glow luminescence assays in both 96- and 384-well plates. It is upgradeable to include a barcode reader, programmable injectors, and dual-channel configurations for a 2-fold increase for medium throughput. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume injector configuration make this reader ideal for fast kinetic assays requiring continuous monitoring during injection and measurement.
Reduced noise -
Ultra-fast photon counting technology amplifies the PMT signal with a fast pulse amplifier. This reduced noise capability enables a higher signal-to-noise ratio and low crosstalk.
PMT calibration -
The automatic PMT calibration method is based on readings of 4 internal LEDs at 395, 470, 527, and 570 nm. The manual PMT calibration method is based on readings of 4 wells from the assay.
Other features and benefits include:
- Detect all samples
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Get 2-fold improvement in speed
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Automate injector workflows
... Read More | Dual monochromators increase performance and assay flexibility.
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm Dual monochromators increase performance and assay flexibility.
The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. These robust readers have been placed in labs from Antarctica to the International Space Station.
With optimized reagents, validation tools, and industry-leading SoftMax® Pro Software, they provide consistent performance.
Patented optimization -
The patented AutoPMT optimization of the SpectraMax M5 reader adjusts the fluorescence detector to each sample well's concentration and normalizes the raw data, extending the dynamic range of assays.
IQ/OQ/PM Services -
Qualify Molecular Devices microplate readers in GLP or GMP environments with our IQ/OQ/PM Services to preserve the documentation of services in a compliant format that can be accessed remotely.
Validation tools -
Extensive suite of validation tools reduces the cost and time of validation by 50% as compared to using multiple platforms to collect and analyze data.
Compliance software -
SoftMax Pro GxP Software extends data acquisition and analysis solution into regulated laboratories working under GMP, GLP, and 21 CFR Part 11 for secure electronic records.
Other features and benefits include: - - Assay flexibility
- Better absorbance accuracy
- Increased throughput
... Read More |